論文

査読有り
2017年10月

Artificial acceleration of mammalian cell reprogramming by bacterial proteins

GENES TO CELLS
  • Takashi Ikeda
  • ,
  • Ikuo Uchiyama
  • ,
  • Mio Iwasaki
  • ,
  • Tetsuhiko Sasaki
  • ,
  • Masato Nakagawa
  • ,
  • Keisuke Okita
  • ,
  • Shinji Masui

22
10
開始ページ
918
終了ページ
928
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/gtc.12519
出版者・発行元
WILEY

The molecular mechanisms of cell reprogramming and differentiation involve various signaling factors. Small molecule compounds have been identified to artificially influence these factors through interacting cellular proteins. Although such small molecule compounds are useful to enhance reprogramming and differentiation and to show the mechanisms that underlie these events, the screening usually requires a large number of compounds to identify only a very small number of hits (e.g., one hit among several tens of thousands of compounds). Here, we show a proof of concept that xenospecific gene products can affect the efficiency of cell reprogramming to pluripotency. Thirty genes specific for the bacterium Wolbachia pipientis were forcibly expressed individually along with reprogramming factors (Oct4, Sox2, Klf4 and c-Myc) that can generate induced pluripotent stem cells in mammalian cells, and eight were found to affect the reprogramming efficiency either positively or negatively (hit rate 26.7%). Mechanistic analysis suggested one of these proteins interacted with cytoskeleton to promote reprogramming. Our results raise the possibility that xenospecific gene products provide an alternative way to study the regulatory mechanism of cell identity.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1111/gtc.12519
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-85026752117&origin=inward
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000413005200006&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-85026752117&partnerID=MN8TOARS
URL
http://orcid.org/0000-0002-9197-5000