論文

査読有り
2006年3月

Role of the tumor suppressor RASSF1A in Mst1-medated apoptosis

CANCER RESEARCH
  • HJ Oh
  • ,
  • KK Lee
  • ,
  • SJ Song
  • ,
  • NS Jin
  • ,
  • MS Song
  • ,
  • JH Lee
  • ,
  • CR Im
  • ,
  • JO Lee
  • ,
  • S Yonehara
  • ,
  • DS Lim

66
5
開始ページ
2562
終了ページ
2569
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1158/0008-5472.CAN-05-2951
出版者・発行元
AMER ASSOC CANCER RESEARCH

Mammalian sterile 20-like kinase 1 (Mst1) is activated by both caspase-mediated cleavage and phosphorylation in response to apoptotic stimuli, including Fas ligation. Here, we examined the possible role of the tumor suppressor RASSF1A in Mst1 activation and Mst1-mediated apoptosis induced by death receptor signaling. Immunoprecipitation and immunofluorescence analyses revealed that Mst1 was associated with RASSF1A in cultured mammalian cells, with both proteins colocalizing to microtubules throughout the cell cycle. Whereas purified recombinant RASSF1A inhibited the kinase activity of purified recombinant Mst1 in vitro, overexpression of RASSF1A increased the kinase activity of Mst1 in intact cells, suggesting that regulation of Mst1 by RASSF1A in vivo involves more than the simple association of the two proteins. Both the activation of Mst1 and the incidence of apoptosis induced by Fas ligation were markedly reduced in cells depleted of RASSF1A by RNA interference and were increased by restoration of RASSF1A expression in RASSF1A-deficient cells. Moreover, the stimulatory effect of RASSF1A overexpression on Fas-induced apoptosis was inhibited by depletion of Mst1. These findings indicate that RASSF1A facilitates Mst1 activation and thereby promotes apoptosis induced by death receptor signaling.

Web of Science ® 被引用回数 : 138

リンク情報
DOI
https://doi.org/10.1158/0008-5472.CAN-05-2951
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000235826400012&DestApp=WOS_CPL

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