TAKAHASHI Shouji

J-GLOBAL         Last updated: Jun 21, 2019 at 19:36
 
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Name
TAKAHASHI Shouji
E-mail
shoutakavos.nagaokaut.ac.jp
Affiliation
Nagaoka University of Technology
Job title
Professor
Research funding number
90324011

Research Areas

 
 

Academic & Professional Experience

 
Apr 1999
 - 
Mar 2000
Researcher, Osaka Science and Technology Center
 

Education

 
 
 - 
Mar 1999
Graduate School, Division of Engineering, Kyoto University
 

Published Papers

 
Draft genome sequence of the yeast Vanrija humicola (formerly Cryptococcus humicola) strain UJ1, a producer of D-aspartate oxidase
Daiki Imanishi, Katsumasa Abe, Yoshio Kera, Shouji Takahashi
6(11) e00068-18   2018   [Refereed]
Taichi Mizobuchi, Risako Nonaka, Motoki Yoshimura, Katsumasa Abe, Shouji Takahashi, Yoshio Kera, Masaru Goto
Acta Crystallographica Section F:Structural Biology Communications   73 651-656   Dec 2017   [Refereed]
© International Union of Crystallography, 2017. Aspartate racemase (AspR) is a pyridoxal 5′-phosphate (PLP)-dependent enzyme that is responsible for d-aspartate biosynthesis in vivo. To the best of our knowledge, this is the first study to report ...
Katsumasa Abe, Naoko Mukai, Yuka Morooka, Takeshi Makino, Kenji Oshima, Shouji Takahashi, Yoshio Kera
Scientific Reports   7    Dec 2017   [Refereed]
© The Author(s) 2017. Sphingobium sp. strain TCM1 can degrade tris(2-chloroethyl) phosphate (TCEP) to inorganic phosphate and 2-chloroethanol. A phosphotriesterase (PTE), phosphodiesterase (PDE) and phosphomonoesterase (PME) are believed to be inv...
Shouji Takahashi, Hiroshi Katanuma, Katsumasa Abe, Yoshio Kera
Applied Microbiology and Biotechnology   101(5) 2153-2162   Mar 2017   [Refereed]
Draft genome sequences of Sphingobium sp. strain TCM1 and Sphingomonas sp. strain TDK1, haloalkyl phosphate flame retardant and plasticizer-degrading bacteria
Yoshio Kera, Katsumasa Abe, Daisuke Kasai, Masao Fukuda, and Shouji Takahashi
Genome Annnouncements   4(4) e00668-16-e00668-1   Jul 2016   [Refereed]
Takahashi S, Shimada K, Nozawa S, Goto M, Abe K, Kera Y.
Journal of Biochemistry   159(3) 371-378   Mar 2016   [Refereed]
Bioengineered   6(4) 237-241   May 2015   [Refereed][Invited]
Shouji Takahashi, Makoto Furukawara, Keishi Omae, Namiho Tadokoro, Yayoi Saito, Katsumasa Abe, Yoshio Kera
Applied Environmental Microbiology   80(23) 7219-7229   Dec 2014   [Refereed]
Shouji Takahashi , Hirotsune Okada, Katsumasa Abe, Yohiso Kera
Applied Biochemistry and Microbiology   50(6) 624-628   Nov 2014   [Refereed]
Yayoi Saito, Shouji Takahashi , Mieko Kobayashi, Katsumasa Abe, Yoshio Kera
Annals of Microbiology   64(3) 1167-1177   Sep 2014   [Refereed]
Abe K,Yoshida Y,Suzuki Y,Mori J,Doi Y,Takahashi S,Kera Y
Applied and Environmental Microbiology   80(18) 5866-5873   Sep 2014   [Refereed]
Kato S, Ikuta T, Hemmi H, Takahashi S, Kera Y, Yoshimura T.
Bioscience, Biotechnology, and Biochemistry   76(11) 2150-2151   Nov 2012   [Refereed]
Biodegradation of Persistent Chlorinated Organophosphorus Flame Retardants by Microorganisms Newly Isolated from Soil
Kera, Y., Takahashi, S., Abe, K.
Transactions on GIGAKU   1(1) 01011/1-4   Jul 2012   [Refereed]
