2016年8月
Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
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- 巻
- 52
- 号
- 7
- 開始ページ
- 742
- 終了ページ
- 748
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11626-016-0038-9
- 出版者・発行元
- SPRINGER
In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCENY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.
- リンク情報
- ID情報
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- DOI : 10.1007/s11626-016-0038-9
- ISSN : 1071-2690
- eISSN : 1543-706X
- PubMed ID : 27130679
- Web of Science ID : WOS:000383594100004