Noriyuki Doukyu

Profile Information

Affiliation: Associate Professor, Faculty of Life Sciences, Toyo University

Degree: (BLANK)(Tokyo Institute of Technology)

Researcher number: 60302957
J-GLOBAL ID: 200901020061507727
researchmap Member ID: 1000279217

Research Interests

Research Areas

Research History

1998 - 2003

Bioscience and Biotechnology, Tokyo Institute of Technology

Education

Papers

Expression and characterization of cholesterol oxidase with high thermal and pH stability from Janthinobacterium agaricidamnosum.

Noriyuki Doukyu, Yuuki Ikehata, Taichi Sasaki

Preparative biochemistry & biotechnology, 53(3) 331-339, 2023
Improving E. coli organic solvent tolerance by 1,4-dihydroxy-2-naphthoic acid.

Noriyuki Doukyu, Hideyuki Fujisawa, Ryota Saito

Bioscience, biotechnology, and biochemistry, 86(8) 1128-1135, Jul 22, 2022
Improving the organic solvent tolerance of Escherichia coli with vanillin, and the involvement of an AcrAB-TolC efflux pump in vanillin tolerance

Yuuki Ikehata, Noriyuki Doukyu

Journal of Bioscience and Bioengineering, 133(4) 347-352, Apr, 2022
Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli

Noriyuki Doukyu, Katsuya Taguchi

AMB Express, 11(1) 97-97, Dec, 2021
Cholesterol oxidase from Rhodococcus erythropolis with high specificity toward β-cholestanol and pytosterols

Noriyuki Doukyu, Makoto Ishikawa

PLOS ONE, 15(10) e0241126-e0241126, Oct 26, 2020
Production of styrene oxide from styrene by a recombinant Escherichia coli with enhanced AcrAB-TolC efflux pump level in an aqueous-organic solvent two-phase system

Noriyuki Doukyu, Shinichiro Iida

Bioscience, Biotechnology, and Biochemistry, 84(7) 1513-1520, Jul 2, 2020 Peer-reviewed
Cholesterol oxidase with high catalytic activity from Pseudomonas aeruginosa: Screening, molecular genetic analysis, expression and characterization

Noriyuki Doukyu, Shyou Nihei

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 120(1) 24-30, Jul, 2015 Peer-reviewed
Improvement of organic solvent tolerance by disruption of the ion gene in Escherichia coli

Rei Watanabe, Noriyuki Doukyu

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 118(2) 139-144, Aug, 2014 Peer-reviewed
Hyper-activation of foldase-dependent lipase with lipase-specific foldase

Hiroyasu Ogino, Sosuke Inoue, Masahiro Yasuda, Noriyuki Doukyu

JOURNAL OF BIOTECHNOLOGY, 166(1-2) 20-24, Jun, 2013 Peer-reviewed
Halarchaeum salinum sp nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt

Yuto Yamauchi, Hiroaki Minegishi, Akinobu Echigo, Yasuhiro Shimane, Hirokazu Shimoshige, Masahiro Kamekura, Takashi Itoh, Noriyuki Doukyu, Akira Inoue, Ron Usami

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 63 1138-1142, Mar, 2013 Peer-reviewed
Halarchaeum rubridurum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt samples

Yuto Yamauchi, Hiroaki Minegishi, Akinobu Echigo, Yasuhiro Shimane, Masahiro Kamekura, Takashi Itoh, Moriya Ohkuma, Noriyuki Doukyu, Akira Inoue, Ron Usami

International Journal of Systematic and Evolutionary Microbiology, 63(9) 3143-3147, 2013 Peer-reviewed
Novel cholesterol oxidases which were found in the studies of applications of organic solvent-tolerant bacteria.

