Mahito Ohkuma

Profile Information

Affiliation: School of Medicine Faculty of Medicine, Fujita Health University

Degree: Ph.D(*Himeji Institute of Technology*)

J-GLOBAL ID: 200901080823507854
researchmap Member ID: 1000306282

External link: http://www.fujita-hu.ac.jp/~m-ohkuma/index.html

Research Interests

Research Areas

Research History

Education

- 2000

Himeji Institute of Technology
- 2000

Graduate School, Division of Natural Science, Himeji Institute of Technology
- 1995

Himeji Institute of Technology
- 1995

Faculty of Science, Himeji Institute of Technology

Awards

2001

日本動物学会論文賞
2001

Zoological Science Award

Papers

Interareal synaptic inputs underlying whisking-related activity in the primary somatosensory barrel cortex

Masahiro Kawatani, Kayo Horio, Mahito Ohkuma, Wan-Ru Li, Takayuki Yamashita

The Journal of Neuroscience, JN-RM, Dec 1, 2023
Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons.

Noriko Hiramatsu, Naoki Yamamoto, Mahito Ohkuma, Noriaki Nagai, Ei-Ichi Miyachi, Kumiko Yamatsuta, Kazuyoshi Imaizumi

Medical molecular morphology, 55(4) 292-303, Aug 6, 2022
Long-range axonal projections of transplanted mouse embryonic stem cell-derived hypothalamic neurons into adult mouse brain.

Miho Kawata, Yu Kodani, Mahito Ohkuma, Ei-Ichi Miyachi, Yoko S Kaneko, Akira Nakashima, Hidetaka Suga, Toshiki Kameyama, Kanako Saito, Hiroshi Nagasaki

PloS one, 17(11) e0276694, 2022
Effects of Gonadal Hormones On Glutamatergic Circuits in the Retina

Mahito Ohkuma, Takuma Maruyama, Toshiyuki Ishii, Nozomi Igarashi, Keiko Azuma, Tatsuya Inoue, Ryo Obata, Ei-ichi Miyachi, Makoto Kaneda

Oct 25, 2021
Novel Technique for Retinal Nerve Cell Regeneration with Electrophysiological Functions Using Human Iris-Derived iPS Cells.

Naoki Yamamoto, Noriko Hiramatsu, Mahito Ohkuma, Natsuko Hatsusaka, Shun Takeda, Noriaki Nagai, Ei-Ichi Miyachi, Masashi Kondo, Kazuyoshi Imaizumi, Masayuki Horiguchi …

Cells, 10(4), Mar 28, 2021
A subset of cone bipolar cells expresses the Na+ channel SCN2A in the human retina

Fusao Kawai, Mahito Ohkuma, Masayuki Horiguchi, Hiroshi Ichinose, Ei-ichi Miyachi

Experimental Eye Research, 202 108299-108299, Jan, 2021
The Effect of Histamine on Inward and Outward Currents in Mouse Retinal Amacrine Cells

Kayo Horio, Mahito Ohkuma, Ei-ichi Miyachi

Cellular and Molecular Neurobiology, 38(3) 757-767, Apr 1, 2018 Peer-reviewed
Optical measurement of glutamate in slice preparations of the mouse retina

M. Ohkuma, M. Kaneda, S. Yoshida, A. Fukuda, E. Miyachi

Neuroscience Research, 137 23-29, 2018 Peer-reviewed
Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods

Naoki Yamamoto, Yoshinao Kato, Atsushi Sato, Noriko Hiramatsu, Hiromi Yamashita, Mahito Ohkuma, Ei-ichi Miyachi, Masayuki Horiguchi, Koji Hirano, Hajime Kojima

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 52(7) 742-748, Aug, 2016 Peer-reviewed
Acetylcholine enhances excitability by lowering the threshold of spike generation in Olfactory receptor cells

Mahito Ohkuma, Fusao Kawai, Ei-ichi Miyachi

Journal of Neurophysiology, 110(9) 2082-2089, Nov 1, 2013 Peer-reviewed
A type of the human retinal bipolar cell expresses the type II sodium channel

Mahito Ohkuma, Fusao Kawai, Masayuki Horiguchi, Hiroshi Ichinose, Ei-ichi Miyachi

