2007年8月
Identification of homozygous deletions of tumor suppressor gene FAT in oral cancer using CGH-array
ONCOGENE
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- 巻
- 26
- 号
- 36
- 開始ページ
- 5300
- 終了ページ
- 5308
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1038/sj.onc.1210330
- 出版者・発行元
- NATURE PUBLISHING GROUP
Homozygous deletions (HD) provide an important resource for identifying the location of candidate tumor suppressor genes. To identify the tumor suppressor gene in oral cancer, we employed high-resolution comparative genomic hybridization (CGH)-array analysis. We identified a homozygous loss of FAT ( 4q35), a new member of the human cadherin superfamily, from genome-wide screening of copy number alterations in one primary oral cancer. This result was evaluated by genomic polymerase chain reaction in 13 oral cancer cell lines and 20 primary oral cancers and Southern blot in the cell lines. We found frequent exonic HD of FAT in the cell lines (3/13, 23%) and in primary oral cancers (16/20, 80%). FAT expression was absent in these cell lines. Homozygous deletion hot spots were observed in exon 1 (9/20, 45%) and exon 4 (7/20, 35%). Moreover, loss of gene expression was identified in other types of squamous cell carcinoma. The methylation status of the FAT CpG island in squamous cell carcinomas correlated negatively with its expression. Our results identify mutations in FAT as an important factor in the development of oral cancer and indicate the importance of FATs function in some squamous cell carcinomas.
- リンク情報
- ID情報
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- DOI : 10.1038/sj.onc.1210330
- ISSN : 0950-9232
- Web of Science ID : WOS:000248674200013