Shinmura, Shigeto

J-GLOBAL         Last updated: Aug 24, 2019 at 02:55
 
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Name
Shinmura, Shigeto
URL
http://shimmura.net/top/
Affiliation
Keio University
Section
School of Medicine Department of Ophthalmology
Job title
Associate Professor
Research funding number
00235780

Research Areas

 
 

Education

 
Mar 1989
   
 
医学部, Keio University
 

Awards & Honors

 
May 2010
American Academy of Ophthalmology Achievement Award
 
Feb 2004
日本角膜学会学術奨励賞
 
Jul 2003
東京歯科大学学長奨励研究賞
 
Nov 2000
三四会奨励賞
 

Published Papers

 
Deng S., Borderie V., Chan C., Dana R., Figueiredo F., Gomes J., Pellegrini G., Shimmura S., Kruse F.
Cornea   38(3) 364-375   Mar 2019
© 2018 Wolters Kluwer Health, Inc. All rights reserved. Purpose: Despite extensive knowledge gained over the last 3 decades regarding limbal stem cell deficiency (LSCD), the disease is not clearly defined, and there is lack of agreement on the dia...
Yamashita K., Hatou S., Uchino Y., Tsubota K., Shimmura S.
Japanese Journal of Ophthalmology   63(1) 56-64   Jan 2019
© 2018, Japanese Ophthalmological Society. Purpose: To evaluate the safety and efficacy of lamellar keratoplasty using preserved donor corneas to treat limbal dermoids. Study design: Retrospective study. Methods: The clinical records of 19 patient...
Fujii S., Yoshida S., Inagaki E., Hatou S., Tsubota K., Takahashi M., Shimmura S., Sugita S.
Stem Cells and Development   28(1) 28-43   Jan 2019
© Shota Fujii et al. 2018; Published by Mary Ann Liebert, Inc. 2018. Collecting sufficient quantities of primary neural crest cells (NCCs) for experiments is difficult, as NCCs are embryonic transient tissue that basically does not proliferate. We...
Venkatraman A., Hochart G., Bonnel D., Stauber J., Shimmura S., Rajamani L., Pervushin K., Mehta J.
Proteomics - Clinical Applications   13(1)    Jan 2019
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Scope: The purpose of this study is to identify and visualize the spatial distribution of proteins present in amyloid corneal deposits of TGFBI-CD patients using Mass Spectrometry Imaging (MSI) a...
Yamashita K., Hatou S., Inagaki E., Higa K., Tsubota K., Shimmura S.
Scientific Reports   8(1)    Dec 2018
© 2018, The Author(s). Unlike humans, rabbit corneal endothelial wounds are known to spontaneously heal. The current study was aimed to develop a new rabbit bullous keratopathy model using corneal endothelial cells that were induced to undergo end...
Yamashita, K., Inagaki, E., Hatou, S., Higa, K., Ogawa, A., Miyashita, H., Tsubota, K. and Shimmura, S.
Stem Cells Dev   27(16) 1097-1108   Jul 2018
Corneal blindness is the third leading cause of blindness in the world, and one of the main etiologies is dysfunction of the corneal endothelium. Current treatment of corneal endothelial disease is allogenic corneal transplantation, which is limit...
Den, S., Shimmura, S. and Shimazaki, J.
J Cataract Refract Surg   44(4) 496-503   Apr 2018
PURPOSE: To assess the efficacy and safety of cataract surgery after deep anterior lamellar keratoplasty (DALK) and penetrating keratoplasty (PKP). SETTING: Tokyo Dental College Ichikawa General Hospital, Chiba, Japan. DESIGN: Retrospective case s...
Yamane, M., Ogawa, Y., Mukai, S., Yaguchi, S., Kamijuku, H., Inaba, T., Asai, K., Morikawa, S., Kawakami, Y., Shimmura, S. and Tsubota, K.
Cornea   37(1) 102-108   Jan 2018
PURPOSE: This study aimed to clarify the mechanisms and assess the characteristics of the chronic graft-versus-host disease (cGVHD) fibrosis in the lacrimal gland (LG) of mice. METHODS: Histopathology of LG tissues was examined by immunohistochemi...
Yamazaki, R., Yamazoe, K., Yoshida, S., Hatou, S., Inagaki, E., Okano, H., Tsubota, K. and Shimmura, S.
Sci Rep   7(1) 15584   Nov 2017
Dry eye disease (DED) is a common disorder causing discomfort and ocular fatigue. Corneal nerves are compromised in DED, which may further cause loss of corneal sensation and decreased tear secretion. Semaphorin 3A (Sema3A) is expressed by the cor...
Inagaki, E., Hatou, S., Higa, K., Yoshida, S., Shibata, S., Okano, H., Tsubota, K. and Shimmura, S.
Stem Cells Transl Med   6(3) 788-798   Mar 2017
Corneal blindness is the fourth leading cause of blindness in the world. Current treatment is allogenic corneal transplantation, which is limited by shortage of donors and immunological rejection. Skin-derived precursors (SKPs) are postnatal stem ...
Shimmura-Tomita, M., Shimmura, S., Tsubota, K. and Shimazaki, J.
J Surg Educ   74(2) 258-263   Mar 2017
BACKGROUND: Reports on keratoplasty by residents are rare compared to those on cataract surgery. The aim of this study was to compare the results and surgery time of penetrating keratoplasty performed by residents and an experienced cornea surgeon...
Miyashita, H., Niwano, H., Yoshida, S., Hatou, S., Inagaki, E., Tsubota, K. and Shimmura, S.
Sci Rep   7 43557   Feb 2017
