MISC

2002年4月

Apoptosis induction by lectin isolated from the mushroom Boletopsis leucomelas in U937 cells

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Y Koyama
  • ,
  • Y Katsuno
  • ,
  • N Miyoshi
  • ,
  • S Hayakawa
  • ,
  • T Mita
  • ,
  • H Muto
  • ,
  • S Isemura
  • ,
  • Y Aoyagi
  • ,
  • M Isemura

66
4
開始ページ
784
終了ページ
789
記述言語
英語
掲載種別
DOI
10.1271/bbb.66.784
出版者・発行元
TAYLOR & FRANCIS LTD

A 15-kDa lectin was isolated from the edible mushroom Kurokawa by affinity chromatography using N,N'-diacetylehitobiose-Sepharose 4B. The results of microsequencing analysis indicated that the lectin has a partial amino acid sequence similar to the mushroom lectin, Agaricus bisporus agglutinin (ABA). We found that the Kurokawa lectin inhibited proliferation of human monoblastic leukemia U937 cells dose-dependently. Several lines of evidence indicated that this inhibition was due to its apoptosis induction. We observed that the lectin induced apoptotic bodies formation, chromatin condensation, and DNA ladder formation, features of apoptosis. The DNA ladder formation was inhibited by a general inhibitor of caspases, which are known to play essential roles in apoptosis. In contrast. ABA did not have cell growth-inhibiting or apoptosis-inducing activities. Thus, the Kurokawa lectin is the first mushroom lectin with apoptosis-inducing activity.

Web of Science ® 被引用回数 : 34

リンク情報
DOI
https://doi.org/10.1271/bbb.66.784
CiNii Articles
http://ci.nii.ac.jp/naid/10020775362
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12036051
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000175360500013&DestApp=WOS_CPL

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