Takahashi, S., Matsumoto, S., Maruyama, K., Wakaizumi, A., Abe, K., Kera, Y. and Yamada, R.
Journal of Molecular Catalysis B:Enzymatic   61(3) 235-240   Sep 2009   [Refereed]
Sato, R., Matsumoto, T., Hidaka, N., Imai, Y., Abe, K., Takahashi, S., Yamada, R. and Kera,Y.
Protein Expression and Purification   64(2) 205-212   Apr 2009   [Refereed]
Ryo Hei Yamada, Ryo Hei Yamada, Yoshio Kera, Shouji Takahashi, Takashi Watanabe, Kimihiko Shibata
D-Amino Acids: A New Frontier in Amino Acid and Protein Research - Practical Methods and Protocols   25-32   Dec 2007   [Refereed]
Sato, R., Mitani, K., Matsumoto, T., Takahashi, S., Yamada, R. and Kera, Y.
Japanese Journal of Environmental Toxicology   10(1) 31-38   Oct 2007   [Refereed]
Ryo Hei Yamada, Yoshio Kera, Shouji Takahashi
Chemical Record   6 259-266   Dec 2006   [Refereed]
D-Aspartate is one of a few D-amino acids that attracted attention at an early date, since it was detected in various tissues of mammals as a protein component. The occurrence of free D-aspartate in nature was recognized a little later, and raised...
Katsumasa Abe, Shouji Takahashi, Yoshinori Muroki, Yoshio Kera, Ryo Hei Yamada
Journal of Biochemistry   139 235-244   Feb 2006   [Refereed]
D-Aspartate is present at high concentrations in the tissues of Scapharca broughtonii, and its production depends on aspartate racemase. This enzyme is the first aspartate racemase purified from animal tissues and unique in its pyridoxal 5′-phosph...
Shouji Takahashi, Taiki Kakuichi, Kazuhito Fujii, Yoshio Kera, Ryo Hei Yamada
Yeast   22 1203-1212   Nov 2005   [Refereed]
The physiological role of D-aspartate oxidase (ChDASPO) in the yeast Cryptococcus humicola was analysed through the growth characteristics of a ChDASPO gene-disrupted strain (daspoΔ) and the expression profile of ChDASPO on various combinations of...
T. Watanabe, T. Watanabe, K. Shibata, Y. Kera, S. Takahashi, R. Yamada
Amino Acids   28 291-296   May 2005   [Refereed]
Blood shell, Scapharca broughtonii, contains large quantities of free D-aspartate comparable to free L-aspartate in its tissues. When the shell was reared in hypoxic seawater, D-aspartate as well as L-aspartate in the foot muscle decreased rapidly...
Kenji Oshima, Hiromichi Nakajima, Shouji Takahashi, Yoshio Kera, Masato Shimomura, Shinnosuke Miyauchi
Sensors and Actuators, B: Chemical   105 473-478   Mar 2005   [Refereed]
Anti-vitellogenin antibody was immobilized on a quartz crystal oscillator and applied to a quartz crystal microbalance (QCM) for detection of plasma vitellogenin (Vtg) of fish. The QCM exhibited a significant decrease in frequency responding to Vt...
Shouji Takahashi, Toshiyuki Takahashi, Yoshio Kera, Ryuji Matsunaga, Hiroo Shibuya, Ryo Hei Yamada
Journal of Biochemistry   135 533-540   Apr 2004   [Refereed]
The D-aspartate oxidase (DDO) from the yeast Cryptococcus humicola UJ1 (ChDDO) is highly specific to D-aspartate. The gene encoding ChDDO was cloned and expressed in Escherichia coli. Sequence analysis of the ChDDO gene showed that an open reading...
Kimihiko Shibata, Kimihiko Shibata, Takashi Watanabe, Hiroyuki Yoshikawa, Katsumasa Abe, Shouji Takahashi, Yoshio Kera, Ryo Hei Yamada
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   134 713-719   Apr 2003   [Refereed]
