2002年12月
Regulation of taurine transport at the blood-brain barrier by tumor necrosis factor-alpha taurine and hypertonicity
JOURNAL OF NEUROCHEMISTRY
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- 巻
- 83
- 号
- 5
- 開始ページ
- 1188
- 終了ページ
- 1195
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1046/j.1471-4159.2002.01223.x
- 出版者・発行元
- BLACKWELL PUBLISHING LTD
Taurine is the abundant sulfur-containing beta-amino acid in brain where it exerts a neuroprotective effect. Although it is known that the blood-brain barrier (BBB) mediates taurine transport, the regulation of taurine transport have not been clarified yet. A conditionally immortalized rat brain capillary endothelial cells (TR-BBB13), an in vitro model of the BIBB, exhibited [H-3]taurine uptake, which was dependent on both Na+ and Cl-, and inhibited by beta-alanine. Taurine transporter (TAUT) mRNA was detected in TR-BBB13 cells, and TAUT protein was also expressed at 70 kDa. TR-BBB13 cells exposed to 20 ng/mL TNF-alpha and under hypertonic conditions showed a 1.7-fold and 3.2-fold increase in [H-3]taurine uptake, respectively. In contrast, lipopolysaccharide and diethyl maleate did not significantly affect taurine uptake. The taurine uptake was reduced by pre-treatment with excess taurine (50 mm). The mRNA level of the TAUT in TNF-alpha and following hypertonic treatment was greater than that in control cells, whereas that under excess taurine conditions was lower than in controls. Therefore, taurine transport activity at the BBB appears to be regulated at the transcriptional level by cell damage, osmolality and taurine in the brain.
- リンク情報
- ID情報
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- DOI : 10.1046/j.1471-4159.2002.01223.x
- ISSN : 0022-3042
- Web of Science ID : WOS:000179571400018