2007年6月
Specific membrane recruitment of Uso1 protein, the essential endoplasmic reticulum-to-Golgi tethering factor in yeast vesicular transport
JOURNAL OF CELLULAR BIOCHEMISTRY
- ,
- ,
- 巻
- 101
- 号
- 3
- 開始ページ
- 686
- 終了ページ
- 694
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/jcb.21225
- 出版者・発行元
- WILEY-LISS
Uso1 is a yeast essential protein that functions to tether vesicles in the ER-to-Golgi transport. Its recruitment to the ER-derived vesicles has been demonstrated in in vitro membrane transport systems using semi-intact cells. Here we report that the binding of Uso1 to specific membranes can be detected through simple sucrose density block centrifugation. The purified Uso1 protein binds to slowly sedimenting membranes generated from rapidly sedimenting P10 membranes. These membranes were produced dependent on ATP hydrolysis, contained COPII vesicle components, but had neither of the coat subunits or ER proteins, which indicates that they were representative of the uncoated ER-derived COPII vesicles. The slowly sedimenting membranes of different origins were physically linked when they were mixed in the presence of Uso1. The C-terminal acidic region was not required in membrane binding. The presence of membranes to which Uso1 could bind in the yeast cell lysate was detected using the current method.
- リンク情報
-
- DOI
- https://doi.org/10.1002/jcb.21225
- CiNii Articles
- http://ci.nii.ac.jp/naid/80018393745
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17192843
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000246123400015&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1002/jcb.21225
- ISSN : 0730-2312
- CiNii Articles ID : 80018393745
- PubMed ID : 17192843
- Web of Science ID : WOS:000246123400015