2011年7月
Rapid immunochromatographic test for serum granulysin is useful for the prediction of Stevens-Johnson syndrome and toxic epidermal necrolysis
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
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- 巻
- 65
- 号
- 1
- 開始ページ
- 65
- 終了ページ
- 68
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.jaad.2010.04.042
- 出版者・発行元
- MOSBY-ELSEVIER
Background: Life-threatening adverse drug reactions such as Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) sometimes start with clinical features of ordinary drug-induced skin reactions (ODSRs) and it may be difficult to make a correct diagnosis before severe mucocutaneous erosions occur. We have reported that serum granulysin levels are elevated (cut off: 10 ng/mL) in patients with SJS/TEN before generalized blisters form.
Objective: We sought to develop a rapid detection system for elevated serum granulysin to predict the progression from ODSRs.
Methods: Serum samples from 5 patients with SJS/TEN at 2 to 4 days before mucocutaneous erosions formed were analyzed. Sera from 24 patients with ODSRs and 31 healthy volunteers were also investigated as control subjects. We developed a rapid immunochromatographic assay for the detection of high levels of serum granulysin using two different antigranulysin monoclonal antibodies.
Results: The immunochromatographic test showed positive results for 4 of 5 patients with SJS/TEN but only one patient of 24 with ODSRs. The results correlated closely with those of enzyme-linked immunosorbent assays.
Limitations: The validation of the long-time stability in this test strip has not been investigated.
Conclusion: This novel test enables the prediction of SJS/TEN occurrence in patients even when only features of ODSRs are noted clinically. (J Am Acad Dermatol 2011;65:65-8.)
Objective: We sought to develop a rapid detection system for elevated serum granulysin to predict the progression from ODSRs.
Methods: Serum samples from 5 patients with SJS/TEN at 2 to 4 days before mucocutaneous erosions formed were analyzed. Sera from 24 patients with ODSRs and 31 healthy volunteers were also investigated as control subjects. We developed a rapid immunochromatographic assay for the detection of high levels of serum granulysin using two different antigranulysin monoclonal antibodies.
Results: The immunochromatographic test showed positive results for 4 of 5 patients with SJS/TEN but only one patient of 24 with ODSRs. The results correlated closely with those of enzyme-linked immunosorbent assays.
Limitations: The validation of the long-time stability in this test strip has not been investigated.
Conclusion: This novel test enables the prediction of SJS/TEN occurrence in patients even when only features of ODSRs are noted clinically. (J Am Acad Dermatol 2011;65:65-8.)
- リンク情報
- ID情報
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- DOI : 10.1016/j.jaad.2010.04.042
- ISSN : 0190-9622
- PubMed ID : 21507517
- Web of Science ID : WOS:000292222200008