2010年4月
Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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- 巻
- 109
- 号
- 4
- 開始ページ
- 407
- 終了ページ
- 410
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jbiosc.2009.09.050
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression. (C) 2009, The Society for Biotechnology, japan. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.jbiosc.2009.09.050
- ISSN : 1389-1723
- Web of Science ID : WOS:000278114100018