2015年1月
A Critical Role of the Thy28-MYH9 Axis in B Cell-Specific Expression of the Pax5 Gene in Chicken B Cells
PLOS ONE
- ,
- ,
- 巻
- 10
- 号
- 1
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0116579
- 出版者・発行元
- PUBLIC LIBRARY SCIENCE
Accumulating evidence suggests that Pax5 plays essential roles in B cell lineage commitment. However, molecular mechanisms of B cell-specific expression of Pax5 are not fully understood. Here, we applied insertional chromatin immunoprecipitation iChIP) combined with stable isotope labeling using amino acids in cell culture (SILAC) (iChIP-SILAC) to direct identification of proteins interacting with the promoter region of the endogenous single-copy chicken Pax5 gene. By comparing B cells with macrophage-like cells trans-differentiated by ectopic expression of C/EBP beta, iChIP-SILAC detected B cell-specific interaction of a nuclear protein, Thy28/Thyn1, with the Pax5 1A promoter. Trans-differentiation of B cells into macrophage- like cells caused down-regulation of Thy28 expression. Loss-of-function of Thy28 induced decrease in Pax5 expression and recruitment of myosin-9 (MYH9), one of Thy28-interacting proteins, to the Pax5 1A promoter. Loss-of-function of MYH9 also induced decrease in Pax5 expression. Thus, our analysis revealed that Thy28 is functionally required for B cell-specific expression of Pax5 via recruitment of MYH9 to the Pax5 locus in chicken B cells.
- リンク情報
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- DOI
- https://doi.org/10.1371/journal.pone.0116579
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/25607658
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4301804
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000348205300029&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/25607658
- URL
- http://orcid.org/0000-0003-1296-4256
- ID情報
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- DOI : 10.1371/journal.pone.0116579
- ISSN : 1932-6203
- ORCIDのPut Code : 49884985
- PubMed ID : 25607658
- PubMed Central 記事ID : PMC4301804
- Web of Science ID : WOS:000348205300029