Cultures of epithelial cells are limited by the proliferative capacity of primary cells and cell senescence. Herein we show that primary human epithelial cell sheets cultured without dermal equivalents maintained homeostasis in vitro for at least ...
Hirayama, M., Ko, S. B. H., Kawakita, T., Akiyama, T., Goparaju, S. K., Soma, A., Nakatake, Y., Sakota, M., Chikazawa-Nohtomi, N., Shimmura, S., Tsubota, K. and Ko, M. S. H.
NPJ Aging Mech Dis   3 1   2017
Dry eye disease is the most prevalent pathological condition in aging eyes. One potential therapeutic strategy is the transplantation of lacrimal glands, generated in vitro from pluripotent stem cells such as human embryonic stem cells, into patie...
Ishiyama, S., Mori, Y., Nejima, R., Tokudome, T., Shimmura, S., Miyata, K. and Amano, S.
Cornea      Aug 2016
PURPOSE: To compare the long-term outcomes of visual function and endothelial cell survival after Descemet stripping automated endothelial keratoplasty (DSAEK) and penetrating keratoplasty (PK). METHODS: The cohort included 108 eyes from 97 patien...
Hirayama, M., Liu, Y., Kawakita, T., Shimmura, S. and Tsubota, K.
Exp Eye Res   146 54-9   May 2016
PURPOSE: The lacrimal gland secretes tear fluids that protect the ocular surface epithelium, and its dysfunction leads to dry eye disease (DED). The functional restoration of the lacrimal gland by engraftment of a bioengineered lacrimal gland usin...
Hirayama, M., Kawakita, T., Tsubota, K. and Shimmura, S.
Ocul Surf   14(2) 135-43   Apr 2016
The lacrimal gland produces the aqueous component of tears, including electrolytes, peptides, and glycoproteins necessary to maintain homeostasis and optical properties of the ocular surface. Stem cells that contribute to the homeostasis of the la...
Ogawa, A., Matsumoto, Y., Yaguchi, T., Shimmura, S. and Tsubota, K.
J Infect Chemother   22(4) 257-60   Apr 2016
We describe a 66-year-old woman who suffered from fungal keratitis after corneal transplantation. The causative organism was identified as Beauveria bassiana on the basis of morphological characteristics and the sequence of the internal transcribe...
Ogawa, Y., Morikawa, S., Okano, H., Mabuchi, Y., Suzuki, S., Yaguchi, T., Sato, Y., Mukai, S., Yaguchi, S., Inaba, T., Okamoto, S., Kawakami, Y., Tsubota, K., Matsuzaki, Y. and Shimmura, S.
Elife   5 e09394   Jan 2016
Fibrosis of organs is observed in systemic autoimmune disease. Using a scleroderma mouse, we show that transplantation of MHC compatible, minor antigen mismatched bone marrow stromal/stem cells (BMSCs) play a role in the pathogenesis of fibrosis. ...
Yaguchi, S., Ogawa, Y., Kawakita, T., Shimmura, S. and Tsubota, K.
Cornea   34 Suppl 11 S142-52   Nov 2015
Chronic graft-versus-host disease (cGVHD) is a serious complication known to occur after allogeneic hematopoietic stem cell transplantation. Clinical manifestation includes inflammation and fibrosis. Many peripheral tissues are capable of generati...
Fukui, M., Ogawa, Y., Shimmura, S., Hatou, S., Ichihashi, Y., Yaguchi, S., Hirayama, M., Kawakita, T. and Tsubota, K.
Mod Rheumatol   1-7   Apr 2015
OBJECTIVE: Immunoglobulin G4 (IgG4)-related Mikulicz's disease (MD) is a fibrosis-associated inflammatory disease, often accompanied by lacrimal gland swelling. Although much attention has been paid to the inflammatory aspects of this disease, the...
Kawakita, T., Shimmura, S. and Tsubota, K.
Asia Pac J Ophthalmol (Phila)   4(2) 101-5   Mar 2015
PURPOSE: The aim of this study is to evaluate the efficacy and safety of oral pilocarpine in treating severe dry eye unresponsive to conventional conservative treatment in patients with Sjogren syndrome. DESIGN: A prospective study. METHODS: Oral ...
Kamoshita, M., Matsumoto, Y., Nishimura, K., Katono, Y., Murata, M., Ozawa, Y., Shimmura, S. and Tsubota, K.
J Infect Chemother   21(2) 141-3   Feb 2015
We describe a 91-year-old woman who suffered from fungal keratitis after corneal transplantation. The causative organism was identified as Wickerhamomyces anomalus (formerly Pichia anomala or Hansenula anomala) on the basis of morphological charac...
Yamazoe, K., Yoshida, S., Yasuda, M., Hatou, S., Inagaki, E., Ogawa, Y., Tsubota, K. and Shimmura, S.
PLoS One   10(7) e0133397   2015
PURPOSE: To investigate the phenotype and predisposing factors of a granular corneal dystrophy type 2 transgenic mouse model. METHODS: Human TGFBI cDNA with R124H mutation was used to make a transgenic mouse expressing human protein (TGFBIR124H mo...
Fujii, S., Matsumoto, Y., Fukui, M., Fujitake, J., Kawakita, T., Shimmura, S. and Tsubota, K.
Cornea   33(12) 1320-3   Dec 2014
PURPOSE: To report that spontaneous postkeratoplasty wound dehiscence or gaps caused by suture removal may occur years after penetrating and deep lamellar keratoplasty. METHODS: We retrospectively reviewed the medical records of 667 keratoplasty p...
Satake, Y., Yamaguchi, T., Hirayama, M., Higa, K., Shimazaki-Den, S., Dogru, M., Kawakita, T., Kawashima, M., Shimmura, S., Tsubota, K. and Shimazaki, J.