The activity of D-aspartate racemase purified from Scapharca broughtonii has been found to depend markedly on some nucleotides. Purine nucleoside monophosphates enhanced the enzyme activity, which was, on the contrary, lowered by purine nucleoside...
Kimihiko Shibata, Kimihiko Shibata, Takashi Watanabe, Hiroyuki Yoshikawa, Katsumasa Abe, Shouji Takahashi, Yoshio Kera, Ryo Hei Yamada
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   134 307-314   Feb 2003   [Refereed]
High concentrations of D-aspartate occur in blood shell Scapharca broughtonii (Mollusca) tissues. We purified aspartate racemase from the foot muscle of the bivalve to electrophoretic homogeneity. The molecular mass shown by sodium dodecyl sulfate...
Atsuko Tarui, Kimihiko Shibata, Kimihiko Shibata, Shouji Takahashi, Yoshio Kera, Toratane Munegumi, Ryo H. Yamada
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   134 79-87   Jan 2003   [Refereed]
The presence of N-methyl-D-glutamate (NMDG) and N-methyl-L-glutamate (NMLG) has been demonstrated in the tissues of Scapharca broughtonii, which are known to contain N-methyl-D-aspartate (NMDA). To our knowledge, this is the first report on the na...
Shouji Takahashi, Ryuji Matsunaga, Yoshio Kera, Ryo Hei Yamada
Journal of Bioscience and Bioengineering   96 23-31   Jan 2003   [Refereed]
A transformation system for a yeast, Cryptococcus humicolus, was constructed. As a selectable marker, the URA3 gene encoding orotidine-5′-phosphate decarboxylase (OMPdecase) was isolated from a C. humicolus genomic DNA library, and the equivalent ...
Yasuya Fujita, Shouji Takahashi, Mitsuyoshi Ueda, Atsuo Tanaka, Hirofumi Okada, Yasushi Morikawa, Takashi Kawaguchi, Motoo Arai, Hideki Fukuda, Akihiko Kondo
Applied and Environmental Microbiology   68 5136-5141   Oct 2002   [Refereed]
For direct and efficient ethanol production from cellulosic materials, we constructed a novel cellulose-degrading yeast strain by genetically codisplaying two cellulolytic enzymes on the cell surface of Saccharomyces cerevisiae. By using a cell su...
Takeshi Matsumoto, Shouji Takahashi, Mitsuyoshi Ueda, Atsuo Tanaka, Hideki Fukuda, Akihiko Kondo
Journal of Molecular Catalysis - B Enzymatic   17 143-149   Jun 2002   [Refereed]
Yeast whole cell biocatalysts, which intracellularly overproduced a recombinant lipase with a pro-sequence from Rhizopus oryzae IFO4697 (rProROL) were constructed, and the content of active lipase in Saccharomyces cerevisiae cells was maximized by...
Mitsuyoshi Ueda, Shouji Takahashi, Motohisa Washida, Seizaburo Shiraga, Atsuo Tanaka
Journal of Molecular Catalysis - B Enzymatic   17 113-124   Jun 2002   [Refereed]
The extracellular production of active Rhizopus oryzae lipase (ROL) was carried out by the expression of the ProROL gene encoding a pro-form of ROL (ProROL) using prepro-α-factor in Saccharomyces cerevisiae. Two forms of recombinant ROL (rROL), rP...
Seizaburo Shiraga, Mitsuyoshi Ueda, Shouji Takahashi, Atsuo Tanaka
Journal of Molecular Catalysis - B Enzymatic   17 167-173   Jun 2002   [Refereed]
The lid domain of lipase is an interesting portion which has a large effect on the substrate specificity of the enzyme. To investigate the relationship between the amino acid sequence of the lid domain of Rhizopus oryzae lipase (ROL) and its subst...
