論文

査読有り 国際誌
2019年1月14日

Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes.

BMC research notes
  • Akira Ito
  • ,
  • Tomoki Aoyama
  • ,
  • Hirotaka Iijima
  • ,
  • Kohei Nishitani
  • ,
  • Junichi Tajino
  • ,
  • Hiroshi Kuroki

12
1
開始ページ
16
終了ページ
16
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13104-019-4058-x

OBJECTIVE: This study aimed to clarify the effects of periodic mild heat stimuli on extracellular matrix (ECM) synthesis of adult human chondrocytes in 3-dimensional pellet culture. RESULTS: Human articular chondrocytes were subjected to pellet culture at 37 °C for 3 days. Thereafter, the pellets were divided into three groups: 32 °C group which was cultured at 32 °C without heat stimuli, 32 °C + Heat group which was cultured at 32 °C and applied periodic heat stimuli, 37 °C group which was cultured at 37 °C. Heat stimuli were given by transferring the pellets into a CO2 incubator set at 41 °C for 20 min/day, 6 times/week. ECM synthesis ability was evaluated by analyzing the mRNA expressions. Additionally, the collagen and proteoglycan content in the pellet was quantified. DNA content was also measured for estimating the cell amount. We found that there were no significant differences in the mRNA expression of COL2A1, COL1A1, and ACAN between the 32 °C group and 32 °C + Heat group. However, the collagen content per cell and DNA content were significantly lower in the 32 °C + Heat group compared to other groups. Our results indicate that periodic mild heat stimuli may diminish ECM synthesis due to inhibition of collagen production and loss of cells.

リンク情報
DOI
https://doi.org/10.1186/s13104-019-4058-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30642366
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332905
ID情報
  • DOI : 10.1186/s13104-019-4058-x
  • PubMed ID : 30642366
  • PubMed Central 記事ID : PMC6332905

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