論文

査読有り 最終著者 責任著者
2018年4月1日

CD146 positive human dental pulp stem cells promote regeneration of dentin/pulp-like structures.

Human cell
  • Mikiko Matsui
  • ,
  • Tomoko Kobayashi
  • ,
  • Takeo W Tsutsui

31
2
開始ページ
127
終了ページ
138
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s13577-017-0198-2
出版者・発行元
Springer Tokyo

CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146+) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146−) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146+ and CD146− cells, as well as mixtures composed of 25% CD146+ cells and 75% CD146− cells (CD146+/−). Cell growth assays indicated that CD146+ cells exhibit an approximate 3–4 h difference in doubling time, compared with CD146− cells. Cell cycle distributions were determined by flow cytometry analysis. The low percentage of CD146+ cells’ DNA content in G0/G1 phase were compared with CD146− and non-separated cells. In contrast to CD146− and non-separated cells, prompt mineralization was observed in CD146+ cells. Subsequently, qRT-PCR revealed high mRNA expression of CD146 and Alkaline phosphatase in mineralization-induced CD146+ cells. CD146+ cells were also observed high adipogenic ability by Oil red O staining. Histological examinations revealed an increased area of dentin/pulp-like structures in transplanted CD146+ cells, compared with CD146− and CD146+/− cells. Immunohistochemical studies detected dentin matrix protein-1 (DMP1) and dentin sialophosphoprotein (DSPP), as well as human mitochondria, in transplanted DPSCs. Co-expression of CD146 and GFP indicated that CD146 was expressed in transplanted CD146+ cells. CD146+ cells may promote mineralization and generate dentin/pulp-like structures, suggesting a role in self-renewal of stem cells and dental pulp regenerative therapy.

リンク情報
DOI
https://doi.org/10.1007/s13577-017-0198-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29313241
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852189
ID情報
  • DOI : 10.1007/s13577-017-0198-2
  • ISSN : 1749-0774
  • ISSN : 0914-7470
  • PubMed ID : 29313241
  • PubMed Central 記事ID : PMC5852189
  • SCOPUS ID : 85044109572

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