NEUROSCIENCE RESEARCH, 71 E147-E147, 2011 Peer-reviewed
Histamine and histamine receptor of ganglion cells in the gerbil retina

Hideki Imada, Masahiro Kokubo, Mahito Ohkuma, Toshi-aki Kato, Ei-ichi Miyachi

JOURNAL OF PHYSIOLOGICAL SCIENCES, 60 S136-S136, 2010 Peer-reviewed
Histamine modulates membrane currents in retinal ganglion cells of the gerbil

Mahito Ohkuma, Hideki Imada, Ei-ichi Miyachi

NEUROSCIENCE RESEARCH, 68 E151-E151, 2010 Peer-reviewed
ACETYLCHOLINE AFFECTS THE SIGNAL ENCODING IN NEWT OLFACTORY RECEPTOR CELLS

Mahito Ohkuma, Fusao Kawai, Ei-ichi Miyachi

JOURNAL OF PHYSIOLOGICAL SCIENCES, 59 148-148, 2009 Peer-reviewed
THE POSTNATAL EXPRESSION OF HISTAMINE RECEPTORS IN THE GERBIL RETINA

Hideki Imada, Masahiro Kokubo, Mahito Ohkuma, Toshi-aki Kato, Ei-ichi Miyachi

JOURNAL OF PHYSIOLOGICAL SCIENCES, 59 139-139, 2009 Peer-reviewed
Immunohistochemical and calcium-imaging analyses of histamine and histamine receptors in mammalian retinal neurons

Hideki Imada, Mahito Ohkuma, Masahiro Kokubo, Toshi-aki Kato, Ei-ichi Miyachi

NEUROSCIENCE RESEARCH, 65 S14-S14, 2009 Peer-reviewed
Localization of histamine H2 receptor in the gerbil retina Mahito Ohkuma, Hideki Imada, Ei-ichi Miyachi

Mahito Ohkuma, Hideki Imada, Ei-ichi Miyachi

NEUROSCIENCE RESEARCH, 65 S205-S205, 2009 Peer-reviewed
Histamine affects olfactory signals in the olfactory epithelium and bulb

Mahito Ohkuma, Hideya Mimura, Hideki Imada, Kensei Naito, Ei-ichi Miyachi

NEUROSCIENCE RESEARCH, 61 S179-S179, 2008 Peer-reviewed
Isolation of multipotent stem cells from mouse adipose tissue

Naoki Yamamoto, Hirohiko Akamatsu, Seiji Hasegawa, Takaaki Yamada, Satoru Nakata, Mahito Ohkuma, Ei-Ichi Miyachi, Tohru Marunouchi, Kayoko Matsunaga

JOURNAL OF DERMATOLOGICAL SCIENCE, 48(1) 43-52, Oct, 2007 Peer-reviewed
Requirement of the tumour suppressor APC for the clustering of PSD-95 and AMPA receptors in hippocampal neurons

Atsushi Shimomura, Mahito Ohkuma, Akiko Iizuka-Kogo, Kazuyoshi Kohu, Ryuji Nomura, Ei-ichi Miyachi, Tetsu Akiyama, Takao Senda

EUROPEAN JOURNAL OF NEUROSCIENCE, 26(4) 903-912, Aug, 2007 Peer-reviewed

Misc.

松葉エキス摂取後におけるスナネズミの抗酸化作用について

金子千之, 加藤好光, 藤田公和, 日高聰, 安倍雅人, 柳田隆正, 塩竈和也, 大熊真人, 芳本信子, 宮地栄一

形態・機能, 17(1) 40-40, Aug, 2018
虹彩由来iPS細胞を用いた水晶体上皮細胞への分化誘導

山本直樹, 平松範子, 平松範子, 大熊真人, 磯谷澄都, 谷川篤宏, 今泉和良, 宮地栄一, 堀口正之

日本臨床分子形態学会総会・学術集会講演プログラム・要旨集, 50th 101, Aug, 2018
スナネズミに松葉エキス投与後における抗酸化作用

金子千之, 加藤好光, 藤田公和, 日高聰, 安倍雅人, 柳田隆正, 塩竈和也, 大熊真人, 芳本信子, 宮地栄一

生物試料分析, 41(1) 43-43, Feb 23, 2018
マウス胚性幹細胞由来AVP神経の電気生理学的特性

金子葉子, 大熊真人, 小谷侑, 須賀英隆, 中島昭, 宮地栄一, 長崎弘

日本神経内分泌学会学術集会プログラム・抄録集, 44th(4) 43-1227, Dec, 2017
意見プレゼンテーション評価シートの導入による学生発表会の改善

