論文

査読有り
2014年2月

SP10 Infectivity Is Aborted after Bacteriophage SP10 Infection Induces nonA Transcription on the Prophage SP beta Region of the Bacillus subtilis Genome

JOURNAL OF BACTERIOLOGY
  • Tatsuya Yamamoto
  • ,
  • Nozomu Obana
  • ,
  • Lii Mien Yee
  • ,
  • Kei Asai
  • ,
  • Nobuhiko Nomura
  • ,
  • Kouji Nakamura

196
3
開始ページ
693
終了ページ
706
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/JB.01240-13
出版者・発行元
AMER SOC MICROBIOLOGY

Bacteria have developed various strategies for phage resistance. Infection with phage induces the transcription of part of the phage resistance gene, but the regulatory mechanisms of such transcription remain largely unknown. The phage resistance gene nonA is located on the SP beta prophage region of the Bacillus subtilis Marburg strain genome. The nonA transcript was detected at the late stage of SP10 infection but is undetectable in noninfected cells. The nonA transcript was detected after the induction of the sigma factor Orf199-Orf200 (sigma(Orf199-200)), when sigma factors encoded in the SP10 genome were expressed from a xylose-inducible plasmid. Thus, the SP10 sigma factor is an activator of a set of SP10 genes and nonA. The nonA gene encodes a 72-aminoacid protein with a transmembrane motif and has no significant homology with any protein in any database. NonA overexpression halted cell growth and reduced the efficiency of B. subtilis colony formation and respiration activity. In addition, SP10 virion protein synthesis was inhibited in the nonA(+) strain, and SP10 virion particles were scarce in it. These results indicate that NonA is a novel protein that can abort SP10 infection, and its transcription was regulated by SP10 sigma factor.

Web of Science ® 被引用回数 : 7

リンク情報
DOI
https://doi.org/10.1128/JB.01240-13
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000332625000019&DestApp=WOS_CPL

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