道久則之

J. Jpn. Soc. Extremophiles, 10 13-16, Mar, 2012
Improvement in organic solvent tolerance by double disruptions of proV and marR genes in Escherichia coli

N. Doukyu, K. Ishikawa, R. Watanabe, H. Ogino

JOURNAL OF APPLIED MICROBIOLOGY, 112(3) 464-474, Mar, 2012 Peer-reviewed
Contributions of mutations in acrR and marR genes to organic solvent tolerance in Escherichia coli

Rei Watanabe, Noriyuki Doukyu

AMB EXPRESS, 2 58, 2012 Peer-reviewed
疎水性有機溶媒に対する大腸菌の耐性機構

道久則之

生物工学会誌, 89(5) 239-241, Jun, 2011
Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp DS-1

Martin Sagermann, Akashi Ohtaki, Keith Newton, Noriyuki Doukyu

JOURNAL OF STRUCTURAL BIOLOGY, 170(1) 32-40, Apr, 2010 Peer-reviewed
Enhancement of the aspartame precursor synthetic activity of an organic solvent-stable protease

Hiroyasu Ogino, Shotaro Tsuchiyama, Masahiro Yasuda, Noriyuki Doukyu

PROTEIN ENGINEERING DESIGN & SELECTION, 23(3) 147-152, Mar, 2010
Organic solvent-tolerant enzymes

Noriyuki Doukyu, Hiroyasu Ogino

BIOCHEMICAL ENGINEERING JOURNAL, 48(3) 270-282, Feb, 2010 Peer-reviewed
Characteristics and biotechnological applications of microbial cholesterol oxidases

Noriyuki Doukyu

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 83(5) 825-837, Jul, 2009
Cloning, sequence analysis, and expression of a gene encoding Chromobacterium sp DS-1 cholesterol oxidase

Noriyuki Doukyu, Kanpei Shibata, Hiroyasu Ogino, Martin Sagermann

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(3) 479-490, Mar, 2009

Misc.

Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays

K Shimizu, S Hayashi, T Kako, M Suzuki, N Tsukagoshi, N Doukyu, T Kobayashi, H Honda

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(2) 1093-1096, Feb, 2005
Time-course data analysis of gene expression profiles reveals purR regulon concerns in organic solvent tolerance in Escherichia coli

Kazunori Shimizu, Shuhei Hayashi, Noriyuki Doukyu, Takeshi Kobayashi, Hiroyuki Honda

Journal of Bioscience and Bioengineering, 99(1) 72-74, 2005
Convenient and sensitive evaluation of a superoxide anion-generating reagent methyl viologen by Escherichia coli harboring a soxS':: gfp reporter plasmid

A Shibuya, N Tsukagoshi, Ohtsu, I, N Dokyu, R Aono

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68(12) 2637-2639, Dec, 2004
Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli

Ohtsu, I, N Kakuda, N Tsukagoshi, N Dokyu, H Takagi, M Wachi, R Aono

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68(2) 458-461, Feb, 2004
Indigo production by Escherichia coli carrying the phenol hydroxylase gene from Acinetobacter sp strain ST-550 in a water-organic solvent two-phase system

N Doukyu, K Toyoda, R Aono

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 60(6) 720-725, Feb, 2003
Isolation of paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents

Noriyuki Doukyu, Hirokazu Kuwahara, Rikizo Aono

Bioscience, Biotechnology and Biochemistry, 67(2) 334-340, Jan 1, 2003
Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin

R Aunpad, SP Muench, PJ Baker, S Sedelnikova, W Panbangred, N Doukyu, R Aono, DW Rice

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 58 2182-2183, Dec, 2002
Isolation of an Acinetobacter sp ST-550 which produces a high level of indigo in a water-organic solvent two-phase system containing high levels of indole

N Doukyu, T Nakano, Y Okuyama, R Aono

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 58(4) 543-546, Mar, 2002
Cloning, sequence analysis and expression of a gene encoding an organic solvent- and detergent-tolerant cholesterol oxidase of Burkholderia cepacia strain ST-200

N Doukyu, R Aono

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 57(1-2) 146-152, Oct, 2001
Two moles of O-2 consumption and one mole of H2O2 formation during cholesterol peroxidation with cholesterol oxidase from Pseudomonas sp strain ST-200

