論文

査読有り
2017年4月

Complete genome sequence and expression profile of the commercial lytic enzyme producer Lysobacter enzymogenes M497-1

DNA RESEARCH
  • Hideto Takami
  • ,
  • Atsushi Toyoda
  • ,
  • Ikuo Uchiyama
  • ,
  • Takehiko Itoh
  • ,
  • Yoshihiro Takaki
  • ,
  • Wataru Arai
  • ,
  • Shinro Nishi
  • ,
  • Mikihiko Kawai
  • ,
  • Kazuo Shin-ya
  • ,
  • Haruo Ikeda

24
2
開始ページ
169
終了ページ
177
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/dnares/dsw055
出版者・発行元
OXFORD UNIV PRESS

Lysobacter enzymogenes M497-1 is a producer of commercialized achromopeptidase and is expected to harbour genes encoding various other antimicrobial enzymes. Here, we present the complete sequence of the genome of M497-1 and the expression profiles of the genes for various antimicrobial enzymes. Of the 117 peptidase-encoding genes found in the 6.1-Mb genome of M497-1, 15 genes (aside from the gene encoding the achromopeptidase) were expressed at a level higher than that of the average ribosomal protein genes in the 24-h culture. Thus, the strain was found more valuable than hitherto considered. In addition, M497-1 harbours 98 genes involved in the biosynthesis of various natural products, 16 of which are M497-1-specific across 4Lysobacter species. A gene cluster starting at LEN_2603 through LEN_2673 among the 98 genes closely resembled the lysobactin biosynthesis gene cluster ofLysobacter sp. ATCC 53042. It is likely that M497-1 may produce lysobactin or related antibacterial compounds. Furthermore, comparative genomic analysis of M497-1 and four otherLysobacter species revealed that their core genome structure comprises 3,737 orthologous groups. Our findings are expected to advance further biotechnological application ofLysobacter spp. as a promising source of natural bioactive compounds.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1093/dnares/dsw055
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-85019128472&origin=inward
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000400913000006&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-85019128472&partnerID=MN8TOARS
URL
http://orcid.org/0000-0002-9197-5000