Masahiro Hosaka

Last updated: Sep 12, 2019 at 14:23

Name	Masahiro Hosaka
Affiliation	Akita Prefectural University
Section	Institute for Molecular and Cellular RegulationBiosignal Genome Resource Center
Degree	Ph.D.(University of Tsukuba), M.Sc.(University of Tsukuba), B.S.(University of Tsukuba)
Research funding number	80311603
ORCID ID	0000-0002-1422-1774

Research Interests

Hypoxia(71) , Imaging(98) , Secretory Granule(6) , peptide hormone(7) , Endocrine(17)

Research Areas

Biomolecular science / Chemical biology /
Biological Science / Cell biology /

Academic & Professional Experience

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Apr 1996

Mar 1998

Researcher, HFSP, University of Texas

1997

1998

: Research fellow, Human Frontier Science　

Oct 1995

Mar 1996

Researcher, HHMI, University of Texas Southwestern medical Institute at Dallas

1995

1996

: Research fellow, Howard Hughes medical　

1995

-: Research fellow, Foundation for Advancement of 　

Program　

Institute　

International Science　

Education

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1995

Graduate School, Division of Bioresearch, University of Tsukuba

1986

1990

Tsukuba Univ.

1990

Faculty of Agriculture, University of Tsukuba

Published Papers

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Culture in 10% O2 enhances the production of active hormones in neuro-endocrine cells by up-regulating the expression of processing enzyme.

Sato E, Maeda Y, Sato Y, Hinata A, Gomi H, Koga D, Torii S, Watanabe T, Hosaka M.

Biochemical Journal 476(5) 795-808 2019 [Refereed]

Impaired Processing of Prohormones in Secretogranin III-Null Mice Causes Maladaptation to an Inadequate Diet and Stress.

Maeda Y, Kudo S, Tsushima K, Sato E, Kubota C, Kayamori A, Bochimoto H, Koga D, Torii S, Gomi H, Watanabe T, Hosaka M

Endocrinology 159(2) 1213-1227 Feb 2018 [Refereed]

The pseudophosphatase phogrin enables glucose-stimulated insulin signaling in pancreatic β-cells.

Torii S, Kubota C, Saito N, Kawano A, Hou N, Kobayashi M, Torii R, Hosaka M, Kitamura T, Takeuchi T, Gomi H

The Journal of biological chemistry 293 5920-5933 Feb 2018 [Refereed]

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

Gomi H, Osawa H, Uno R, Yasui T, Hosaka M, Torii S, Tsukise A

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 65(11) 637-653 Nov 2017 [Refereed]

Oxygen imaging of living cells and tissues using luminescent molecular probes

Yoshihara Toshitada, Hirakawa Yosuke, Hosaka Masahiro, Nangaku Masaomi, Tobita Seiji

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS 30 71-95 Mar 2017 [Refereed]

TORC1 activity is partially reduced under nitrogen starvation conditions in sake yeast Kyokai no. 7, Saccharomyces cerevisiae.

Nakazawa N, Sato A, Hosaka M

Journal of bioscience and bioengineering 121(3) 247-252 Mar 2016 [Refereed]

Expression of Secretogranin III in Chicken Endocrine Cells: Its Relevance to the Secretory Granule Properties of Peptide Prohormone Processing and Bioactive Amine Content.

Gomi H, Morikawa S, Shinmura N, Moki H, Yasui T, Tsukise A, Torii S, Watanabe T, Maeda Y, Hosaka M

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 63(5) 350-366 May 2015 [Refereed]

Partial inhibition of differentiation associated with elevated protein levels of pluripotency factors in mouse embryonic stem cells expressing exogenous EGAM1N homeoprotein.

Sato S, Nakazawa M, Kihara Y, Kubo Y, Sato Y, Kikuchi T, Nonaka A, Sasaki A, Iwashita J, Murata J, Hosaka M, Kobayashi M

Journal of bioscience and bioengineering Mar 2015 [Refereed]

Silylation improves the photodynamic activity of tetraphenylporphyrin derivatives in vitro and in vivo.

