2018年4月1日
Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis
Genes to Cells
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- 巻
- 23
- 号
- 4
- 開始ページ
- 318
- 終了ページ
- 325
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/gtc.12573
- 出版者・発行元
- Blackwell Publishing Ltd
We developed the engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) technology to isolate specific genomic regions while retaining their molecular interactions. In enChIP, the locus of interest is tagged with an engineered DNA-binding molecule, such as a modified form of the clustered regularly interspaced short palindromic repeats (CRISPR) system containing a guide RNA (gRNA) and a catalytically inactive form of Cas9 (dCas9). The locus is then affinity-purified to enable identification of associated molecules. In this study, we generated transgenic mice expressing 3xFLAG-tagged Streptococcus pyogenes dCas9 (3xFLAG-dCas9) and retrovirally transduced gRNA into primary CD4+ T cells from these mice for enChIP. Using this approach, we achieved high yields of enChIP at the targeted genomic region. Our novel transgenic mouse lines provide a valuable tool for enChIP analysis in primary mouse cells.
- リンク情報
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- DOI
- https://doi.org/10.1111/gtc.12573
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/29480524
- URL
- http://orcid.org/0000-0003-1296-4256
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042424120&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85042424120&origin=inward
- ID情報
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- DOI : 10.1111/gtc.12573
- ISSN : 1365-2443
- ISSN : 1356-9597
- eISSN : 1365-2443
- ORCIDのPut Code : 42059872
- PubMed ID : 29480524
- SCOPUS ID : 85042424120