A. Kondo, H. Shigechi, M. Abe, K. Uyama, T. Matsumoto, S. Takahashi, M. Ueda, A. Tanaka, M. Kishimoto, H. Fukuda
Applied Microbiology and Biotechnology   58 291-296   Mar 2002   [Refereed]
A Strain of host yeast YF207, which is a tryptophan auxotroph and shows strong flocculation ability, was obtained from Saccharomyces diastaticus ATCC60712 and S. cerevisiae W303-1B by tetrad analysis. The plasmid pGA11, which is a multicopy plasmi...
Kimihiko Shibata, Atsuko Tarui, Natsumi Todoroki, Shinjiro Kawamoto, Shouji Takahashi, Yoshio Kera, Ryo H. Yamada
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   130 493-500   Nov 2001   [Refereed]
The presence of N-methyl-L-aspartate (NMLA) was demonstrated in bivalves, Corbicula sandai and Tapes japonica. To our knowledge, this is the first report on the occurrence of NMLA in animal tissues. NMLA in bivalve tissues was identified according...
M. Washida, S. Takahashi, M. Ueda, A. Tanaka
Applied Microbiology and Biotechnology   56 681-686   Sep 2001   [Refereed]
We have constructed a Saccharomyces cerevisiae strain displaying an active lipase on the cell surface by cell surface engineering. The gene encoding Rhizopus oryzae lipase (ROL) was fused with the genes encoding the pre-α-factor leader sequence an...
S. Takahashi, M. Ueda, A. Tanaka
Applied Microbiology and Biotechnology   55 454-462   May 2001   [Refereed]
The role of the prosequence of Rhizopus oryzae lipase (ROL) with a preprosequence was analyzed by an expression system using Saccharomyces cerevisiae. When the mature portion of ROL (mROL) fused to the pre-α-factor leader sequence was expressed, s...
T. Matsumoto, S. Takahashi, M. Kaieda, M. Ueda, A. Tanaka, H. Fukuda, A. Kondo
Applied Microbiology and Biotechnology   57 515-520   Jan 2001   [Refereed]
Yeast whole-cell biocatalysts for lipase-catalyzed reactions were constructed by intracellularly overproducing Rhizopus oryzae lipase (ROL) in Saccharomyces cerevisiae MT8-1. The gene encoding lipase from R. oryzae IFO4697 was cloned, and intracel...
Shouji Takahashi, Mitsuyoshi Ueda, Atsuo Tanaka
Journal of Molecular Catalysis - B Enzymatic   10 233-240   Oct 2000   [Refereed]
The Saccharomyces cerevisiae Kex2p is a membrane-bound endoprotease and cleaves at a C-terminal site of Lys-Arg of the α-factor precursor in late Golgi compartment. The complete cleavage of a secreted recombinant Rhizopus oryzae lipase (ROL) precu...
S. Takahashi, M. Ueda, A. Tanaka
Applied Microbiology and Biotechnology   52 534-540   Nov 1999   [Refereed]
A mixture of rProROL having the full-length prosequence (97 amino acids) for a recombinant lipase of Rhizopus oryzae (rROL) and r28ROL having 28 amino acids of the same prosequence has been produced as active forms by Saccharomyces cerevisiae [Tak...
Shouji Takahashi, Mitsuyoshi Ueda, Haruyuki Atomi, Hans D. Beer, Uwe T. Bornscheuer, Rolf D. Schmid, Atsuo Tanaka
Journal of Fermentation and Bioengineering   86 164-168   Jan 1998   [Refereed]
The gene encoding Rhizopus oryzae lipase (ROL) was successfully expressed, and extracellularly active ROL was produced by Saccharomyces cerevisiae. The genes encoding the mature region (mROL) and the mROL having the pro sequence (ProROL) were fuse...