Cornea   33 Suppl 11 S42-6   Nov 2014
: Recent advances in ocular surface reconstruction for patients with severe ocular surface diseases have significantly improved the prognosis of patients with vision-impairing corneal abnormalities. The history of cultivated epithelial sheet trans...
Kamoshita, M., Ozawa, Y., Kubota, S., Miyake, S., Tsuda, C., Nagai, N., Yuki, K., Shimmura, S., Umezawa, K. and Tsubota, K.
PLoS One   9(7) e103013   2014
Recent progress in molecular analysis has revealed the possible involvement of multiple inflammatory signaling pathways in pathogenesis of retinal degeneration. However, how aberrant signaling pathways cause tissue damage and dysfunction is still ...
Shiba, D., Hatou, S., Ono, T., Hosoda, S., Tanabe, S., Ozeki, N., Yuki, K., Shimoyama, M., Fukagawa, K., Shimmura, S. and Tsubota, K.
PLoS One   9(8) e104126   2014
PURPOSE: To design a mathematical model that can predict the relationship between the ganglion cell complex (GCC) thickness and visual field sensitivity (VFS) in glaucoma patients. DESIGN: Retrospective cross-sectional case series. METHOD: Within ...
Yoshida, T., Ozawa, Y., Suzuki, K., Yuki, K., Ohyama, M., Akamatsu, W., Matsuzaki, Y., Shimmura, S., Mitani, K., Tsubota, K. and Okano, H.
Mol Brain   7 45   2014
BACKGROUND: Retinitis pigmentosa (RP) is an inherited human retinal disorder that causes progressive photoreceptor cell loss, leading to severe vision impairment or blindness. However, no effective therapy has been established to date. Although ge...
Hatou, S., Higa, K., Inagaki, E., Yoshida, S., Kimura, E., Hayashi, R., Tsujikawa, M., Tsubota, K., Nishida, K. and Shimmura, S.
Tissue Eng Part C Methods   19(12) 901-10   Dec 2013
Tissue-engineering approaches to cultivate corneal endothelial cells (CECs) or induce CECs from stem cells are under investigation for the treatment of endothelial dysfunction. Before clinical application, a validation method to determine the qual...
Inagaki, E., Hatou, S., Yoshida, S., Miyashita, H., Tsubota, K. and Shimmura, S.
Invest Ophthalmol Vis Sci   54(12) 7258-65   Nov 2013
PURPOSE: To investigate the expression pattern of claudins in human corneal endothelium, and to evaluate the functional role of the claudin-10b subtype. METHODS: Corneal endothelium with Descemet's membrane and the corneal epithelium were stripped...
Miyashita, H., Yokoo, S., Yoshida, S., Kawakita, T., Yamagami, S., Tsubota, K. and Shimmura, S.
Stem Cells Transl Med   2(10) 758-65   Oct 2013
Corneal epithelial stem cells are located in the limbus, the junction between the cornea and the conjunctiva. A limbal epithelium model in vitro would be useful for the study of epithelial stem cells, as well as improving the quality of cultivated...
Konomi, K., Satake, Y., Shimmura, S., Tsubota, K. and Shimazaki, J.
Cornea   32(8) 1110-5   Aug 2013
PURPOSE: To evaluate the long-term efficacy of amniotic membrane transplantation as a treatment option in cases with partial limbal deficiency. METHODS: Sixteen eyes of 14 patients with partial limbal deficiency underwent amniotic membrane patch (...
Narimatsu, T., Ozawa, Y., Miyake, S., Kubota, S., Hirasawa, M., Nagai, N., Shimmura, S. and Tsubota, K.
Invest Ophthalmol Vis Sci   54(7) 4555-62   Jul 2013
PURPOSE: To elucidate the influences of light exposure on the retinal pigment epithelium (RPE) in vivo that may be involved in the pathogenesis of AMD. METHODS: Six- to 7-week-old BALB/c mice were exposed to light at 2000 lux for 3 hours. Flat-mou...
Hatou, S., Yoshida, S., Higa, K., Miyashita, H., Inagaki, E., Okano, H., Tsubota, K. and Shimmura, S.
Stem Cells Dev   22(5) 828-39   Mar 2013
Corneal endothelial dysfunction remains a major indication for corneal transplantation. Both corneal endothelial cells and stromal cells originate from the neural crest, but have distinct phenotypes and function in the adult cornea. We previously ...
Higa, K., Kato, N., Yoshida, S., Ogawa, Y., Shimazaki, J., Tsubota, K. and Shimmura, S.
Stem Cell Res   10(2) 147-55   Mar 2013
Stem cells have a specialized microenvironment for maintaining self-renewal and multipotent capacities. It is believed that a cornea epithelial stem cell niche exists in the limbus. To characterize the niche of limbal epithelial stem cells, we obs...
Hirayama, M., Ogawa, M., Oshima, M., Sekine, Y., Ishida, K., Yamashita, K., Ikeda, K., Shimmura, S., Kawakita, T., Tsubota, K. and Tsuji, T.
Nat Commun   4 2497   2013
The lacrimal gland has a multifaceted role in maintaining a homeostatic microenvironment for a healthy ocular surface via tear secretion. Dry-eye disease, which is caused by lacrimal gland dysfunction, is one of the most prevalent eye diseases tha...
Kawai, M., Ogawa, Y., Shimmura, S., Ohta, S., Suzuki, T., Kawamura, N., Kuwana, M., Kawakami, Y. and Tsubota, K.
Sci Rep   3 2455   2013