Horiuchi H, Hosaka M, Mashio H, Terata M, Ishida S, Kyushin S, Okutsu T, Takeuchi T, Hiratsuka H

Chemistry (Weinheim an der Bergstrasse, Germany) 20(20) 6054-6060 May 2014 [Refereed]

Intracellular and in vivo oxygen sensing using phosphorescent Ir(III) complexes with a modified acetylacetonato ligand.

Yoshihara T, Hosaka M, Terata M, Ichikawa K, Murayama S, Tanaka A, Mori M, Itabashi H, Takeuchi T, Tobita S

Analytical chemistry 87(5) 2710-2717 Mar 2015 [Refereed]

Functional implications of the Golgi and microtubular network in gonadotropes.

Watanabe T, Bochimoto H, Koga D, Hosaka M, Ushiki T

Molecular and cellular endocrinology 385(1-2) 88-96 Mar 2014 [Refereed]

Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells.

Sato S, Morita S, Iha M, Mori Y, Sugawara S, Kasuga K, Kojima I, Ozaki N, Muraguchi H, Okano K, Iwashita J, Murata J, Hosaka M, Kobayashi M

Journal of bioscience and bioengineering 116(2) 141-146 Aug 2013 [Refereed]

Multiple sorting systems for secretory granules ensure the regulated secretion of peptide hormones.

Sun M, Watanabe T, Bochimoto H, Sakai Y, Torii S, Takeuchi T, Hosaka M

Traffic (Copenhagen, Denmark) 14(2) 205-218 Feb 2013 [Refereed]

Chronic exercise enhances insulin secretion ability of pancreatic islets without change in insulin content in non-diabetic rats.

Tsuchiya M, Manabe Y, Yamada K, Furuichi Y, Hosaka M, Fujii NL

Biochemical and biophysical research communications 430(2) 676-682 Jan 2013 [Refereed]

A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network.

Watanabe T, Sakai Y, Koga D, Bochimoto H, Hira Y, Hosaka M, Ushiki T

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 60(8) 588-602 Aug 2012 [Refereed]

Ratiometric molecular sensor for monitoring oxygen levels in living cells.

Yoshihara T, Yamaguchi Y, Hosaka M, Takeuchi T, Tobita S

Angewandte Chemie (International ed. in English) 51(17) 4148-4151 Apr 2012 [Refereed]

Cyclophilin C-associated protein regulation of phagocytic functions via NFAT activation in macrophages.

Yamaguchi R, Hosaka M, Torii S, Hou N, Saito N, Yoshimoto Y, Imai H, Takeuchi T

Brain research 1397 55-65 Jun 2011 [Refereed]

Luminal interaction of phogrin with carboxypeptidase E for effective targeting to secretory granules.

Saito N, Takeuchi T, Kawano A, Hosaka M, Hou N, Torii S

Traffic (Copenhagen, Denmark) 12(4) 499-506 Apr 2011 [Refereed]

Synthesis and Properties of Fluorescent Biological Molecules Labeled with Novel Silylated Perylene Derivative

Sato Yuzuru, Ozaki Akiko, Hosaka Masahiro, Moriguchi Tomohisa, Shinozuka Kazuo

SILICON SCIENCE AND ADVANCED MICRO-DEVICE ENGINEERING I 459 63-+ 2011 [Refereed]

Cholesterol biosynthesis pathway intermediates and inhibitors regulate glucose-stimulated insulin secretion and secretory granule formation in pancreatic beta-cells.

Tsuchiya M, Hosaka M, Moriguchi T, Zhang S, Suda M, Yokota-Hashimoto H, Shinozuka K, Takeuchi T

Endocrinology 151(10) 4705-4716 Oct 2010 [Refereed]

Phosphorescent light-emitting iridium complexes serve as a hypoxia-sensing probe for tumor imaging in living animals.

Zhang S, Hosaka M, Yoshihara T, Negishi K, Iida Y, Tobita S, Takeuchi T

Cancer research 70(11) 4490-4498 Jun 2010 [Refereed]

Secretogranin III: a bridge between core hormone aggregates and the secretory granule membrane.