中島昭, 近藤一直, 宮地栄一, 飯塚成志, 池本和久, 石原悟, 大熊真人, 金子葉子, 河合房夫, 小谷侑 …

医学教育, 48(5) 323-325, Oct, 2017
Immunohistochemical and physiological analyses of histamine receptors on ganglion cells in the developing gerbil retina

E. Miyachi, H. Imada, M. Ohkuma, K. Sakai

JOURNAL OF NEUROCHEMISTRY, 142 256-256, Aug, 2017
虹彩由来iPS細胞を用いた神経細胞・神経節細胞への分化誘導

山本直樹, 大熊真人, 平松範子, 磯谷澄都, 今泉和良, 谷川篤宏, 宮地栄一, 堀口正之

日本臨床分子形態学会総会・学術集会講演プログラム・要旨集, 49th 95, Aug, 2017
ヒト虹彩由来細胞を用いたiPS細胞作出と生理的機能を有する神経細胞への分化誘導

山本直樹, 大熊真人, 平松範子, 磯谷澄都, 今泉和良, 谷川篤宏, 宮地栄一, 堀口正之

組織培養研究, 36(3) 111-111, May, 2017
虹彩由来細胞を用いたiPS細胞作出と生理的機能を有する神経細胞への分化誘導

山本直樹, 大熊真人, 平松範子, 磯谷澄都, 今泉和良, 谷川篤宏, 宮地栄一, 堀口正之

日本臨床分子形態学会総会・学術集会講演プログラム・要旨集, 48th 86, Aug, 2016
Expression of histamine receptors in the gerbil retinal neurons

E. Miyachi, H. Imada, M. Ohkuma, K. Sakai

ACTA OPHTHALMOLOGICA, 93, Oct, 2015
虹彩由来網膜幹/前駆細胞の維持培養と分化細胞の生理学的機能評価

山本直樹, 大熊真人, 平松範子, 谷川篤宏, 堀口正之, 谷口孝喜, 宮地栄一

日本臨床分子形態学会総会・学術集会講演プログラム・要旨集, 45th 93, 2013
分化誘導した虹彩由来網膜幹/前駆細胞の生理学的機能評価

山本直樹, 大熊真人, 谷川篤宏, 堀口正之, 宮地栄一

再生医療, 11 171, May 16, 2012
スナネズミ網膜神経節細胞におけるヒスタミンの影響

大熊真人, 今田英己, 宮地栄一

日本生理学雑誌, 73(5) 139-140, May 1, 2011
虹彩由来網膜幹/前駆細胞の分化細胞における生理学的機能評価

山本直樹, 大熊真人, 谷川篤宏, 堀口正之, 宮地栄一

日本臨床分子形態学会総会・学術集会講演プログラム・要旨集, 43rd 60, 2011
スナネズミ網膜におけるヒスタミンによる電位依存性チャネルの修飾(Histamine modulates membrane currents in retinal ganglion cells of the gerbil)

大熊真人, 今田英己, 宮地栄一

神経化学, 49(2-3) 551-551, Aug, 2010
嗅上皮におけるTRPV1の生理学的機能についての研究

三村英也, 伊藤周史, 堀部晴司, 内藤健晴, 今田英己, 大熊真人, 宮地栄一

日本鼻科学会会誌, 49(3) 430-430, Jul 30, 2010
脂肪組織幹細胞を用いた神経細胞への分化誘導

山本直樹, 赤松浩彦, 長谷川靖司, 山田貴亮, 中田悟, 大熊真人, 宮地栄一, 丸野内棣, 松永佳世子

組織培養研究, 27(1) 76-76, Mar 31, 2008
APCは後シナプスへのPSD-95とAMPA受容体のクラスタリングを促進する

下村敦司, 大熊真人, 向後晶子, 野村隆士, 宮地栄一, 千田隆夫

藤田学園医学会誌, 32(1) 1-5, 2008
ラット嗅細胞におけるヒスタミン受容体の検討

三村英也, 伊藤周史, 齋藤正治, 今田英己, 大熊真人, 河合房夫, 内藤健晴, 宮地栄一

耳鼻咽喉科免疫アレルギー, 25(2 CD-ROM) ROMBUNNO.60-163, Sep, 2007
Distribution of histamine recepter in the gerbil retina

Imada Hideki, Kokubo Masahiro, Kato Toshi-ake, Ohkuma Mahito, Miyachi Ei-ichi

Proceedings of Annual Meeting of the Physiological Society of Japan, 2007 110-110, 2007

Books and Other Publications

Suppression by odorants of voltage-gated and ligand-gated channels in refinal neurons.

