2009年12月
Bioorganic synthesis of a recombinant HIV-1 fusion inhibitor, SC35EK, with an N-terminal pyroglutamate capping group
BIOORGANIC & MEDICINAL CHEMISTRY
- 巻
- 17
- 号
- 23
- 開始ページ
- 7964
- 終了ページ
- 7970
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmc.2009.10.017
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
The bioorganic synthesis of an end-capped anti-HIV peptide from a recombinant protein was investigated. Cyanogen bromide-mediated cleavage of two Met-Gln sites across the target anti-HIV sequence generated an HIV-1 fusion inhibitor (SC35EK) analog bearing an N-terminal pyroglutamate (pGlu) residue and a C-terminal homoserine lactone (Hsl) residue. The end-capped peptide, pGlu-SC35EK-Hsl, had similar bioactivity and biophysical properties to the parent peptide, and an improved resistance to peptidase-mediated degradation was observed compared with the non-end-capped peptide obtained using standard recombinant technology. (c) 2009 Elsevier Ltd. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.bmc.2009.10.017
- CiNii Articles
- http://ci.nii.ac.jp/naid/120002098852
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19864148
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000271521000014&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1016/j.bmc.2009.10.017
- ISSN : 0968-0896
- CiNii Articles ID : 120002098852
- PubMed ID : 19864148
- Web of Science ID : WOS:000271521000014