Hosaka M, Watanabe T

Endocrine journal 57(4) 275-286 2010 [Refereed]

Secretogranin II binds to secretogranin III and forms secretory granules with orexin, neuropeptide Y, and POMC.

Hotta K, Hosaka M, Tanabe A, Takeuchi T

The Journal of endocrinology 202(1) 111-121 Jul 2009 [Refereed]

A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.

Han L, Suda M, Tsuzuki K, Wang R, Ohe Y, Hirai H, Watanabe T, Takeuchi T, Hosaka M

Molecular endocrinology (Baltimore, Md.) 22(8) 1935-1949 Aug 2008 [Refereed]

Reactive oxygen species-mediated pancreatic beta-cell death is regulated by interactions between stress-activated protein kinases, p38 and c-Jun N-terminal kinase, and mitogen-activated protein kinase phosphatases.

Hou N, Torii S, Saito N, Hosaka M, Takeuchi T

Endocrinology 149(4) 1654-1665 Apr 2008 [Refereed]

Sorting mechanism of peptide hormones and biogenesis mechanism of secretory granules by secretogranin III, a cholesterol-binding protein, in endocrine cells.

Takeuchi T, Hosaka M

Current diabetes reviews 4(1) 31-38 Feb 2008 [Refereed]

Effects of a depot formulation of the GnRH agonist leuprorelin on the ultrastructure of male rat pituitary gonadotropes.

Kitahara K, Sakai Y, Hosaka M, Hira Y, Kakizaki H, Watanabe T

Archives of histology and cytology 70(2) 79-93 Jul 2007 [Refereed]

A subset of p23 localized on secretory granules in pancreatic beta-cells.

Hosaka M, Watanabe T, Yamauchi Y, Sakai Y, Suda M, Mizutani S, Takeuchi T, Isobe T, Izumi T

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55(3) 235-245 Mar 2007 [Refereed]

The sorting mechanism to secretory granules: Emerging concepts with secretogranin III

Hosaka Masahiro

SEIKAGAKU 79(2) 166-170 Feb 2007 [Refereed]

[Sorting mechanism of secretory granules: Emerging concepts with secretogranin III].

Hosaka M

Seikagaku. The Journal of Japanese Biochemical Society 79(2) 166-170 Feb 2007 [Refereed]

Molecular probes for sensing the cholesterol composition of subcellular organelle membranes.

Wang R, Hosaka M, Han L, Yokota-Hashimoto H, Suda M, Mitsushima D, Torii S, Takeuchi T

Biochimica et biophysica acta 1761(10) 1169-1181 Oct 2006 [Refereed]

Addition of phosphotungstic acid to ethanol for dehydration improves both the ultrastructure and antigenicity of pituitary tissue embedded in LR White acrylic resin.

Sakai Y, Hosaka M, Hira Y, Watanabe T

Archives of histology and cytology 68(5) 337-347 Dec 2005 [Refereed]

Interaction between secretogranin III and carboxypeptidase E facilitates prohormone sorting within secretory granules.

Hosaka M, Watanabe T, Sakai Y, Kato T, Takeuchi T

Journal of cell science 118(Pt 20) 4785-4795 Oct 2005 [Refereed]

Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene.

Aihara M, Sugawara K, Torii S, Hosaka M, Kurihara H, Saito N, Takeuchi T

Laboratory investigation; a journal of technical methods and pathology 84(12) 1581-1592 Dec 2004 [Refereed]

Immunocytochemical localization of secretogranin III in the endocrine pancreas of male rats.

Sakai Y, Hosaka M, Yoshinaga A, Hira Y, Harumi T, Watanabe T

Archives of histology and cytology 67(1) 57-64 Mar 2004 [Refereed]

Functional localization of proprotein-convertase furin and its substrate TGFbeta in EGF receptor-expressing gastric chief cells.

Fujisawa T, Kamimura H, Hosaka M, Torii S, Izumi T, Kuwano H, Takeuchi T

Growth factors (Chur, Switzerland) 22(1) 51-59 Mar 2004 [Refereed]

Secretogranin III binds to cholesterol in the secretory granule membrane as an adapter for chromogranin A.

