2009年12月
Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
GENES TO CELLS
- 巻
- 14
- 号
- 12
- 開始ページ
- 1395
- 終了ページ
- 1404
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1365-2443.2009.01356.x
- 出版者・発行元
- WILEY-BLACKWELL PUBLISHING, INC
Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be reprogrammed to hiPSCs and miPSCs with efficiencies for AM cells of 0.02% and 0.1%, respectively. Both hiPSC and miPSCs are indistinguishable from embryonic stem cells in colony morphology, expression of pluripotency markers, global gene expression profile, DNA methylation status of OCT4 and NANOG, teratoma formation and, in the case of miPSCs, generation of germline transmissible chimeric mice. As copious amounts of human AM cells can be collected without invasion, and stored long term by conventional means without requirement for in vitro culture, they represent an ideal source for cell banking and subsequent 'on demand' generation of hiPSCs for personal regenerative and pharmaceutical applications.
- リンク情報
-
- DOI
- https://doi.org/10.1111/j.1365-2443.2009.01356.x
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201002243947797878
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19912344
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000272380300004&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1111/j.1365-2443.2009.01356.x
- ISSN : 1356-9597
- J-Global ID : 201002243947797878
- PubMed ID : 19912344
- Web of Science ID : WOS:000272380300004