Aging is commonly defined as the accumulation of diverse deleterious changes in cells and tissues with advancing age. To investigate whether aging changes are involved in the lacrimal glands of chronic graft-versus-host disease (cGVHD) model mice,...
Kawakita, T., Kawabata, F., Tsuji, T., Kawashima, M., Shimmura, S. and Tsubota, K.
Biomed Res   34(5) 215-20   2013
The purpose of this study was to evaluate the efficacy of fish oil supplementation added to usual dry eye treatment in dry eye subjects in a randomized controlled trial. Twenty-seven typical dry eye subjects were selected from 43 candidates by the...
Yaguchi, S., Ogawa, Y., Shimmura, S., Kawakita, T., Hatou, S., Satofuka, S., Nakamura, S., Imada, T., Miyashita, H., Yoshida, S., Yaguchi, T., Ozawa, Y., Mori, T., Okamoto, S., Kawakami, Y., Ishida, S. and Tsubota, K.
PLoS One   8(6) e64724   2013
Chronic graft-versus-host disease (cGVHD), a serious complication following allogeneic HSCT (hematopoietic stem cell transplantation), is characterized by systemic fibrosis. The tissue renin-angiotensin system (RAS) is involved in the fibrotic pat...
Ozeki, N., Yuki, K., Shiba, D., Shimmura, S., Murat, D. and Tsubota, K.
Jpn J Ophthalmol   56(4) 307-11   Jul 2012
PURPOSE: To investigate the incidence of intraocular pressure (IOP) elevation after Descemet's stripping endothelial keratoplasty (DSEK) and associated clinical findings. METHODS: Forty-eight eyes of 46 patients who underwent DSEK were studied in ...
Tatematsu, Y., Ogawa, Y., Shimmura, S., Dogru, M., Yaguchi, S., Nagai, T., Yamazaki, K., Kameyama, K., Okamoto, S., Kawakami, Y. and Tsubota, K.
Bone Marrow Transplant   47(3) 416-25   Mar 2012
The ocular surface is a frequent target tissue of mucosal chronic GVHD (cGVHD). We investigated the histopathological features of the conjunctival microvilli in patients with cGVHD. Conjunctival tissue specimens from patients with cGVHD or Sjogren...
Kamoi, M., Ogawa, Y., Nakamura, S., Dogru, M., Nagai, T., Obata, H., Ito, M., Kaido, M., Kawakita, T., Okada, Y., Kawakami, Y., Shimmura, S. and Tsubota, K.
PLoS One   7(9) e43688   2012
Previous observations in a rat model of a non-Sjogren's syndrome (non-SS) type of dry eye seen in users of visual display terminals (VDT) indicated that secretory vesicle (SV) accumulation in the lacrimal gland epithelia contributes to the conditi...
Kawashima, M., Kawakita, T., Inaba, T., Okada, N., Ito, M., Shimmura, S., Watanabe, M., Shinmura, K. and Tsubota, K.
PLoS One   7(3) e33148   2012
BACKGROUND: Decrease in lacrimal gland secretory function is related to age-induced dry eye disease. Lactoferrin, the main glycoprotein component of tears, has multiple functions, including anti-inflammatory effects and the promotion of cell growt...
Kobayashi, S., Kawakita, T., Kawashima, M., Okada, N., Mishima, K., Saito, I., Ito, M., Shimmura, S. and Tsubota, K.
Mol Vis   18 1271-7   2012
PURPOSE: To date, mouse lacrimal gland epithelial cells have been cultured successfully but only in cases involving newborn mouse lacrimal glands. In this work, we attempted to cultivate and characterize adult mouse lacrimal gland epithelial cells...
Omoto, M., Yoshida, S., Miyashita, H., Kawakita, T., Yoshida, K., Kishino, A., Kimura, T., Shibata, S., Tsubota, K., Okano, H. and Shimmura, S.
PLoS One   7(11) e47716   2012
BACKGROUND: Peripheral nerve damage of the cornea is a complication following surgery or infection which may lead to decreased visual function. We examined the efficacy of the semaphorin 3A inhibitor, SM-345431, in promoting regeneration of periph...
Uchino, Y., Kawakita, T., Miyazawa, M., Ishii, T., Onouchi, H., Yasuda, K., Ogawa, Y., Shimmura, S., Ishii, N. and Tsubota, K.
PLoS One   7(10) e45805   2012
Oxidative damage and inflammation are proposed to be involved in an age-related functional decline of exocrine glands. However, the molecular mechanism of how oxidative stress affects the secretory function of exocrine glands is unclear. We develo...
Yaguchi, S., Ogawa, Y., Shimmura, S., Hatou, S., Nakamura, S., Inaba, T., Imada, T., Ozawa, Y., Kawakami, Y., Ishida, S. and Tsubota, K.
Invest Ophthalmol Vis Sci   53(9) 5416-25   2012
PURPOSE: To investigate the expression, localization, and physiologic function of renin-angiotensin system (RAS) components in the mouse lacrimal gland. METHODS: Lacrimal glands and cultured lacrimal gland fibroblasts from wild-type (WT) BALB/c (H...
Higa, K., Takeshima, N., Moro, F., Kawakita, T., Kawashima, M., Demura, M., Shimazaki, J., Asakura, T., Tsubota, K. and Shimmura, S.
J Biomater Sci   22 2261-2276   Nov 2011
Biological carriers, such as the amniotic membrane and serum-derived fibrin, are currently used to deliver cultivated corneal epithelial sheets to the ocular surface. Such carriers require being transparent and allowing the diffusion of metabolite...
Kawakita, T., Higa, K., Shimmura, S., Tomita, M., Tsubota, K. and Shimazaki, J.
Invest Ophthalmol Vis Sci   52(11) 8132-7   Oct 2011