Hosaka M, Suda M, Sakai Y, Izumi T, Watanabe T, Takeuchi T

The Journal of biological chemistry 279(5) 3627-3634 Jan 2004 [Refereed]

Parathyroid hormone-related protein induces insulin expression through activation of MAP kinase-specific phosphatase-1 that dephosphorylates c-Jun NH2-terminal kinase in pancreatic beta-cells.

Zhang B, Hosaka M, Sawada Y, Torii S, Mizutani S, Ogata M, Izumi T, Takeuchi T

Diabetes 52(11) 2720-2730 Nov 2003 [Refereed]

Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands.

Sakai Y, Hosaka M, Hira Y, Harumi T, Ohsawa Y, Wang H, Takeuchi T, Uchiyama Y, Watanabe T

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51(2) 227-238 Feb 2003 [Refereed]

Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic beta-cells.

Hosaka M, Watanabe T, Sakai Y, Uchiyama Y, Takeuchi T

Molecular biology of the cell 13(10) 3388-3399 Oct 2002 [Refereed]

The rab27a/granuphilin complex regulates the exocytosis of insulin-containing dense-core granules.

Yi Z, Yokota H, Torii S, Aoki T, Hosaka M, Zhao S, Takata K, Takeuchi T, Izumi T

Mol Cell Biol. 22(6) 1858-1867 Mar 2002 [Refereed]

Identification of a Chromogranin A Domain that Mediates Binding to Secretogranin III and Cells and Pancreatic β-Cells

Mol Biol Cell 13, 3388-3399 2002

Cyclosporine A-induced hypertension involves synapsin in renal sensory nerve endings.

Zhang W, Li JL, Hosaka M, Janz R, Shelton JM, Albright GM, Richardson JA, Südhof TC, Victor RG

Proc. Notl. Acad. Sci. 97(17) 9765-9770 Aug 2000 [Refereed]

A phospho-switch controls the dynamic association of synapsins with synaptic vesicles

Hosaka M, Hammer RE, Südhof TC

NEURON 24(2) 377-387 Oct 1999 [Refereed]

A conformational switch in syntaxin during exocytosis:role of munc18.

Dulubova I, Sugita S, Hill S, Hosaka M, Fernandez I, Südhof TC, Rizo J

EMBO J 18(16) 4372-4382 Aug 1999 [Refereed]

Homo- and heterodimerization of synapsins.

Hosaka M, Südhof TC

J.Biol. Chem 274(24) 16747-16753 Jun 1999 [Refereed]

Synapsin III, a novel synapsin with an unusual regulation by Ca2+.

Hosaka M, Südhof TC

The Journal of biological chemistry 273(22) 13371-13374 May 1998 [Refereed]

Synapsin I is structurally similar to ATP-utilizing enzymes.

Esser L, Wang CR, Hosaka M, Smagula CS, Südhof TC, Deisenhofer J

The EMBO journal 17(4) 977-984 Feb 1998 [Refereed]

Synapsins I and II are ATP-binding proteins with differential Ca2+ regulation.

Hosaka M, Südhof TC

The Journal of biological chemistry 273(3) 1425-1429 Jan 1998 [Refereed]

Synapsin III,A novel synapsin with an usual regulation by Ca²⁺

J. Biol. Chem 27322,13371-13374 1998

Synapsin I is structually Similar to ATP-utilizing enzymes

EMBO J. 17,977-984 1998

Synapsin I and II are ATP-binding proteins with differential Ca2+ regulation

J. Biol. Chem 273,1425-1429 1998

Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6(ARF1-ARF6).

Hosaka M, Toda K, Takatsu H, Torii S, Murakami K, Nakayama K

J Biochem (Tokyo) 120(4) 813-819 Oct 1996 [Refereed]

Differential effects of Brefeldin A on intracellular localization of ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6) cloned from mouse."Jointly-worked"

J.Biochem.(Tokyo) 1996

PACE4A is a ubiquitous endoprotease that has similar but not identical substrate specificity to other Kex2-like processing endoproteases.