Books etc

 
The Handbook of Microbial Metabolism of Amino Acids
Takahashi, S., Shibata, K., Abe, K. and Kera, Y. (Part:Joint Work, Functions and metabolisms of D-amino acids in microorganisms)
CABI publishing   Apr 2017   
Environmental Biotechnology - New Approaches and Prospective Applications
Takahashi, S., Abe, K. and Kera, Y. (Part:Joint Work, Microbial degradation of persistent organophosphorus flame retardants)
Intech   Feb 2013   
Microbial Bioremediation of Non-metals
Abe, K., Takahashi, S., Kera, Y (Part:Joint Work, Organophosphrus compound-degradaing bacteria and enzyme, and their application)
Caister Academic Press   Jul 2011   ISBN:978-1-904455-83-7
D-Amino Acids: Practical Methods and Protocols, Volume 1: Analytical Methods for D-Amino Acids
Yamada, R., Kera, Y., Takahashi, S., Abe, K. (Part:Joint Work, Determination of acidic D-amino acids and their N-methyl derivatives in bivalves and other mollusks)
Nova Science Publishers Inc.   Jan 2009   
D-Amino Acids: New Frontier in Amino Acid and Protein Research.
Yamada, R., Kera, Y., Takahashi, S., Watanabe, T. and Shibata, K. (Part:Joint Work, Determination of acidic D-amino acids and their N-methyl derivatives in bivalves and other mollusks)
Nova Sciences Publishers Inc.   Jan 2007   ISBN:1-60021-075-9
Focus on Biotechnology, Vol. 2. (Applied Microbiology)
Ueda, M., Murai, T., Takahashi, S., Washida, M. and Tanaka, A. (Part:Joint Work, Molecular breeding of arming yeasts with hydrolytic enzymes by Cell Surface Engineering)
Kluwer Academic Publishers   Jan 2001   

