論文

査読有り
2015年4月

PCTK1 Regulates Integrin-Dependent Spindle Orientation via Protein Kinase A Regulatory Subunit KAP0 and Myosin X

MOLECULAR AND CELLULAR BIOLOGY
  • Sayaka Iwano
  • ,
  • Ayaka Satou
  • ,
  • Shigeru Matsumura
  • ,
  • Naoyuki Sugiyama
  • ,
  • Yasushi Ishihama
  • ,
  • Fumiko Toyoshima

35
7
開始ページ
1197
終了ページ
1208
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/MCB.01017-14
出版者・発行元
AMER SOC MICROBIOLOGY

Integrin-dependent cell-extracellular matrix (ECM) adhesion is a determinant of spindle orientation. However, the signaling pathways that couple integrins to spindle orientation remain elusive. Here, we show that PCTAIRE-1 kinase (PCTK1), a member of the cyclin-dependent kinases (CDKs) whose function is poorly characterized, plays an essential role in this process. PCTK1 regulates spindle orientation in a kinase-dependent manner. Phosphoproteomic analysis together with an RNA interference screen revealed that PCTK1 regulates spindle orientation through phosphorylation of Ser83 on KAP0, a regulatory subunit of protein kinase A (PKA). This phosphorylation is dispensable for KAP0 dimerization and for PKA binding but is necessary for its interaction with myosin X, a regulator of spindle orientation. KAP0 binds to the FERM domain of myosin X and enhances the association of myosin X-FERM with beta 1 integrin. This interaction between myosin X-FERM and beta 1 integrin appeared to be crucial for spindle orientation control. We propose that PCTK1-KAP0-myosin X-beta 1 integrin is a functional module providing a link between ECM and the actin cytoskeleton in the ECM-dependent control of spindle orientation.

リンク情報
DOI
https://doi.org/10.1128/MCB.01017-14
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25605337
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355539
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000351739200010&DestApp=WOS_CPL
URL
http://europepmc.org/articles/PMC4355539
URL
http://orcid.org/0000-0001-7633-8495
ID情報
  • DOI : 10.1128/MCB.01017-14
  • ISSN : 0270-7306
  • eISSN : 1098-5549
  • ORCIDのPut Code : 58378583
  • PubMed ID : 25605337
  • PubMed Central 記事ID : PMC4355539
  • Web of Science ID : WOS:000351739200010

エクスポート
BibTeX RIS