MISC

1998年4月

Defect of an early event of glucose metabolism in skeletal muscle of the male Otsuka Long-Evans Tokushima Fatty (OLETF) rat, a non-insulin-dependent diabetes mellitus (NIDDM) model

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • T Sato
  • ,
  • K Magata
  • ,
  • N Koga
  • ,
  • Y Mitsumoto

245
2
開始ページ
378
終了ページ
381
記述言語
英語
掲載種別
DOI
10.1006/bbrc.1998.8442
出版者・発行元
ACADEMIC PRESS INC

Otsuka Long-Evans Tokushima Fatty (OLETF) rats showed that the distribution of plasma membrane content of insulin-regulated glucose transporter in skeletal muscle was reminiscent of that in human non-insulin-dependent diabetes mellitus (NIDDM). To obtain more information on the cellular mechanisms of muscle insulin resistance, hexokinase activities were measured in the skeletal muscle of OLETF rats. The results showed that the activity of the type II enzyme in the diabetic rats was significantly decreased (P<0.05) compared with Long-Evans Tokushima Otsuka (LETO) control rats. No significant differences in the activity of the type I hexokinase were observed between these rats. Western blot analysis showed that the protein content of the type II in OLETF rats was also significantly lower than that in LETO rats (P<0.05). After insulin stimulation, the intramuscular content of glucose 6-phosphate, which regulates glycogen synthesis in skeletal muscle, was significantly decreased in OLETF rats (P<0.01). However, glycogen synthase activity in vitro and intramuscular lactate concentration in these rats did not show significant differences. These results suggest that the G6P content of the diabetic rats is decreased as a result of an impaired early event of glucose metabolism, indicating that the molecular defects of skeletal muscle in OLETF rats are similar to those in NIDDM patients. (C) 1988 Academic Press.

リンク情報
DOI
https://doi.org/10.1006/bbrc.1998.8442
CiNii Articles
http://ci.nii.ac.jp/naid/30017797804
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/9571158
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000073279500016&DestApp=WOS_CPL
ID情報
  • DOI : 10.1006/bbrc.1998.8442
  • ISSN : 0006-291X
  • CiNii Articles ID : 30017797804
  • PubMed ID : 9571158
  • Web of Science ID : WOS:000073279500016

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