Spriner-Verlag, The Neural Basis of Early Vision, 2003
Suppression by odorants of voltage-gated and ligand-gated channels in refinal neurons.

Spriner-Verlag, The Neural Basis of Early Vision, 2003
The biology of ascidians: Light regulated GnRH neurons in biological clock for reproduction in the ascidian, Halocynthia roretzi.

Springer-Verlag, 2001
The biology of ascidians: Light regulated GnRH neurons in biological clock for reproduction in the ascidian, Halocynthia roretzi.

Springer-Verlag, 2001
ホヤの生物学 12章脳で感じる光感覚

東京大学出版会, 1998

Professional Memberships

Research Projects

ゲノム編集技術を駆使したミトコンドリアDNA変異iPS細胞病態モデルの確立

科学研究費助成事業, 基盤研究(C), 日本学術振興会, Apr, 2021 - Mar, 2024

八幡直樹, 大熊真人, 中嶋和紀
遊離Ｄ-グルタミン蓄積による網膜神経細胞および神経回路修飾の生理学的解析

科学研究費助成事業, 基盤研究(C), 日本学術振興会, Apr, 2020 - Mar, 2024

大熊真人
Development of three-dimensional retinal regeneration with electrophysiological function and drug safety evaluation using the human iris derived iPS cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
Differentiation to the functional retina cell using tissue stem/progenitor cell and iPS cell derived from human iris tissue.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2017 - Mar, 2020

Yamamoto Naoki
Fundamental medical research of the retinal regeneration using tissue stem / progenitor cells, immortalized cells, induced pluripotent stem cells derived from iris tissue.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017

Naoki Yamamoto, IMAIZUMI Kazuyosi, ISOGAI Sumito, HIRAMATSU Noriko, YAMASHITA Hiromi, YAMADA Harumoto, TAJIMA Kaori
The establishment of the effective culture method of retinal stem / progenitor cells derived from iris tissue and the physiologic function test of differentiated cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2010 - 2012

YAMAMOTO Naoki, TANIKAWA Atsuhiro, OHKUMA Mahito, HORIGUCHI Masayuki, MIYACHI Ei-ichi, SENDA Takao
Functional analyses of ion-channels and receptors in adult and developing mammalian sensory neurons.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2008 - 2010

MIYACHI Ei-ichi, OHKUMA Mahito, IMADA Hideki
Novel C-terminus-specific functions of the APC protein

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2008 - 2010

SENDA Takao, NOMURA Ryuji, SHIMOMURA Atsushi, HASEGAWA Yoshimi, KOGO Akiko, OHKUMA Mahito, MIYAKAWA Tsuyoshi, MIYACHI Ei-Ichi, ONOUCHI Takanori
Analyses of the physiological functions of voltage-dependent ion channels in sensory organs and their expressions in the process of regeneration and differentiation in sensory organs.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2005 - 2006

MIYACHI Eiichi, KAWAI Fusao, OHKUMA Mahito, IMADA Hideki, WATANABE Masami
感覚情報処理におけるヒスタミンの影響

2005
Histamine affects sensory signals in the retina and the olfactory epithelium.

2005
Physiological analyses of voltage-gated ion channels and their molecular identifications in sensory neurons.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2002 - 2003

MIYACHI Ei-ichi, HORIGUCHI Masayuki, OHKUMA Ahito, KAWAI Fusao
網膜細胞におけるイオンチャネルの研究

2001
Patch-clamp recording of retinal cells.

2001
神経伝達物質が嗅細胞の電位依存性電流に及ぼす効果

2000
Effect of neurotransmitter for vottage-dependent currents in olfactory receptor cell.

2000

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