Shinya Fushinobu

Profile Information

Affiliation: Professor, Graduate School of Agricultural and Life Sciences, Biotechnology, The University of Tokyo

Degree: Doctor(Agriculture)(The University of Tokyo)

Researcher number: 00302589
ORCID iD: https://orcid.org/0000-0003-1346-6435
J-GLOBAL ID: 200901011725464371
researchmap Member ID: 1000255551

External link: http://enzyme13.bt.a.u-tokyo.ac.jp/fushi/index-e.html

Research Interests

Research Areas

Research History

Dec, 2012 - Present

Professor, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Apr, 2011 - Nov, 2012

Associate Professor, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Apr, 2007 - Apr, 2011

Assistant Professor, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Nov, 1997 - Mar, 2007

Assistant Professor, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Education

- 1996

The University of Tokyo
- 1994

The University of Tokyo

Committee Memberships

Dec, 2023 - Present

Editorial Board Member, Microbiome Research Reports
Sep, 2023 - Present

director, JSAG
May, 2023 - Present

Editorial Board Member of The Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology (ASBMB)
May, 2023 - Present

Kanto branch head, JSBBA
Feb, 2022 - Present

Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (NC-IUBMB), Enzyme subcommittee member
2016 - Present

Editorial Board Member, Applied Microbiology and Biotechnology
Jan, 2016 - Present

Editorial Board Member of Applied and Environmental Microbiology, American Society for Microbiology (ASM)
Jun, 2021 - Jun, 2024

officer, PSSJ

Awards

Papers

309

Identification and Structural Basis of Novel Enzymes that Degrade Cell Wall Glycans of Mycobacteria

Toma KASHIMA, Michiko SHIMOKAWA, Akihiro ISHIWATA, Kiyotaka FUJITA, Shinya FUSHINOBU

Nihon Kessho Gakkaishi, 66(3) 171-172, Aug 31, 2024
Genetic and functional diversity of β-N-acetylgalactosamine-targeting glycosidases expanded by deep-sea metagenome analysis

Tomomi Sumida, Satoshi Hiraoka, Keiko Usui, Akihiro Ishiwata, Toru Sengoku, Keith A. Stubbs, Katsunori Tanaka, Shigeru Deguchi, Shinya Fushinobu, Takuro Nunoura

Nature Communications, May 10, 2024
Cytosolic <scp>UDP‐L</scp>‐arabinose synthesis by bifunctional <scp>UDP</scp>‐glucose 4‐epimerases in Arabidopsis

Akira Umezawa, Mayuko Matsumoto, Hiroto Handa, Konatsu Nakazawa, Megumi Miyagawa, Georg J. Seifert, Daisuke Takahashi, Shinya Fushinobu, Toshihisa Kotake

The Plant Journal, Apr 28, 2024
Taking a walk to find new mucinases

Shinya Fushinobu

Nature Catalysis, Apr 26, 2024
Role of β-L-Arabinofuranosidases in Intestinal Bacteria

Fujita, K, Ishiwata, A, Fushinobu, S

Trends in Glycoscience and Glycotechnology, 36(210) E35-E38, Mar 25, 2024 Peer-reviewedInvited
N²‐Acetylornithine deacetylase functions as a <scp>Cys‐Gly</scp> dipeptidase in the cytosolic glutathione degradation pathway in Arabidopsis thaliana

Shunsuke Miyaji, Takehiro Ito, Taisuke Kitaiwa, Kosuke Nishizono, Shin‐Ichiro Agake, Hiroki Harata, Haruna Aoyama, Minori Umahashi, Muneo Sato, Jun Inaba …

The Plant Journal, Mar 5, 2024
Structural insights into thermophilic chaperonin complexes

Zengwei Liao, Chai C. Gopalasingam, Masafumi Kameya, Christoph Gerle, Hideki Shigematsu, Masaharu Ishii, Takatoshi Arakawa, Shinya Fushinobu

Structure, Mar, 2024
Charge neutralization and β-elimination cleavage mechanism of family 42 L-rhamnose-α-1,4-D-glucuronate lyase revealed using neutron crystallography

Naomine Yano, Tatsuya Kondo, Katsuhiro Kusaka, Takatoshi Arakawa, Tatsuji Sakamoto, Shinya Fushinobu

Journal of Biological Chemistry, Mar, 2024
Bifidobacterial GH146 β-l-arabinofuranosidase for the removal of β1,3-l-arabinofuranosides on plant glycans

Kiyotaka Fujita, Hanako Tsunomachi, Pan Lixia, Shun Maruyama, Masayuki Miyake, Aimi Dakeshita, Kanefumi Kitahara, Katsunori Tanaka, Yukishige Ito, Akihiro Ishiwata …

Applied Microbiology and Biotechnology, 108 199, Feb 7, 2024 Peer-reviewed
Molecular Basis of Absorption at 340 nm of 3-Ketoglucosides under Alkaline Conditions.

