2010年12月
Norovirus-binding proteins recovered from activated sludge micro-organisms with an affinity to a noroviral capsid peptide
JOURNAL OF APPLIED MICROBIOLOGY
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- 巻
- 109
- 号
- 6
- 開始ページ
- 1923
- 終了ページ
- 1928
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1111/j.1365-2672.2010.04821.x
- 出版者・発行元
- WILEY-BLACKWELL PUBLISHING, INC
Aims: Transmission routes of noroviruses, leading aetiological agents of acute gastroenteritis, are rarely verified when outbreaks occur. Because the destination of norovirus particles being firmly captured by micro-organisms could be totally different from that of those particles moving freely, micro-organisms with natural affinity ligands such as virus-binding proteins would affect the fate of viruses in environment, if such microbial affinity ligands exist. The aim of this study is to identify norovirus-binding proteins (NoVBPs) that are presumably working as natural ligands for norovirus particles in water environments.
Methods and Results: NoVBPs were recovered from activated sludge microorganisms by an affinity chromatography technique in which a capsid peptide of norovirus genogroup II (GII) was immobilized. The recovered NoVBPs bind to norovirus-like particles (NoVLPs) of norovirus GII, and this adsorption was stronger than that to NoVLPs of norovirus genogroup I. The profile of two-dimensional electrophoresis of NoVBPs showed that the recovered NoVBPs included at least seven spots of protein. The determination of N-terminal amino acid sequences of these NoVBPs revealed that hydrophobic interactions could contribute to the adsorption between NoVBPs and norovirus particles.
Conclusions: NoVBPs conferring a high affinity to norovirus GII were successfully isolated from activated sludge micro-organisms.
Significance and Impact of the Study: NoVBPs could be natural viral ligands and play an important role in the NoV transmission.
Methods and Results: NoVBPs were recovered from activated sludge microorganisms by an affinity chromatography technique in which a capsid peptide of norovirus genogroup II (GII) was immobilized. The recovered NoVBPs bind to norovirus-like particles (NoVLPs) of norovirus GII, and this adsorption was stronger than that to NoVLPs of norovirus genogroup I. The profile of two-dimensional electrophoresis of NoVBPs showed that the recovered NoVBPs included at least seven spots of protein. The determination of N-terminal amino acid sequences of these NoVBPs revealed that hydrophobic interactions could contribute to the adsorption between NoVBPs and norovirus particles.
Conclusions: NoVBPs conferring a high affinity to norovirus GII were successfully isolated from activated sludge micro-organisms.
Significance and Impact of the Study: NoVBPs could be natural viral ligands and play an important role in the NoV transmission.
- リンク情報
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- DOI
- https://doi.org/10.1111/j.1365-2672.2010.04821.x
- CiNii Articles
- http://ci.nii.ac.jp/naid/80021502709
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20735509
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000284170300008&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1111/j.1365-2672.2010.04821.x
- ISSN : 1364-5072
- CiNii Articles ID : 80021502709
- PubMed ID : 20735509
- Web of Science ID : WOS:000284170300008