Jul, 2014
Identification of Proteins Associated with an IFN gamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR
PLOS ONE
- ,
- Volume
- 9
- Number
- 7
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1371/journal.pone.0103084
- Publisher
- PUBLIC LIBRARY SCIENCE
Isolation of specific genomic regions retaining molecular interactions is essential for comprehensive identification of molecules associated with the genomic regions. Recently, we developed the engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) technology for purification of specific genomic regions. Here, we developed a retroviral expression system for enChIP using CRISPR. We showed that the target genomic locus can be purified with high efficiency by using this system. We also showed that contamination of potential off-target sites is negligible by using this system if the guide RNA (gRNA) for the target site has a sufficiently long unique sequence in its seed sequence. enChIP combined with stable isotope labeling using amino acids in cell culture (SILAC) analysis identified proteins whose association with the interferon (IFN) regulatory factor-1 (IRF-1) promoter region increases in response to IFN gamma stimulation. The list of the associated proteins contained many novel proteins in the context of IFN gamma-induced gene expression as well as proteins related to histone deacetylase complexes whose involvement has been suggested in IFN gamma-mediated gene expression. Finally, we confirmed IFN gamma-induced increased association of the identified proteins with the IRF-1 promoter by ChIP. Thus, our results showed that the retroviral enChIP system using CRISPR would be useful for biochemical analysis of genome functions including transcription and epigenetic regulation.
- Link information
-
- DOI
- https://doi.org/10.1371/journal.pone.0103084
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/25051498
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106880
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000339551100086&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/25051498
- URL
- http://orcid.org/0000-0003-1296-4256
- ID information
-
- DOI : 10.1371/journal.pone.0103084
- ISSN : 1932-6203
- ORCID - Put Code : 49884989
- Pubmed ID : 25051498
- Pubmed Central ID : PMC4106880
- Web of Science ID : WOS:000339551100086