2014年7月
Diversification of 13S globulins, allergenic seed storage proteins, of common buckwheat
FOOD CHEMISTRY
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- 巻
- 155
- 号
- 開始ページ
- 192
- 終了ページ
- 198
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.foodchem.2014.01.047
- 出版者・発行元
- ELSEVIER SCI LTD
The alpha polypeptide of the 13S globulin subunit of common buckwheat is the counterpart of the major allergenic beta polypeptide. Trypsin digestibility varies between variants of the alpha polypeptide with and without a tandem repeat insert. To evaluate the intra-species diversity of 13S globulin, the comprehensive screening of a genomic DNA library was performed, resulting in the isolation of 14 and 3 genes for Met-poor and Met-rich subunits, respectively. Although most tandem repeat units were 45 bp in length, the two-repeat gene Glb2B and all one-repeat genes contained an additional 3 bp. Secondary structure predictions and polyacrylamide gel electrophoresis demonstrated that the sense strand of Glb2B-CCG, the additional 3 bp-deletion clone of Glb2B, formed a more rigid secondary structure than that of the wild-type. Thus, the large intra-species variation of 13S globulin revealed in this study and its diversification might be attributable to the unique nature of the tandem repeat sequences. (C) 2014 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.foodchem.2014.01.047
- ISSN : 0308-8146
- eISSN : 1873-7072
- Web of Science ID : WOS:000333730000029