Hosaka, M., Murakami, K., Nakayama,K

Biomed.Res. 15 383-390 1994 [Refereed]

Cloning and Sequence Analysis of mouse cDNAs Encoding Preprotackykinin A and B.

Kako K, Munekata E, Hosaka M, Murakami K, Nakayama K

Biomed.Res. 14 253-259 1993 [Refereed]

Consensus sequence for precursor processing at mono-arginyl sites. Evidence for the involvement of a Kex2-like endoprotease in precursor cleavages at both dibasic and mono-arginyl sites.

Nakayama K, Watanabe T, Nakagawa T, Kim WS, Nagahama M, Hosaka M, Hatsuzawa K, Kondoh-Hashiba K, Murakami K

The Journal of biological chemistry 267(23) 16335-16340 Aug 1992 [Refereed]

Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease. Its testis-specific expression.

Nakayama K, Kim WS, Torii S, Hosaka M, Nakagawa T, Ikemizu J, Baba T, Murakami K

The Journal of biological chemistry 267(9) 5897-5900 Mar 1992 [Refereed]

Arg-X-Lys/Arg-Argmotif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway

Hosaka M, Nagahama M, Kim WS, Watanabe T, Hatsuzawa K, Ikemizu J, Murakami K, Nakayama K

J. Biol. Chem 266,12127-12130(19) 12127-12130 Jul 1991 [Refereed]

Cloning and functional expression of a novel endoprotease involved in prohormone processing at dibasic sites.

Nakayama K, Hosaka M, Hatsuzawa K, Murakami K

Journal of biochemistry 109(6) 803-806 Jun 1991 [Refereed]

Structure and expression of mouse furin, a yeast Kex2-related protease. Lack of processing of coexpressed prorenin in GH4C1 cells.

Hatsuzawa K, Hosaka M, Nakagawa T, Nagase M, Shoda A, Murakami K, Nakayama K

The Journal of biological chemistry 265(36) 22075-22078 Dec 1990 [Refereed]

Conference Activities & Talks

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Sorting Mechanisms of Prohormones from the Trans-Golgi Network to Secretory Granules: Concepts with Granin-Family Protein [Invited]

Masahiro Hosaka

27 Jun 2019

Golgi Apparatus Structures and Function: Sorting Mechanisms of Prohormones from the Trans-Golgi Network to Secretory Granules: Concepts with Granin-Family Protein. [Invited]

Masahiro Hosaka

Gordon Research Conferences; (New London, U.S.A.) 23 Jul 2014

Sorting mechanism of regulated secretory proteins by Secretogranin III [Invited]

Masahiro Hosaka

15th International Symposia on Chromaffin Cell Biology; (Merida Mexico) 2009

Iridium complex: a phosphorescent light-emitting diode material, serves as a novel tool for imaging hypoxic tumor tissues. [Invited]

Masahiro Hosaka

International Symposium for Environmental Medicine; (Nagoya, Japan) 2008

Secretogranin III: a connecting bridge between secretory proteins and the cholesterol-rich granule membrane. [Invited]

Masahiro Hosaka

International Conference for Granulogenesis; (Bethesda, U.S.A.) 2006

The sorting mechanism to Secretory Granules: Emerging Concepts with Secretogranin III. [Invited]

Masahiro Hosaka

Gordon Research Conferences; (New London, U.S.A.) 2006

Secretogranin III: a connecting bridge between secretory proteins and the cholesterol-rich granule membrane. [Invited]

Masahiro Hosaka

16th International Congress of the International Federation of Associations of Anatomists; (Kyoto, Japan) 2004

Sorting Mechanisms of Prohormones from the Trans-Golgi Network to Secretory Granules: Concepts with Granin-Family Protein [Invited]

Masahiro Hosaka

22 Sep 2018

Association Memberships

THE MOLECULAR BIOLOGY SOCIETY OF JAPAN(2615) , Endocrine Society(43) , Japanese Society FOR Molecular Imaging(28) , JAPAN SOCIETY FOR CELL BIOLOGY(354) , THE JAPANESE BIOCHEMICAL SOCIETY(1744)