2013年5月
Enhanced Plasmid DNA Transfer into Tumor Cells by Nanoparticle Composed of Cholesteryl Triamine and Diamine
BIOLOGICAL & PHARMACEUTICAL BULLETIN
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- 巻
- 36
- 号
- 5
- 開始ページ
- 856
- 終了ページ
- 860
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1248/bpb.b12-01062
- 出版者・発行元
- PHARMACEUTICAL SOC JAPAN
Previously, we prepared cationic nanoparticles (NP and NP-N) composed of cholesteryl diamine (OH-Chol, (3S)-N-(2-(2-hydroxyethylamino)ethyl)cholesteryl-3-carboxamide) and cholesteryl triamine (OH-N-Chol, (3S)-N-(2-(2-(2-hydroxyethylamino)ethylamino)ethyl)cholesteryl-3-carboxamide), respectively, with Tween 80 for small interfering RNA (siRNA) delivery into tumor cells. I this study, we prepared NP-0.25N composed of OH-Chol and OH-N-Chol at a molar ratio of 3/1 with Tween 80, and evaluated the transfection efficiency of plasmid DNA (pDNA) into tumor cells. NP-N exhibited lower transfection activity than NP; however, NP-0.25N showed higher transfection activity than both NP and NP-N in various tumor cells. NP-0.25N increased the amount of internalized pDNA by increased cellular association, and improved the escape from endosomes after clathrin-mediated endocytosis. The results of the experiments suggested that cholesteryl triamine may have potential as a helper lipid to increase the transfection for pDNA delivery by cationic cholesterol-based nanoparticles.
- リンク情報
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- DOI
- https://doi.org/10.1248/bpb.b12-01062
- CiNii Articles
- http://ci.nii.ac.jp/naid/130003361415
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/23649342
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000318331400026&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1248/bpb.b12-01062
- ISSN : 0918-6158
- CiNii Articles ID : 130003361415
- PubMed ID : 23649342
- Web of Science ID : WOS:000318331400026