2002年3月
Crystal structures of 7-methylguanosine 5 '-triphosphate (m(7)GTP)- and P-1-7-methylguanosine-P-3-adenosine-5 ',5 '-triphosphate (m7GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region
BIOCHEMICAL JOURNAL
- 巻
- 362
- 号
- 3
- 開始ページ
- 539
- 終了ページ
- 544
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1042/0264-6021:3620539
- 出版者・発行元
- PORTLAND PRESS
The crystal structures of the full-length human eukaryotic initiation factor (eIF) 4E complexed with two mRNA cap analogues [7-methylguanosine 5'-triphosphate (m(7)GTP) and P-3-7-methylguanosine-P-3-adenosine-5',5'-triphosphate (m(7)GpppA)] were determined at 2.0 Angstrom resolution (where 1 Angstrom = 0.1 nm). The flexibility of the C-terminal loop region of eIF4E complexed with m(7)GTP was significantly reduced when complexed with m(7)GpppA, suggesting the importance of the second nucleotide in the mRNA cap structure for the biological function of eIF4E, especially the fixation and orientation of the C-terminal loop region, including the eIF4E phosphorylation residue. The present results provide the structural basis for the biological function of both N- and C-terminal mobile regions of eIF4E in translation initiation, especially the regulatory function through the switch on/off of eIF4E-binding protein-eIF4E phosphorylation.
- リンク情報
-
- DOI
- https://doi.org/10.1042/0264-6021:3620539
- CiNii Articles
- http://ci.nii.ac.jp/naid/80015202976
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11879179
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000174529500003&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1042/0264-6021:3620539
- ISSN : 0264-6021
- CiNii Articles ID : 80015202976
- PubMed ID : 11879179
- Web of Science ID : WOS:000174529500003