Arabidopsis thaliana exhibits durable 'non-host' resistance against the hemibiotrophic fungal pathogen Colletotrichum tropicale that infects mulberry plants. Arabidopsis non-host resistance comprises two layers of defense: preinvasive and postinvasive resistance. The EDR1 protein kinase contributes to Arabidopsis preinvasive resistance against C. tropicale by inducing the expression of plant defensin (PDF) genes. Here we report that the expressions of multiple PDF genes were strongly induced in Arabidopsis upon invasion by C. tropicale. Invasion by a necrotrophic pathogen, Alternaria brassicicola, also induced PDF expression. Importantly, PDF expression triggered upon invasion by both pathogens was inhibited in edr1 mutants, indicating the requirement of EDR1 for PDF expression in postinvasive resistance by Arabidopsis. Analysis of ora59 mutants also revealed that this gene is critical for induced PDF expression following pathogen invasion. Furthermore, inoculation assays of A. brassicicola indicated that ORA59 is involved in postinvasive resistance against the pathogen, suggesting invasion-triggered PDF expression contributes to postinvasive resistance in Arabidopsis.