Motomitsu Kitaoka, Ayu Takano, Mei Takahashi, Yoshiki Yamakawa, Shinya Fushinobu, Nobuyuki Yoshida

Journal of applied glycoscience, 71(1) 9-13, 2024
Crystal Structure of <i>Bifidobacterium bifidum</i> Glycoside Hydrolase Family 110 α-Galactosidase Specific for Blood Group B Antigen

Toma Kashima, Megumi Akama, Takura Wakinaka, Takatoshi Arakawa, Hisashi Ashida, Shinya Fushinobu

Journal of Applied Glycoscience, 2024
Protocatechuate hydroxylase is a novel group A flavoprotein monooxygenase with a unique substrate recognition mechanism

Nozomi Katsuki, Riku Fukushima, Yuki Doi, Shunsuke Masuo, Takatoshi Arakawa, Chihaya Yamada, Shinya Fushinobu, Naoki Takaya

Journal of Biological Chemistry, Jan, 2024
GH172 difructose dianhydride I synthase: A two-stepped journey via glycosylation and cyclization using QM/MM metadynamics

Bruno Di Geronimo, Toma Kashima, Akihiro Ishiwata, Katsunori Tanaka, Pedro Alejandro Sánchez-Murcia, Kiyotaka Fujita, Shinya Fushinobu, Santiago Alonso-Gil

Dec 26, 2023
【総説：応用糖質科学シンポジウム】<i>Bifidobacterium bifidum</i>由来の硫酸化ムチン糖鎖および血液型抗原分解酵素の構造基盤

Toma Kashima, Toshihiko Katoh, Chihaya Yamada, Takane Katayama, Hisashi Ashida, Shinya Fushinobu

Bulletin of Applied Glycoscience, 13(4) 194-202, Dec 20, 2023
Identification and characterization of endo-α-, exo-α-, and exo-β-D-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria.

Michiko Shimokawa, Akihiro Ishiwata, Toma Kashima, Chiho Nakashima, Jiaman Li, Riku Fukushima, Naomi Sawai, Miku Nakamori, Yuuki Tanaka, Azusa Kudo …

Nature communications, 14(1) 5803-5803, Sep 19, 2023
Novel tetrahydrofolate‐dependent <scp>d</scp>‐serine dehydratase activity of serine hydroxymethyltransferases

Tetsuya Miyamoto, Shinya Fushinobu, Yasuaki Saitoh, Masae Sekine, Masumi Katane, Kumiko Sakai‐Kato, Hiroshi Homma

The FEBS Journal, 291(2) 308-322, Sep 18, 2023
Substrate recognition mode of a glycoside hydrolase family 42 β-galactosidase from Bifidobacterium longum subspecies infantis (BiBga42A) revealed by crystallographic and mutational analyses

Aina Gotoh, Masafumi Hidaka, Haruko Sakurama, Mamoru Nishimoto, Motomitsu Kitaoka, Mikiyasu Sakanaka, Shinya Fushinobu, Takane Katayama

Microbiome Research Reports, 2 20, May, 2023 Peer-reviewed
Synthesis and structural analysis of D-fructofuranosylated compounds for the analysis of GH172 difructose dianhydride I synthase/hydrolase

Akihiro Ishiwata, Toma Kashima, Machika Kaieda, Katsunori Tanaka, Kiyotaka Fujita, Shinya Fushinobu, Yukishige Ito

Pure Appl. Chem., in press, Apr 5, 2023 Peer-reviewedInvited
Identification and structural basis of an enzyme that degrades oligosaccharides in caramel

Kashima, K, Ishiwata, A, Kiyotaka, F, Fushinobu, S

Biophysics and Physicobiology, 20(2) e200017_1-e200017_12, Mar 29, 2023 Peer-reviewedInvited
A bacterial sulfoglycosidase highlights mucin O-glycan breakdown in the gut ecosystem.

Toshihiko Katoh, Chihaya Yamada, Michael D Wallace, Ayako Yoshida, Aina Gotoh, Moe Arai, Takako Maeshibu, Toma Kashima, Arno Hagenbeek, Miriam N Ojima …

Nature chemical biology, Mar 2, 2023

Misc.