N Doukyu, R Aono

BIOCHEMICAL JOURNAL, 341(3) 621-627, Aug, 1999
Lithocholic acid side-chain cleavage to produce 17-keto or 22-aldehyde steroids by Pseudomonas putida strain ST-491 grown in the presence of an organic solvent, diphenyl ether

Y Suzuki, N Doukyu, R Aono

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62(11) 2182-2188, Nov, 1998
Effects of organic solvents on indigo formation by Pseudomonas sp. strain ST-200 grown with high levels of indole

N Doukyu, T Arai, R Aono

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62(6) 1075-1080, Jun, 1998
Purification of extracellular cholesterol oxidase with high activity in the presence of organic solvents from Pseudomonas sp. strain ST-200

N Doukyu, R Aono

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 64(5) 1929-1932, May, 1998
Biodegradation of indole at high concentration by persolvent fermentation with Pseudomonas sp. ST-200

N Doukyu, R Aono

EXTREMOPHILES, 1(2) 100-105, May, 1997
Control with organic solvents of efficiency of persolvent cholesterol fermentation by Pseudomonas sp strain ST-200

N Doukyu, H Kobayashi, H Nakajima, R Aono

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 60(10) 1612-1616, Oct, 1996
Stereospecific oxidation of 3 beta-hydroxysteroids by persolvent fermentation with Pseudomonas sp ST-200

R Aono, N Doukyu

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 60(7) 1146-1151, Jul, 1996
OXIDATIVE BIOCONVERSION OF CHOLESTEROL BY PSEUDOMONAS SP STRAIN-ST-200 IN A WATER-ORGANIC SOLVENT 2-PHASE SYSTEM

R AONO, N DOUKYU, H KOBAYASHI, H NAKAJIMA, K HORIKOSHI

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 60(7) 2518-2523, Jul, 1994

Books and Other Publications

極限環境微生物の先端科学と社会実装最前線

監修者, 伊藤政博, 鳴海一成, 道久則之

株式会社エヌ・ティー・エス, Dec, 2023 (ISBN: 9784860438487)
新版ビギナーのための微生物実験ラボガイド

中村聡/中島春紫/伊藤政博/道久則之/八波利恵

May, 2019
極限環境生命―生命の起源を考え、その多様性に学ぶ

道久則之ほか (Role: Joint author, 9章有機溶媒耐性微生物)

コロナ社, Oct, 2014
極限環境生物の産業展開

道久則之, 今中忠行

シーエムシー出版, Aug, 2012
合成生物工学の隆起―有用物質の新たな生産法構築をめざして―

道久則之, 植田充美監修

シーエムシー出版, Mar, 2012
Extremophiles Handbook

道久則之, K. Horikoshi, G. Antranikian, K.O. Ste

Springer, Aug, 2010
微生物利用の大展開

道久則之, 今中忠行, 青野力三

エヌ・ティー・エス, Jul, 2002
特殊環境微生物の検索 : 生物工学実験書

道久則之, 青野力三

培風館, Apr, 2002

Presentations

キノン合成中間体による大腸菌の有機溶媒耐性化機構

道久則之

極限環境生物学会, Dec 8, 2018
高度な有機溶媒耐性を有する大腸菌変異株の解析

道久則之

極限環境生物学会, Dec 8, 2018
2

DOUKYU Noriyuki

DN000063, Nov, 2014
1

DOUKYU Noriyuki

DN000063, Nov, 2014
3

DOUKYU Noriyuki

DN000063, Oct, 2013
Pseudomonas aeruginosa 由来コレステロールオキシダーゼの解析

道久則之, 道久則之, 二瓶翔

極限環境微生物学会, Dec 1, 2012
「大腸菌の高度有機溶媒耐性化の解明

道久則之, 渡邉玲, 道久則之

極限環境微生物学会, Dec 1, 2012
大腸菌におけるacrR およびmarR 遺伝子変異による有機溶媒耐性の向上

道久則之, 渡邉玲, 道久則之

日本農芸化学会関東支部大会, Oct 27, 2012
Improvement in organic solvent-tolerance by double mutations of acrR and marR genes in Escherichia coli

道久則之, R. Watanabe, N. Doukyu

9th International congress on Extremophiles, Sep 10, 2012
Cholesterol oxidases from organic solvent-tolerant bacteria.

