論文

2004年4月

Innervation of rat iris by trigentinal and ciliary neurons expressing pChAT, a novel splice variant of choline acetyltransferase

JOURNAL OF COMPARATIVE NEUROLOGY
  • O Yasuhara
  • ,
  • Y Aimi
  • ,
  • A Shibano
  • ,
  • A Matsuo
  • ,
  • JP Bellier
  • ,
  • M Park
  • ,
  • Tooyama, I
  • ,
  • H Kimura

472
2
開始ページ
232
終了ページ
245
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cne.20077
出版者・発行元
WILEY-LISS

We have recently discovered a splice variant of choline acetyltransferase (ChAT) mRNA and designated the variant protein pChAT because of its preferential expression in peripheral neuronal structures. In this study, the presence of pChAT in rat iris was examined by immunohistochemistry and Western blot using a pChAT antiserum, in combination with RT-PCR analysis and ChAT enzyme assay. For comparison, the conventional ChAT (cChAT) was studied in parallel. By pChAT immunohistochemistry, intense labeling was found to occur in nerve fibers of the iris and in neurons of the ciliary and trigeminal ganglia. Denervation studies, analyzed by semiquantitative morphometry, indicated that these iridial pChAT fibers originated about half from the ciliary ganglion and the other half from the trigeminal ganglion. The presence of pChAT protein in the iris and trigeminal ganglion was confirmed by Western blot. The expression of pChAT mRNA in the ciliary and trigeminal ganglia was proved by RT-PCR. Although cChAT protein and mRNA were detected in the ciliary ganglion, neither was detectable in the trigeminal ganglion. The contributions of the ciliary and trigeminal ganglia to the iridial ChAT enzyme activity were verified by the present ChAT assay. Here, we provide evidence that iridial pChAT nerves are composed of postganglionic parasympathetic efferents from the ciliary ganglion and, more interestingly, somatic sensory afferents of the trigeminal ophthalmic nerve. (C) 2004 Wiley-Liss, Inc.

リンク情報
DOI
https://doi.org/10.1002/cne.20077
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220626700010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/cne.20077
  • ISSN : 0021-9967
  • Web of Science ID : WOS:000220626700010

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