275

Discovery and analysis of novel degradation pathway of sulfated mucin O-glycan by gut microbes

加藤紀彦, 山田千早, 伏信進矢, 片山高嶺

生化学, 96(2), 2024
深海微生物叢情報を利用した新規糖質分解酵素の探索~新規酵素群の分子進化と機能の多様性を紐解く~

澄田智美, 平岡聡史, 臼井けい子, 石渡明弘, 伏信進矢, 布浦拓郎

日本生化学会大会(Web), 96th, 2023
Structural Basis of Glycosyl Hydrolases that Degrade Sulfated Mucin Glycans and Blood Group Antigens from Bifidobacterium bifidum

鹿島騰真, 加藤紀彦, 山田千早, 片山高嶺, 芦田久, 伏信進矢, 伏信進矢

応用糖質科学, 13(4), 2023
Synthesis and analysis of the mode of action of GH127/146 β-L-arabinofuranosidase inhibitors

石渡明弘, 藤田清貴, 伏信進矢, 伏信進矢, 田中克典, 田中克典, 伊藤幸成, 伊藤幸成

日本農芸化学会大会講演要旨集(Web), 2023, 2023
Study on analysis of mechanism of β-L-arabinofuranosidase using inhibitor probes

石渡明弘, 成田覚, 成田覚, 藤田清貴, 伏信進矢, 田中克典, 田中克典, 伊藤幸成, 伊藤幸成

日本糖質学会年会要旨集, 42nd, 2023
結核菌が持つ特徴的な細胞壁多糖を分解する新規酵素の構造と機能の解明

福島陸, 石渡明弘, 藤田清貴, 荒川孝俊, 山田千早, 伏信進矢, 伏信進矢

応用糖質科学, 13(3), 2023
深海メタゲノム情報を利用した新規糖質分解酵素群の発見及び構造機能相関の解明

澄田智美, 平岡聡史, 臼井けい子, 石渡明弘, 伏信進矢, 布浦拓郎

応用糖質科学, 13(3), 2023
Microbacterium属由来endo-D-arabinanaseが有するD-arabinan結合性の解析

岩永菜央, 赤井元気, 新地浩之, 隅田泰生, 石渡明弘, 伊藤幸成, 伊藤幸成, 伏信進矢, 北原兼文, 藤田清貴

応用糖質科学, 13(3), 2023
抗酸菌細胞壁中のD-アラビナン糖鎖を分解するGH172α-D-arabinofuranosidaseの構造解析

鹿島騰真, 工藤亜津紗, 石渡明弘, 中島千穂, 藤田清貴, 伏信進矢

応用糖質科学, 13(3), 2023
Preliminary structural study of Rubisco activase from a hydrogen-oxidizing bacterium

廖増威, 荒川孝俊, 荒川孝俊, 新井博之, 新井博之, 亀谷将史, 亀谷将史, 石井正治, 石井正治, 伏信進矢 …

量子ビームサイエンスフェスタ(Web), 2022, 2023
カラメルに含まれるオリゴ糖を分解する酵素の同定と構造基盤

鹿島騰真, 石渡明弘, 藤田清貴, 伏信進矢

生物物理, 62(3) 184-186, Jun, 2022 Peer-reviewedInvited
好熱性水素細菌Hydrogenophilus thermoluteolus TH-1株由来シャペロニンのクライオ電子顕微鏡構造解析

廖増威, 荒川孝俊, 荒川孝俊, 亀谷将史, 亀谷将史, 安達成彦, 守屋俊夫, 池田聡人, 川崎政人, 石井正治 …

日本農芸化学会関東支部講演要旨集(CD-ROM), 2022, 2022
Identification and Structural Basis of an Enzyme Degrading Oligosaccharides in Caramel

鹿島騰真, 石渡明弘, 藤田清貴, 伏信進矢

生物物理(Web), 62(3), 2022
Neutron structural analysis of rhamnose glucuronic acid lyase from Fusarium oxysporum 12S

矢野直峰, 近藤辰哉, 日下勝弘, 山田太郎, 荒川孝俊, 山田千早, 阪本龍司, 伏信進矢

量子ビームサイエンスフェスタ(Web), 2021, 2022
Bifidobacterium dentiumにおけるカラメル化糖の分解メカニズムの解析

海江田麿千加, 奥村公喜, 鹿島騰真, 伏信進矢, 石渡明弘, 伊藤幸成, 伊藤幸成, 北原兼文, 藤田清貴

応用糖質科学, 11(3), 2021
Synthetic study on furanoside derivatives for the bifidobacterial exo-α-D-fructofuranosidase

石渡明弘, 鹿島騰真, 藤田清貴, 伏信進矢, 伏信進矢, 伊藤幸成, 伊藤幸成

日本農芸化学会大会講演要旨集(Web), 2021, 2021
Inhibitor-based studies on β-L-arabinofuranosidase

味木智子, 澤野孝太, 成田覚, 成田覚, 鈴木健裕, 荒川孝俊, 荒川孝俊, 山田千早, 山田千早, 堂前直 …

量子ビームサイエンスフェスタ(Web), 2020, 2021
Mechanistic study on the bifidobacterial exo-α-D-fructofuranosidase