道久則之

The 8th International Congress on Extremophiles., Oct, 2010
Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニングと発現

道久則之

日本農芸化学会, Mar 29, 2010
有機溶媒耐性大腸菌の溶媒耐性機構と応用

道久則之

有機溶媒耐性微生物利用技術研究部会シンポジウム(生物工学会), Mar 26, 2010
Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニング

道久則之

極限環境微生物学会, Oct, 2009
Chromobacterium属細菌由来のコレステロールオキシダーゼの精製と解析

道久則之

日本農芸化学会, Mar, 2009
Development of a detergent-tolerant cholesterol oxidase for organic reactions in aqueous medium.

道久則之

The 4th International Symposium on Material Cycling Engineering., Mar, 2009
Crystal Structure of an Extremely Thermostable Cholesterol Oxidase from a Mesophillic Organism.

道久則之, A. Ohtaki, K. Newton, N. Doukyu, M. Sagermann

Protein Society 22nd Annual Symposium., Aug, 2008
Burkholderia cepacia ST-200株由来のコレステロールエステラーゼの解析

道久則之

極限環境微生物学会, Nov, 2007
Burkholderia cepacia ST-200株由来のコレステロールエステラーゼの精製と解析

道久則之

日本農芸化学会2007年度大会, Mar, 2007
Brachybacterium sp. LB25株の生産するG3アミラーゼの精製と諸性質の解析

道久則之, 山岸亘

日本農芸化学会, Mar, 2006
鹿児島湾若尊プロトカルデラ底泥サンプルからの有用微生物のスクリーニング

道久則之, 伊藤政博, 宮崎明日香, 境貴子, 熊坂尚亮

しんかいシンポジウム, Feb, 2006

Professional Memberships

日本農芸化学会

Research Projects

ユビキノン生合成系に着目した高度有機溶媒耐性大腸菌の創製

基盤研究(C), 科学研究費補助金, Apr, 2017 - Mar, 2020

道久則之
高度な安定性を有するコレステロール酸化酵素の触媒機構の解明と高活性化

科学研究費基盤研究（Ｃ）（一般）, 2012 - 2014

道久則之
グラム陰性細菌由来新規コレステロール酸化酵素の高度安定化機構の解明

科学研究費若手研究（Ｂ）, 2009 - 2011

道久則之
オリゴ糖を生成する有機溶媒耐性アミラーゼの精製と諸性質の解析

井上円了記念研究助成金（研究の助成）, 2006 - 2006

道久則之
有機溶剤耐性大腸菌を用いた有機溶剤耐性生体触媒の開発

科学研究費若手研究（Ｂ）, 2005 - 2006

道久則之
有機溶媒耐性プロテアーゼおよびアミラーゼの精製と諸性質の解析

井上円了記念研究助成金（研究の助成）, 2005 - 2005

道久則之
有機溶媒耐性酵素の探索と解析

井上円了記念研究助成金（研究の助成）, 2004 - 2004

道久則之
Improvement of organic solvent tolerance level by directed evolution of AcrAB-TolC efflux pump in Escherichia coli

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2000 - 2002

DOUKYU Noriyuki, TSUKAGOSHI Norihiko
有機溶媒耐性酵素の探索
有機溶媒存在下におけるインジゴの微生物生産
有機溶媒存在下におけるコレステロールの微生物変換
Organic solvent－resistant enzyme
Indigo formation in the presence of organic solvent by a microorganism
Cholesterol conversion in the presence of organic solvent by a microorganism

Industrial Property Rights

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全学自己点検評価委員

2008 - 2008
(No title)

2005 - 2005

極限環境微生物学会若手シンポジウム委員極限環境微生物学会シンポジウムの企画

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