論文

査読有り
2014年7月

Characteristics and molecular phylogeny of Fasciola flukes from Bangladesh, determined based on spermatogenesis and nuclear and mitochondrial DNA analyses

PARASITOLOGY RESEARCH
  • Uday Kumar Mohanta
  • ,
  • Madoka Ichikawa-Seki
  • ,
  • Takuya Shoriki
  • ,
  • Ken Katakura
  • ,
  • Tadashi Itagaki

113
7
開始ページ
2493
終了ページ
2501
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00436-014-3898-5
出版者・発行元
SPRINGER

This study aimed to precisely discriminate Fasciola spp. based on DNA sequences of nuclear internal transcribed spacer 1 (ITS1) and mitochondrial nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 1 (nad1) gene. We collected 150 adult flukes from the bile ducts of cattle, buffaloes, sheep, and goats from six different regions of Bangladesh. Spermatogenic status was determined by analyzing stained seminal vesicles. The ITS1 types were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The nad1 haplotypes were identified based on PCR and direct sequencing and analyzed phylogenetically by comparing with nad1 haplotypes of Fasciola spp. from other Asian countries. Of the 127 aspermic flukes, 98 were identified as Fg type in ITS1, whereas 29 were identified as Fh/Fg type, indicating a combination of ITS1 sequences of Fasciola hepatica and Fasciola gigantica. All the 127 aspermic flukes showed Fsp-NDI-Bd11 in nad1 haplotype with nucleotide sequences identical to aspermic Fasciola sp. from Asian countries. Further, 20 spermic flukes were identified as F. gigantica based on their spermatogenic status and Fg type in ITS1. F. gigantica population was thought to be introduced into Bangladesh considerably earlier than the aspermic Fasciola sp. because 11 haplotypes with high haplotype diversity were detected from the F. gigantica population. However, three flukes from Bangladesh could not be precisely identified, because their spermatogenic status, ITS1 types, and nad1 haplotypes were ambiguous. Therefore, developing a robust method to distinguish aspermic Fasciola sp. from other Fasciola species is necessary in the future.

リンク情報
DOI
https://doi.org/10.1007/s00436-014-3898-5
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24781019
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000339965000010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00436-014-3898-5
  • ISSN : 0932-0113
  • eISSN : 1432-1955
  • PubMed ID : 24781019
  • Web of Science ID : WOS:000339965000010

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