Conference Activities & Talks

 
D-Amino acid oxidase and D-aspartate oxidase of thermophilic fungi
Takahashi S, Osugi K, Shimekake Y, Abe K, Kera Y.
The 3rd International Conference of D-Amino Acid Research   12 Jul 2017   
Crystal Structure of Yeast D-Aspartate Oxidase
The 4th Joint Symposium CU-NUT   21 Oct 2015   Chulalongkorn university and Nagaoka university of technology
Physiological role of haloalkylphosphorus hydrolase from Sphingobium sp. Strain TCM1
Nishigori, Y., Majima, R., Abe, K., Takahashi, S. and Kera, Y.
The 4th International GIGAKU Conference in Nagaoka   20 Jun 2015   
Identification and characterization of putative alkaline phosphatase genes of a persistent frame retardant-degrading Bacterium
Akimoto,M., Abe, K., Takahashi, S. and Kera, Y.
The 4th International GIGAKU Conference in Nagaoka   20 Jun 2015   
Physiological role of haloalkylphosphorus hydrolase from Sphingomonas sp. strain TDK1
The 4th International GIGAKU Conference in Nagaoka   20 Jun 2015   
THE ROLE OF HISTIDINE 56 IN THE SUBSTRATE SPECIFICITY OF THE YEAST Cryptococcus humicola D-ASPARTATE OXIDASE
The 2nd international conference of D-amino acid research   2 Sep 2014   D-アミノ酸学会
CHARACTERIZATION OF D-AMINO ACID OXIDASE OF THE THERMOPHILIC BACTERIUM Rubrobacter xylanophilus
The 2nd international conference of D-amino acid research   2 Sep 2014   D-アミノ酸学会
THE THREE-DIMENSIONAL STRUCTURE OF D-ASPARTATE?OXIDASE OF THE YEAST Cryptococcus humicola [Invited]
The 2nd international conference of D-amino acid research   2 Sep 2014   D-アミノ酸学会
Mutation analysis of PLP-dependent aspartate racemace from blood shell
The 3rd international GIGAKU conference in Nagaoka (IGCN2014)   21 Jun 2014   Nagaoka University of Technology
Identification and characterization of putative alkaline phosphatase gene of a persistent flame retardant-degrading bacterium
The 3rd international GIGAKU conference in Nagaoka (IGCN2014)   21 Jun 2014   Nagaoka University of Technology
Cloning of the gene for tris(1,3-dichloro-2-propyl)phosphate-degrading enzyme from Sphingomonas sp. TDK1
The 3rd international GIGAKU conference in Nagaoka (IGCN2014)   21 Jun 2014   Nagaoka University of Technology
Analysis of substrates recognition mechanism of a yeast D-asparate oxidase
The 3rd international GIGAKU conference in Nagaoka (IGCN2014)   21 Jun 2014   Nagaoka University of Technology
Development of a gene manipulation system of oleaginous yeast Lipomyces starkey
The 3rd international GIGAKU conference in Nagaoka (IGCN2014)   21 Jun 2014   Nagaoka University of Technology
Cloning of the gene for tris(2-chloroethyl)phosphate-degrading enzyme from Sphingobium sp. TCM1.
The 3rd international GIGAKU conference in Nagaoka (IGCN2014)   21 Jun 2014   Nagaoka University of Technology
Characterization of D-amino acid oxidase of the thermophilic bacterium Rubrobacter xylanophilus
The 3rd international GIGAKU conference in Nagaoka (IGCN2014)   21 Jun 2014   Nagaoka University of Technology
Analysis of substrates recognition mechanism of a yeast D-asparate oxidase
The 3rd international GIGAKU conference in Nagaoka (IGCN2014)   21 Jun 2014   Nagaoka University of Technology
Development of an autonomously replicating vector for the yeast Cryptococcus humicola using a telomer-like sequence
14 Dec 2007   
Purification of recombinant carp acetylcholinesterase
13 Dec 2007   
Purification and characterization from Koi carp muscle
13th INTERNATIONAL SYMPOSIUM ON TOXICITY ASSESSMENT   21 Aug 2007   
Isolation and characterization of tris(1,3-dichloro-2-propyl) phosphate degrading bacterium, Sphingomonas sp. strain TDK1
The 8th internationla symposium on Global Renaissance by Green Energy Revoluiton   22 Jan 2007   
Production of recombinant carp acetylcholinesterase by Escherichia coli and its purification
The 8th international symposium on Global Renaissance by Green Energy Revoluiton   22 Jan 2007   
Development of an autonomously replicating vector for the yeast Cryptococcus humicola using a telomere-like sequence
The 8th international symposium on Global Renaissance by Green Energy Revoluiton   22 Jan 2007   
Purificaiton and characterization of recombinant carp acetylcholinesterase produced by the methylotrophic yeast Pichia pastoris
The 8th international symposium on Global Renaissance by Green Energy Revoluiton   22 Jan 2007   
Tris(2-chloroethyl) phosphate-degrading chracteristics of Sphingobium sp. TCM1 and purificaiton of athe resposible enzyme
The 8th international symposium on Global Renaissance by Green Energy Revoluiton   22 Jan 2007   
Development of purification method for tris(1,3-dichloro-2-propyl) phosphate-degrading enzyme of Sphingomonas sp. TDK1
The 8th international symposium on Global Renaissance by Green Energy Revoluiton   22 Jan 2007   
Crystal structure of aspartate racemase from the blood shell Scapharca broughtonii
The 8th international symposium on Global Renaissance by Green Energy Revoluiton   22 Jan 2007   
D-Aspartate and aspartate racemase activity in starfish
The 8th international symposium on Global Renaissance by Green Energy Revoluiton   22 Jan 2007   

Research Grants & Projects

 
Engineering of industrially useful yeasts
Project Year: Apr 2009   
Functional analysis and applications of vitamin-containing enzymes
The Other Research Programs
Project Year: Apr 2000   
Microbial degradation of environmental pollutants
The Other Research Programs
Project Year: Apr 2000