論文

査読有り
2016年2月

The Effect of D-Aspartate on Spermatogenesis in Mouse Testis

BIOLOGY OF REPRODUCTION
  • Keiji Tomita
  • ,
  • Hiroyuki Tanaka
  • ,
  • Susumu Kageyama
  • ,
  • Masayuki Nagasawa
  • ,
  • Akinori Wada
  • ,
  • Ryosuke Murai
  • ,
  • Kenichi Kobayashi
  • ,
  • Eiki Hanada
  • ,
  • Yasutoshi Agata
  • ,
  • Akihiro Kawauchi

94
2
開始ページ
30
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1095/biolreprod.115.134692
出版者・発行元
SOC STUDY REPRODUCTION

Spermatogenesis is controlled by hormonal secretions from the hypothalamus and pituitary gland, by factors produced locally in the testis, and by direct interaction between germ cells and Sertoli cells in seminiferous tubules. Although the mammalian testis contains high levels of D-aspartate (D-Asp), and D-Asp is known to stimulate the secretion of testosterone in cultured Leydig cells, its role in testis is unclear. We describe here biochemical, immunohistochemical, and flow cytometric studies designed to elucidate developmental changes in testicular D-Asp levels and the direct effect of D-Asp on germ cells. We found that the concentration of D-Asp in mouse testis increased with growth and that fluctuations in D-Asp levels were controlled in part by its degradative enzyme, D-aspartate oxidase expressed in Sertoli cells. In vitro sperm production studies showed that mitosis in premeiotic germ cells was strongly inhibited by the addition of D-Asp to the culture medium. Moreover, immunohistochemical analysis demonstrated that D-Asp accumulated in the differentiated spermatids, indicating either transport of D-Asp to spermatids or its de novo synthesis in these cells. Such compartmentation seems to prevent premeiotic germ cells in mouse testis from being exposed to the excess amount of D-Asp. In concert, our results indicate that in mouse testis, levels of D-Asp are regulated in a spatiotemporal manner and that D-Asp functions as a modulator of spermatogenesis.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1095/biolreprod.115.134692
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26658710
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000370003800009&DestApp=WOS_CPL

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