論文

査読有り
2011年1月

Transporter Associated with Antigen Processing-Like (ABCB9) Stably Expressed in Chinese Hamster Ovary-K1 Cells Is Sorted to the Microdomains of Lysosomal Membranes

BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • Yasuyuki Fujimoto
  • ,
  • Aya Kamakura
  • ,
  • Yu Motohashi
  • ,
  • Ayako Ohashi-Kobayashi
  • ,
  • Masatomo Maeda

34
1
開始ページ
36
終了ページ
40
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1248/bpb.34.36
出版者・発行元
PHARMACEUTICAL SOC JAPAN

The carboxyl terminus of a human ATP-binding cassette (ABC) transporter, transporter associated with antigen processing (TAP)-like (TAPL), was tagged with green fluorescence protein (GFP), and the resulting fusion protein (TAPL-GFP) was stably expressed in Chinese hamster ovary (CHO)-K1 cells. The GFP signal was co-localized with that of LysoTracker but not that of MitoTracker, as visualized under a microscope. TAPL-GFP was co-sedimented with lysosomal marker cathepsin D on Percoll density gradient centrifugation. These results indicated that TAPL is a lysosomal ABC transporter but not a mitochondrial one. It was not solubilized completely with a non-ionic detergent under ice-cold conditions, and was co-sedimented with flotillin-1 on sucrose density gradient centrifugation. A similar result was obtained with high pH-treatment. Furthermore, treatment with methyl-beta-cyclodextrin resulted in an altered distribution of TAPL-GFP. These results suggest that TAPL may be localized to the microdomains (lipid rafts) of lysosomal membranes enriched in cholesterol.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1248/bpb.34.36
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000285699100006&DestApp=WOS_CPL

エクスポート
BibTeX RIS