This research using the animal model with spina bifida, which is one of the congenital malformation of a partial spinal cord, was operated to investigate the pathophysiology of the leg dysfunction in this model, using clues to understanding the nature of the neuronal development in the normal and/or abnormal the spinal cord region. On the other hand, in comparing the pathological condition of the spina bifida model animal, which is the final objective of this study, with the pathological condition of human spina bifida, the model used from the beginning caused inconvenience. Therefore, we decided to improve the method of creating the spina bifida model in this study. As a result, it has become possible to create a model that exhibits symptoms that are more similar to those of human spina bifida. It was suggested that the addition of this model to the experimental subjects may lead to more socially meaningful results.