PURPOSE: To characterize corneal epithelial cells separated from limbus in vivo by transplantation of a stainless steel ring with or without creating a defect inside the ring. METHODS: A stainless steel ring (diameter, 8 mm; width, 300 mum; depth,...
Hatou, S., Dogru, M., Ibrahim, O. M., Wakamatsu, T., Sato, E. A., Shimmura, S., Negishi, K. and Tsubota, K.
Invest Ophthalmol Vis Sci   52(9) 6680-9   Aug 2011
PURPOSE: To evaluate the extent of corneal inflammation and the response to treatment in patients with Mooren's ulcer, by in vivo confocal microscopy (IVCM). METHODS: Twenty-two eyes of 15 patients with Mooren's ulcer were enrolled in this prospec...
Shimazaki, J., Den, S., Omoto, M., Satake, Y., Shimmura, S. and Tsubota, K.
Am J Ophthalmol   152(1) 33-39 e1   Jul 2011
PURPOSE: Immunologic rejection remains a major cause of graft failure in high-risk corneal transplantation. This study was conducted to elucidate the efficacy and safety of systemic cyclosporine (CsA) in high-risk corneal transplantation. DESIGN: ...
Okada, N., Kawakita, T., Mishima, K., Saito, I., Miyashita, H., Yoshida, S., Shimmura, S. and Tsubota, K.
Invest Ophthalmol Vis Sci   52(6) 2905-10   May 2011
PURPOSE: Although the cornea expresses high levels of clusterin (CLU), the role of CLU in the cornea is poorly understood. This study was performed to investigate the possible role of CLU in corneal epithelial homeostasis. METHODS: CLU was overexp...
Uchino, Y., Shimmura, S., Yamaguchi, T., Kawakita, T., Matsumoto, Y., Negishi, K. and Tsubota, K.
Cornea   30(3) 287-90   Mar 2011
PURPOSE: To compare corneal thickness and haze in Descemet stripping automated endothelial keratoplasty (DSAEK) and penetrating keratoplasty (PK). METHODS: This study included 30 consecutive patients undergoing DSAEK (18 patients) and PK (12 patie...
Suzuki, A., Matsumoto, Y., Uchino, Y., Kawakita, T., Shimmura, S. and Tsubota, K.
Nihon Ganka Gakkai Zasshi   115(2) 116-21   Feb 2011
PURPOSE: To evaluate the risk factors of severe peripheral corneal ulcers. PATIENTS AND METHODS: Twenty three eyes of 15 patients (9 eyes of 7 men and 14 eyes of 8 women) with severe peripheral corneal ulcers, who were treated during the past 10 y...
Kubota, M., Shimmura, S., Kubota, S., Miyashita, H., Kato, N., Noda, K., Ozawa, Y., Usui, T., Ishida, S., Umezawa, K., Kurihara, T. and Tsubota, K.
Invest Ophthalmol Vis Sci   52(1) 427-33   Jan 2011
PURPOSE: To investigate the role of reactive oxygen species (ROS) as the prime initiators of the angiogenic response after alkali injury of the cornea and observe the effects of antioxidants in preventing angiogenesis. METHODS: The corneal epithel...
Hatou, S., Shimmura, S., Shimazaki, J., Usui, T., Amano, S., Yokogawa, H., Kobayashi, A., Zheng, X., Shiraishi, A., Ohashi, Y., Inatomi, T. and Tsubota, K.
Invest Ophthalmol Vis Sci   52(11) 7888-93   2011
PURPOSE. To devise a mathematical disease classification model for eyes with primary guttata cornea, on the bases of endothelial loss trajectory and probability of advanced disease. METHODS. A series of 1971 patients (3281 eyes), some with and som...
Inagaki, E., Ogawa, Y., Matsumoto, Y., Kawakita, T., Shimmura, S. and Tsubota, K.
Mol Vis   17 598-606   2011
PURPOSE: To report the clinical features and investigate the underlying pathological processes of spontaneous corneal perforation in patients with ocular chronic graft-versus-host disease (cGVHD). METHODS: A full ophthalmological evaluation of cor...
Kawashima, M., Kawakita, T., Maida, Y., Kamoi, M., Ogawa, Y., Shimmura, S., Masutomi, K. and Tsubota, K.
Mol Vis   17 1397-404   2011
PURPOSE: Indicators of aging such as disruption of telomeric function due to shortening may be more frequent in dysfunctional lacrimal gland. The aims of this study were to 1) determine the viability of quantitative fluorescence in situ hybridizat...
Niibe, K., Kawamura, Y., Araki, D., Morikawa, S., Miura, K., Suzuki, S., Shimmura, S., Sunabori, T., Mabuchi, Y., Nagai, Y., Nakagawa, T., Okano, H. and Matsuzaki, Y.
PLoS One   6(3) e17610   2011
BACKGROUND: Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by the forced expression of defined transcription factors. Although most somatic cells are capable of acquiring pluripotency with minimal gene transd...
Shimmura, S. and Hatou, S.
Invest Ophthalmol Vis Sci   52(13) 9488   2011
Yoshida, S., Yasuda, M., Miyashita, H., Ogawa, Y., Yoshida, T., Matsuzaki, Y., Tsubota, K., Okano, H. and Shimmura, S.
PLoS One   6(12) e28856   2011
BACKGROUND: Application of induced pluripotent stem (iPS) cells in regenerative medicine will bypass ethical issues associated with use of embryonic stem cells. In addition, patient-specific IPS cells can be useful to elucidate the pathophysiology...
Kubota, M., Shimmura, S., Miyashita, H., Kawashima, M., Kawakita, T. and Tsubota, K.
Invest Ophthalmol Vis Sci   51(11) 5617-22   Nov 2010