石渡明弘, 藤田清貴, 鹿島騰真, 伏信進矢, 伏信進矢, 田中克典, 田中克典, 田中克典, 伊藤幸成, 伊藤幸成

日本糖質学会年会要旨集, 40th, 2021
フザリウム属由来アラビアガム側鎖末端分解酵素の構造機能解析

近藤辰哉, 近藤辰哉, 吉條美由, 伏信進矢, 阪本龍司

応用糖質科学, 11(3), 2021
カラメル化糖に作用するビフィズス菌の新規exo-α-D-fructofuranosidaseの構造解析

鹿島騰真, 奥村公喜, 荒川孝俊, 山田千早, 石渡明弘, 伊藤幸成, 藤田清貴, 伏信進矢

応用糖質科学, 11(2), 2021

Teaching Experience

Apr, 2017 - Present

Protein Science & Enzymology I (Faculty of Agriculture, The University of Tokyo)
Apr, 2012 - Present

Molecular Aspects of Biocatalysts (GSALS, UTokyo)

Professional Memberships

Research Projects

Control, molecular design, and molecular movie analysis of dynamic catalytic reactions of useful enzymes

Grants-in-Aid for Scientific Research, Grant-in-Aid for Transformative Research Areas (A), Japan Society for the Promotion of Science, Apr, 2024 - Mar, 2029
Prebiotic effects of caramelized sugars based on the degradation mechanism of intestinal bacteria

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2024 - Mar, 2028
Understanding the Intestinal Survival Strategy of Altruistic Bifidobacteria by Human Glycan Binding from the Molecular Structure

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2023 - Mar, 2028
新規酵素を含むレボグルコサン代謝の全容解明とその応用に関する研究

科学研究費助成事業, 基盤研究(B), 日本学術振興会, Apr, 2024 - Mar, 2025

吉田信行, 伏信進矢, 北岡本光
Comprehensive analysis and application of a bacterial Levoglucosan metabolism involving novel enzymes

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2021 - Mar, 2025
Investigation of intestinal lipid metabolism in food allergy

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), Japan Society for the Promotion of Science, Aug, 2020 - Mar, 2025
Enzymatic insight into the microbiota formation in infant guts

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2021 - Mar, 2024
Molecular analysis of mirror-arabinan present in cell wall polysaccharides of Mycobacteria and its degrading enzymes

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2023
Functional analysis and application of sulfoglycosidase from mucin-assimilating bacteria

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2022

Katoh Toshihiko
Structure-function analysis and study on reaction mechanism of microbial cysteine glycosidases

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2019

Fushinobu Shinya
Structural and functional analysis of glycosidases from plants that have a novel active center

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2018

Fushinobu Shinya, FUJITA Kiyotaka, ISHIWATA Akihiro, ITO Yukishige, Beckham Gregg T.
βガラクトシドのプレバイオティクス機能解明にむけたβガラクトシダーゼの構造解析

科学研究費助成事業, 特別研究員奨励費, 日本学術振興会, Jul, 2015 - Mar, 2017

伏信進矢, VIBORG ALEXANDER
Structural analysis of novel bifidobacterial enzymes degrading human and plant glycans

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

FUSHINOBU Shinya, ASHIDA Hisashi, FUJITA Kiyotaka
Elucidation of novel nitrogen metabolisms of microbes

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, 2008 - 2011

SHOUN Hirofumi, SASAKI Yasuyuki, FUSHINOBU Shinya, WAKAGI Takayoshi
Structural and functional analyses of xylan-degrading esterases from koji-mold

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2008 - 2010

KOSEKI Takuya, FUSHINOBU Shinya
Structure determination and functional analysis on novel carbohydrate-active enzymes from fungi and actinomycete

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, 2007 - 2008

FUSHINOBU Shinya
特異な反応・基質認識機構を有する糖質関連酵素の構造機能研究

科学研究費助成事業, 若手研究(B), 日本学術振興会, 2005 - 2006

伏信進矢
構造未知ファミリーに属する糖質分解・転移酵素のX線結晶構造解析

科学研究費助成事業, 若手研究(B), 日本学術振興会, 2003 - 2004

伏信進矢
新規耐熱性β-ガラクトシダーゼのX線結晶構造解析

科学研究費助成事業, 若手研究(B), 日本学術振興会, 2001 - 2002

伏信進矢
Analyses of the crystal structure of 4-α-glucanotransferase and its production mechanism of new cycloamylose

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2000 - 2002

MATSUZAWA Hiroshi, FUSHINOBE Shinya, WAKAGI Takayoshi, KAMIIE Katsuyoshi

Profile Information

Research Interests

Research Areas

Research History

Education

Committee Memberships

Awards

Papers

Misc.

Teaching Experience

Professional Memberships

Research Projects

Menu

Coauthors