1998年11月
Homologous recombination, but not DNA repair, is reduced in vertebrate cells deficient in RAD52
MOLECULAR AND CELLULAR BIOLOGY
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- 巻
- 18
- 号
- 11
- 開始ページ
- 6430
- 終了ページ
- 6435
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- AMER SOC MICROBIOLOGY
Rad52 plays a pivotal role in double-strand break (DSB) repair and genetic recombination in Saccharomyces cerevisiae, where mutation of this gene leads to extreme X-ray sensitivity and defective recombination, Yeast Rad51 and Rad52 interact, as do their human homologues, which stimulates Radii-mediated DNA strand exchange in vitro, suggesting that Rad51 and Rad52 act cooperatively. To define the role of Rad52 in vertebrates, we generated RAD52(-/-) mutants of the chicken B-cell line DT40. Surprisingly, RAD52(-/-) cells were not hypersensitive to DNA damages induced by gamma-irradiation, methyl methanesulfonate, or cis platinum(II)diammine dichloride (cisplatin). Intrachromosomal recombination, measured by immunoglobulin gene conversion, and radiation-induced Rad51 nuclear focus formation, which is a putative intermediate step during recombinational repair, occurred as frequently in RAD52(-/-) cells as in,wild-type cells. Targeted integration frequencies, however, were consistently reduced in RAD52(-/-) cells, showing a clear role for Rad52 in genetic recombination. These findings reveal striking differences between S. cerevisiae and vertebrates in the functions of RAD51 and RAD52.
- リンク情報
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- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902175316942853
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/9774659
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000076512900026&DestApp=WOS_CPL
- ID情報
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- ISSN : 0270-7306
- J-Global ID : 200902175316942853
- PubMed ID : 9774659
- Web of Science ID : WOS:000076512900026