PURPOSE: ABCG2 is a putative marker of progenitor cells, including the corneal epithelium. The authors investigated whether ABCG2 functions in the homeostasis of corneal epithelial cells using abcg2 knockout (KO) mice and corneal epithelial cell l...
Omoto, M., Shimmura, S., Hatou, S., Ichihashi, Y., Kawakita, T. and Tsubota, K.
Jpn J Ophthalmol   54(6) 537-43   Nov 2010
PURPOSE: To report the efficacy of simultaneous keratolimbal allograft (KLAL) surgery and deep anterior lamellar keratoplasty (DALK) for limbal stem cell deficiency (LSCD). METHODS: We conducted a retrospective, interventional case series of six c...
Yamaguchi, T., Negishi, K., Yamaguchi, K., Dogru, M., Uchino, Y., Shimmura, S. and Tsubota, K.
Cornea   29(10) 1086-90   Oct 2010
PURPOSE: To evaluate the irregularity of the anterior and posterior cornea after Descemet stripping automated endothelial keratoplasty (DSAEK) and penetrating keratoplasty (PK). METHODS: This clinical study comprised 39 eyes: 13 consecutive eyes a...
Kawashima, M., Kawakita, T., Okada, N., Ogawa, Y., Murat, D., Nakamura, S., Nakashima, H., Shimmura, S., Shinmura, K. and Tsubota, K.
Biochem Biophys Res Commun   397(4) 724-8   Jul 2010
A decrease in lacrimal gland secretory function is closely related to aging and leads to an increased prevalence of dry eye syndrome. Since calorie restriction (CR) is considered to prevent functional decline of various organs due to aging, we hyp...
Ogawa, Y., Shimmura, S., Kawakita, T., Yoshida, S., Kawakami, Y. and Tsubota, K.
Am J Pathol   175(6) 2372-81   Dec 2009
Chronic graft-versus-host disease (cGVHD) of the ocular surface and lacrimal gland is a vision-threatening condition that occurs after allogeneic bone marrow transplantation. In this study, we used immunohistochemistry and electron microscopy to i...
Kawashima, M., Kawakita, T., Shimmura, S., Tsubota, K. and Shimazaki, J.
Ophthalmology   116(11) 2072-6   Nov 2009
OBJECTIVE: To analyze risk factors of traumatic wound rupture after penetrating keratoplasty (PKP) or deep anterior lamellar keratoplasty (DALK). DESIGN: Retrospective case series. PARTICIPANTS: A total of 1962 consecutive cases were included. MET...
Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H. and Matsuzaki, Y.
J Exp Med   206(11) 2483-96   Oct 2009
Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identifie...
Higa, K., Shimmura, S., Miyashita, H., Kato, N., Ogawa, Y., Kawakita, T., Shimazaki, J. and Tsubota, K.
Invest Ophthalmol Vis Sci   50(10) 4640-5   Oct 2009
PURPOSE: To demonstrate the role of N-cadherin (N-cad) in maintaining the progenitor status of primary human limbal epithelial cells in vitro. METHODS: Immunohistochemistry and immunoelectron microscopy against N-cad was performed in human limbal ...
Kawakita, T., Shimmura, S., Higa, K., Espana, E. M., He, H., Shimazaki, J., Tsubota, K. and Tseng, S. C.
Invest Ophthalmol Vis Sci   50(10) 4611-7   Oct 2009
PURPOSE: To determine the growth potential of p63-positive cell clusters maintained in human limbal epithelial sheets. METHODS: Intact human limbal epithelial sheets were isolated from corneoscleral rims and cultured with or without being rendered...
Ma, X., Shimmura, S., Miyashita, H., Yoshida, S., Kubota, M., Kawakita, T. and Tsubota, K.
Invest Ophthalmol Vis Sci   50(6) 2716-21   Jun 2009
PURPOSE: To develop a reproducible procedure for the long-term culture of corneal epithelial cells from a single mouse cornea. METHODS: Corneal limbal explants of C57BL6/J mice were cultured in serum-free, low-Ca(2+) medium supplemented with EGF a...
Omoto, M., Miyashita, H., Shimmura, S., Higa, K., Kawakita, T., Yoshida, S., McGrogan, M., Shimazaki, J. and Tsubota, K.
Invest Ophthalmol Vis Sci   50(5) 2109-15   May 2009
PURPOSE: To report the efficacy of human bone marrow-derived mesenchymal stem cells as a source of feeder cells for the cultivation of transplantable corneal epithelial cell sheets. METHODS: Human mesenchymal stem cells (marrow adherent stem cells...
Yamaguchi, T., Negishi, K., Yamaguchi, K., Murat, D., Uchino, Y., Shimmura, S. and Tsubota, K.
J Cataract Refract Surg   35(4) 688-94   Apr 2009
PURPOSE: To evaluate irregularity of the anterior and posterior cornea before and after Descemet-stripping endothelial keratoplasty (DSEK) and its effect on visual acuity. SETTING: Department of Ophthalmology, Keio University School of Medicine, T...
Morikawa, S., Mabuchi, Y., Niibe, K., Suzuki, S., Nagoshi, N., Sunabori, T., Shimmura, S., Nagai, Y., Nakagawa, T., Okano, H. and Matsuzaki, Y.
Biochem Biophys Res Commun   379(4) 1114-9   Feb 2009
Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as ''dediffer...
Yuki, K., Shimmura, S., Shiba, D. and Tsubota, K.
Jpn J Ophthalmol   53(1) 68-70   Jan 2009
Kawashima, M., Kawakita, T., Yoshida, S., Shimmura, S. and Tsubota, K.
Mol Vis   15 1162-8   2009
