2007年6月
Regulation of DNA nucleases by molecular crowding
NUCLEIC ACIDS RESEARCH
- ,
- ,
- 巻
- 35
- 号
- 12
- 開始ページ
- 4086
- 終了ページ
- 4093
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1093/nar/gkm445
- 出版者・発行元
- OXFORD UNIV PRESS
Here, we examined the effects of molecular crowding on the function, structure and stability of nucleases. We found that the hydrolysis of a 29-mer double-stranded DNA by the endonucleases DNase I and S1 nuclease was substantially enhanced by molecular crowding using polyethylene glycol ( PEG); however, molecular crowding had little effect on hydrolysis by exo III and exo I exonucleases. Moreover, kinetic analysis showed that the maximum velocity for the reaction of DNase I at 25 degrees C was increased from 0.1 to 2.7 mu M/min by molecular crowding with 20% (w/v) PEG, whereas that of exonuclease I at 37 degrees C decreased from 2.2 to 0.4 kM/min. In contrast, molecular crowding did not significantly affect the Michaelis constant of DNase I or exonuclease I. These results indicate that molecular crowding has different effects on the catalytic activities of exonucleases and endonucleases.
- リンク情報
-
- DOI
- https://doi.org/10.1093/nar/gkm445
- CiNii Articles
- http://ci.nii.ac.jp/naid/80018388775
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17567601
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000247817700020&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1093/nar/gkm445
- ISSN : 0305-1048
- CiNii Articles ID : 80018388775
- PubMed ID : 17567601
- Web of Science ID : WOS:000247817700020