J-GLOBAL         Last updated: Sep 10, 2019 at 16:42
Kumamoto University
Institute of Resource Development and Analysis, Division of Reproductive Engineering, Center for Animal Resources and Development (CARD)
Job title
Doctor of Medicine, Master of Agriculture

Research Interests


Research Areas



Graduate School, Division of Animal Sciences, Kitasato University
Faculty of Animal Science, Kitasato University

Published Papers

Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation
Nakagawa Y, Sakuma T, Takeo T, Nakagata N, Yamamoto T
Experimental Animals   67(4) 535-543   Nov 2018   [Refereed]
Lowering effect of dimethyl-α-cyclodextrin on GM1-ganglioside accumulation in GM1-gangliosidosis model cells and in brain of β-galactosidase-knockout mice
Yuki Maeda, Keiichi Motoyama, Taishi Higashi, Risako Onodera, Toru Takeo, Naomi Nakagata, Yuki Kurauchi, Hiroshi Katsuki, Yoichi Ishitsuka, Yuki Kondo, Tetsumi Irie, Takumi Era, Hidetoshi Arima
Journal of Inclusion Phenomena and Macrocyclic Chemistry   93(1) 53-66   Aug 2018   [Refereed]
Mouse Sperm Cryopreservation Using Cryoprotectant Containing l-Glutamine.
Cold Spring Harb Protoc      2018   [Refereed]
Androgen Regulates Dimorphic F-Actin Assemblies in the Genital Organogenesis.
Liu L, Suzuki K, Chun E, Murashima A, Sato Y, Nakagata N, Fujimori T, Yonemura S, He W, Yamada G.
11(4) 190-202   Dec 2017   [Refereed]
Zinc Deficiency via a Splice Switch in Zinc Importer ZIP2/SLC39A2 Causes Cystic Fibrosis-Associated MUC5AC Hypersecretion in Airway Epithelial Cells.
Kamei S, Fujikawa H, Nohara H, Ueno-Shuto K, Maruta K, Nakashima R, Kawakami T, Matsumoto C, Sakaguchi Y, Ono T, Suico MA, Boucher RC, Gruenert DC, Takeo T, Nakagata N, Li JD, Kai H, Shuto T
EBioMedicine       Dec 2017   [Refereed]


High survival rate of untertilized mouse oocytes after vitrification
Journal of Reproduction and Fertility   87 479-483   1989
High fertilizing ability of mouse snermatozoa diluted slowly after cryopreservation
Theriogenology   37 1283-1291   1992
Production of normal young following transfer of mouse embryos obtained by in nitro fertilization between cryopreserved gametes
Journal of Reproduction and Fertility   99 77-80   1993
Cryopreservation of wild mice spermatozoa
Theriogenology   43 635-643   1995
Use of cryopreservation techniques of embryos and spermatozoa for production of transgenic(Tg)mice and for maintenance of Tg mouse lines.
Laboratory animal science   46 236-238   1996

Books etc

Efficient Gene Transduction Technique by microbubble-enhances sonoporation for embryology.
Humana Press   2005   

Conference Activities & Talks

Cold transport of mouse sperm
Koharu Kirikihira, Hidetaka Yoshimoto, Tetsumi Irie, Toru Takeo, Naomi Nakagata
2017 AMMRA & AMPC Meeting   26 Aug 2017   
Cryopreservation of mouse oocytes derived from ultrasuperovulation
Chihiro Sugahara, Ayumi Mukunoki, Toru Takeo, Naomi Nakagata
2017 AMMRA & AMPC Meeting   26 Aug 2017   
Cold transport of two-cell mouse embryos
Kana Tamura, Yuka Horikoshi, Kazuki Shirakado, Toru Takeo, Naomi Nakagata
2017 AMMRA & AMPC Meeting   26 Aug 2017   
Creating a platform for mouse research using reproductive technology [Invited]
Toru Takeo and Naomi Nakagata
2017 AMMRA & AMPC Meeting   26 Aug 2017   
Shipping mice vs. frozen or refrigerated embryos/sperm
Toru Takeo, Hidetaka Yoshimoto, Naomi Nakagata
CARD-IP Mouse Embryo and Sperm Cryopreservation Workshop   26 Oct 2016   
A strategy to accelerate cardiovascular research by using advanced reproductive technology of “Mouse Resource Bank”
Toru Takeo and Naomi Nakagata
New Era of Angiogenesis Research   7 Jul 2016   
Effect of cholesterol efflux from plasma membrane on the fertility of cold-stored mouse sperm
20 May 2016   
Efficient animal production using oocyte cryopreservation derived from ultra-superovulation technique in mice
Ayumi Mukunoki, Toru Takeo, Naomi Nakagata
20 May 2016   
Application of inhibin antiserum for superovulation in mice
Toru Takeo, Ayumi Mukunoki, Naomi Nakagata
The 2016 AMMRA & AMPC meeting   20 May 2016   
A novel superovulation technique using inhibin antiserum and equine chorionic gonadotropin in C57BL/6 mice
Toru Takeo, Ayumi Mukunoki, Naomi Nakagata
13th Transgenic Technology Meeting   22 Mar 2016   

Research Grants & Projects

Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2003 - 2004    Investigator(s): Naomi NAKAGATA
The developed system is designed to consolidate information necessary for mouse banking system including :1.basic information, such as mouse strain name, background characteristics, and depositor information2.information before embryos are produce...
Study on Cryopreservation of embryo and gamete