PURPOSE: Nucleostemin, a nuclear protein involved in the regulation of cell cycle and proliferation, is a candidate marker for various stem cells. We examined the expression of nucleostemin as a marker of differentiation and senescence in corneal ...
Kurihara, T., Omoto, M., Noda, K., Ebinuma, M., Kubota, S., Koizumi, H., Yoshida, S., Ozawa, Y., Shimmura, S., Ishida, S. and Tsubota, K.
Mol Vis   15 2751-61   2009
PURPOSE: To establish a novel murine intraocular lens (IOL) implantation model to study the protective effects of colored-IOLs against retinal phototoxicity. METHODS: Two-millimeter diameter IOL buttons were created from IOLs for clinical use. Ext...
Uchino, M., Dogru, M., Uchino, Y., Fukagawa, K., Shimmura, S., Takebayashi, T., Schaumberg, D. A. and Tsubota, K.
Am J Ophthalmol   146(6) 925-9 e2   Dec 2008
PURPOSE: To determine the prevalence of dry eye disease and contact lens (CL) use among Japanese private high school students. DESIGN: Cross-sectional prevalence survey. METHODS: We carried out a cross-sectional survey in 3,433 Japanese high schoo...
Uchino, M., Schaumberg, D. A., Dogru, M., Uchino, Y., Fukagawa, K., Shimmura, S., Satoh, T., Takebayashi, T. and Tsubota, K.
Ophthalmology   115(11) 1982-8   Nov 2008
OBJECTIVE: To determine the prevalence of dry eye disease (DED) and risk factors among young and middle-aged Japanese office workers using visual display terminals (VDTs). DESIGN: Cross-sectional prevalence survey. PARTICIPANTS: Four thousand thre...
Ozawa, Y., Nakao, K., Kurihara, T., Shimazaki, T., Shimmura, S., Ishida, S., Yoshimura, A., Tsubota, K. and Okano, H.
J Biol Chem   283(36) 24561-70   Sep 2008
Inflammatory cytokines cause tissue dysfunction. We previously reported that retinal inflammation down-regulates rhodopsin expression and impairs visual function by an unknown mechanism. Here, we demonstrate that rhodopsin levels were preserved by...
Yuki, K., Shimmura, S., Shiba, D. and Tsubota, K.
Br J Ophthalmol   92(9) 1299-300   Sep 2008
Kawakita, T., Shimmura, S., Hornia, A., Higa, K. and Tseng, S. C.
J Cell Mol Med   12(4) 1303-16   Aug 2008
The limbal region of the adult cornea contains stem cells which are ultimately responsible for regeneration of the corneal epithelium during wound repair. However, primarily-isolated murine corneal/limbal epithelial cells rapidly senesce on plasti...
Igarashi, T., Shimmura, S., Yoshida, S., Tonogi, M., Shinozaki, N. and Yamane, G. Y.
Oral Dis   14(5) 413-8   Jul 2008
OBJECTIVE: Although oral mucosal epithelial stem cells are thought to reside in the basal layer, such cells have not yet been isolated. We isolated a population of rabbit oral epithelial progenitor cells containing putative stem cells. MATERIALS A...
Miyashita, H., Shimmura, S., Higa, K., Yoshida, S., Kawakita, T., Shimazaki, J. and Tsubota, K.
Tissue Eng Part A   14(7) 1275-82   Jul 2008
Corneal epithelial cell sheets co-cultivated with feeder cells are used to reconstruct the ocular surface in stem cell-depleted eyes. The present study was conducted to investigate the optimal method of using feeder cells in the interest of preser...
Mochimaru, H., Usui, T., Yaguchi, T., Nagahama, Y., Hasegawa, G., Usui, Y., Shimmura, S., Tsubota, K., Amano, S., Kawakami, Y. and Ishida, S.
Invest Ophthalmol Vis Sci   49(5) 2172-7   May 2008
PURPOSE: To investigate whether the induction of cytotoxic T lymphocytes (CTLs) targeting VEGF receptor 2 inhibits corneal neovascularization caused by alkali injury. METHODS: H-2Db-restricted peptide corresponding to amino acids 400 to 408 of VEG...
Tomita, M., Shimmura, S., Tsubota, K. and Shimazaki, J.
Ophthalmology   115(5) 851-6   May 2008
PURPOSE: To estimate the incidence of and investigate possible risk factors for postkeratoplasty atopic sclerokeratitis in keratoconus patients undergoing keratoplasty. DESIGN: Retrospective, noncomparative, interventional case series. PARTICIPANT...
Dogru, M., Kato, N., Matsumoto, Y., Tanaka, Y., Akabane, N., Shimmura, S., Tsubota, K. and Shimazaki, J.
Curr Eye Res   32(10) 863-70   Oct 2007
Purpose: To describe the immunohistochemical and electron microscopic characteristics of retrocorneal scrolls in syphilitic interstitial keratitis. Methods: Five eyes of five patients with congenital syphilitic interstitial keratitis who underwent...
Miyashita, H., Higa, K., Kato, N., Kawakita, T., Yoshida, S., Tsubota, K. and Shimmura, S.
Invest Ophthalmol Vis Sci   48(8) 3586-93   Aug 2007
PURPOSE: To demonstrate the effects of hypoxia on proliferation and differentiation of human limbal epithelial cells in vitro. METHODS: Primary human limbal epithelial cells were harvested from the rim of donor corneas. Colony-forming efficiency (...
Shimazaki, J., Higa, K., Morito, F., Dogru, M., Kawakita, T., Satake, Y., Shimmura, S. and Tsubota, K.
Am J Ophthalmol   143(6) 945-53   Jun 2007
PURPOSE: To analyze factors influencing clinical outcomes in cultivated limbal epithelial transplantation (CLET). DESIGN: Retrospective, observational case series. METHODS: Twenty-seven eyes of 27 patients that had CLET for severe chronic cicatric...
Den, S., Shimmura, S., Tsubota, K. and Shimazaki, J.
Am J Ophthalmol   143(5) 750-4   May 2007
PURPOSE: To study the influence of the Descemet membrane (DM) perforations after deep lamellar keratoplasty (DLKP) on endothelial decompensation, endothelial density, visual acuity, and postoperative complications. The relationship between DM perf...
Kato, N., Shimmura, S., Kawakita, T., Miyashita, H., Ogawa, Y., Yoshida, S., Higa, K., Okano, H. and Tsubota, K.
Invest Ophthalmol Vis Sci   48(4) 1511-7   Apr 2007
PURPOSE: To investigate whether beta-catenin activation and epithelial-mesenchymal transition (EMT) is involved in the pathogenesis of pterygium. METHODS: beta-Catenin and E-cadherin expression were examined in surgically excised tissue and eye ba...
Uchino, Y., Shimmura, S., Miyashita, H., Taguchi, T., Kobayashi, H., Shimazaki, J., Tanaka, J. and Tsubota, K.
J Biomed Mater Res B Appl Biomater   81(1) 201-6   Apr 2007
Poly(vinyl alcohol) (PVA) is a biocompatible, transparent hydrogel with physical strength that makes it promising as a material for an artificial cornea. In our previous study, type I collagen was immobilized onto PVA (PVA-COL) as a possible artif...
Ozawa, Y., Nakao, K., Shimazaki, T., Shimmura, S., Kurihara, T., Ishida, S., Yoshimura, A., Tsubota, K. and Okano, H.
Dev Biol   303(2) 591-600   Mar 2007
Suppressor of cytokine signaling 3 (SOCS3) is an intracellular, ligand-induced negative feedback modulator of STAT3 activation that acts during inflammation. Here, we demonstrate that SOCS3 expression is important for normal retinal development in...
Ogawa, Y., Razzaque, M. S., Kameyama, K., Hasegawa, G., Shimmura, S., Kawai, M., Okamoto, S., Ikeda, Y., Tsubota, K., Kawakami, Y. and Kuwana, M.
Invest Ophthalmol Vis Sci   48(3) 1079-86   Mar 2007
PURPOSE: Uncontrolled fibrosis due to excessive accumulation of extracellular matrix proteins in the lacrimal glands of patients with chronic graft-versus-host disease (cGVHD) is well documented. Heat-shock protein 47 (HSP47) is involved in the mo...
Higa, K., Shimmura, S., Kato, N., Kawakita, T., Miyashita, H., Itabashi, Y., Fukuda, K., Shimazaki, J. and Tsubota, K.
Invest Ophthalmol Vis Sci   48(2) 597-604   Feb 2007
PURPOSE: To report a novel method of engineering transplantable, carrier-free corneal epithelial sheets by using a biodegradable fibrin sealant and to compare its characteristics with epithelial sheets cultivated on denuded amniotic membrane carri...
Higuchi, A., Ueno, R., Shimmura, S., Suematsu, M., Dogru, M. and Tsubota, K.
Curr Eye Res   32(2) 83-8   Feb 2007
PURPOSE: Because autologous serum is useful for the treatment of severe dry eye, serum components may be a potential candidate for the treatment of dry eye. Serum albumin is abundantly contained in human serum and plays many physiologic roles. We ...
Matsumoto, Y., Dogru, M., Sato, E. A., Katono, Y., Uchino, Y., Shimmura, S. and Tsubota, K.
Mol Vis   13 1319-26   2007
PURPOSE: To evaluate the role of in vivo laser scanning confocal microscopy (Heidelberg Retina Tomograph II, Rostock Cornea Module, HRTII-RCM) in the management of Acanthamoeba keratitis (AK). METHODS: Four eyes of four patients with AK seen at Ke...
Yoshida, S., Shimmura, S., Nagoshi, N., Fukuda, K., Matsuzaki, Y., Okano, H. and Tsubota, K.
Stem Cells   24(12) 2714-22   Dec 2006
We report the presence of neural crest-derived corneal precursors (COPs) that initiate spheres by clonal expansion from a single cell. COPs expressed the stem cell markers nestin, Notch1, Musashi-1, and ABCG2 and showed the side population cell ph...
Yoshida, S., Shimmura, S., Kawakita, T., Miyashita, H., Den, S., Shimazaki, J. and Tsubota, K.
Invest Ophthalmol Vis Sci   47(11) 4780-6   Nov 2006
PURPOSE: To elucidate the expression pattern of K15, K19, K14, and K12 in human and mouse ocular surface epithelium as putative markers of epithelial phenotype. METHODS: Immunohistochemical staining with specific antibodies for K15, K19, K14, and ...
Tomita, M., Shimmura, S., Tsubota, K. and Shimazaki, J.
Arch Ophthalmol   124(9) 1239-42   Sep 2006
OBJECTIVE: To report the incidence of dacryoadenitis as a symptom associated with Acanthamoeba keratitis. METHODS: We investigated all cases of Acanthamoeba keratitis (20 patients and 21 eyes) diagnosed and treated at Tokyo Dental College, Ichikaw...

Teaching Experience

 
 

Research Grants & Projects

 
ドラッグ・リポジショニングによるフックス角膜変性症治療薬の開発
MEXT,JSPS: Grant-in-Aid for Scientific Research
Project Year: Apr 2019 - Mar 2022    Investigator(s): 榛村 重人
フックス角膜内皮変性症の疾患特異的iPS細胞による病態解明
MEXT,JSPS: Grant-in-Aid for Scientific Research
Project Year: Apr 2016 - Mar 2019    Investigator(s): 榛村 重人

Social Contribution

 
Asian Cornea Society
[]  2009 - Today
日本炎症・再生医療学会
[]  2004 - Today
日本再生医療学会
[]  2003 - Today
American Academy of Ophthalmology
[]  2002 - Today
日本角膜学会
[]  1998 - Today