Tsuyoshi Hirota

Profile Information

Affiliation: Designated Associate Professor, Institute of Transformative Bio-Molecules, Nagoya University

Degree: Master (Science)(The University of Tokyo)
Ph.D (Science)(The University of Tokyo)

Researcher number: 50372412
ORCID ID: https://orcid.org/0000-0003-4876-3608
J-GLOBAL ID: 200901030055713604
researchmap Member ID: 1000316039

External link: http://www.itbm.nagoya-u.ac.jp/en/kay-hirota_group/

Research Areas

Life sciences / Functional biochemistry /

Research History

May, 2014 - Present

Designated Associate Professor, Institute of Transformative Bio-Molecules, Nagoya University
Oct, 2014 - Mar, 2018

PRESTO Researcher (concurrent), Japan Science and Technology Agency
Jul, 2013 - Apr, 2014

Research Associate, University of Southern California
Jul, 2012 - Jul, 2013

Associate Research Specialist, UC San Diego
Oct, 2008 - Jun, 2012

Assistant Research Specialist, UC San Diego
Sep, 2007 - Sep, 2008

Postdoctoral Scholar, UC San Diego
Apr, 2007 - Sep, 2007

Research Associate, The Scripps Research Institute
Apr, 2003 - Mar, 2007

Project Assistant Professor, Graduate School of Science, The University of Tokyo
Apr, 2000 - Mar, 2003

Research Fellow (DC1), JSPS

Education

Apr, 1998 - Mar, 2003

Department of Biophysics and Biochemistry, Grasuate School of Science, The University of Tokyo
Apr, 1996 - Mar, 1998

Department of Biophysics and Biochemistry, School of Science, The University of Tokyo

Papers

Development of a novel PET ligand, [11C]GO289 targeting CK2 expressed in the brain.

Aya Ogata, Takashi Yamada, Saori Hattori, Hiroshi Ikenuma, Junichiro Abe, Mari Tada, Masanori Ichise, Masaaki Suzuki, Kengo Ito, Takashi Kato …

Bioorganic & medicinal chemistry letters, 90 129327-129327, Jun 15, 2023 Peer-reviewed
Analysis of the phosphoproteome of CK2α(-/-)/Δα' C2C12 myoblasts compared to the wild-type cells.

Christian Borgo, Luca Cesaro, Tsuyoshi Hirota, Keiko Kuwata, Claudio D'Amore, Thomas Ruppert, Renata Blatnik, Mauro Salvi, Lorenzo A Pinna

Open biology, 13(2) 220220-220220, Feb, 2023 Peer-reviewed
CRY2 isoform selectivity of a circadian clock modulator with antiglioblastoma efficacy.

Simon Miller, Manish Kesherwani, Priscilla Chan, Yoshiko Nagai, Moeri Yagi, Jamie Cope, Florence Tama, Steve A Kay, Tsuyoshi Hirota

Proceedings of the National Academy of Sciences of the United States of America, 119(40) e2203936119, Oct 4, 2022 Peer-reviewed
A methylbenzimidazole derivative regulates mammalian circadian rhythms by targeting Cryptochrome proteins

Moeri Yagi, Simon Miller, Yoshiko Nagai, Shinsuke Inuki, Ayato Sato, Tsuyoshi Hirota

F1000Research, 11 1016-1016, Sep 7, 2022 Peer-reviewed
Phosphorylation of RNA Polymerase II by CDKC;2 Maintains the Arabidopsis Circadian Clock Period.

Takahiro N Uehara, Takashi Nonoyama, Kyomi Taki, Keiko Kuwata, Ayato Sato, Kazuhiro J Fujimoto, Tsuyoshi Hirota, Hiromi Matsuo, Akari E Maeda, Azusa Ono …

Plant & cell physiology, 63(4) 450-462, Jan 27, 2022 Peer-reviewed
Structural and Chemical Biology Approaches Reveal Isoform-Selective Mechanisms of Ligand Interactions in Mammalian Cryptochromes

Miller, S., Hirota, T.

Frontiers in Physiology, 13 837280-837280, 2022 Peer-reviewed
Cell-Based Phenotypic Screens to Discover Circadian Clock-Modulating Compounds.

Megumi Hatori, Tsuyoshi Hirota

Methods in molecular biology (Clifton, N.J.), 2482 95-104, 2022 Peer-reviewed
Modulation of circadian clock by crude drug extracts used in Japanese Kampo medicine.

Manhui Zhang, Kohei Kobayashi, Haruki Atsumi, Yuma Katada, Yusuke Nakane, Junfeng Chen, Ryo Nagano, Naoya Kadofusa, Taeko Nishiwaki-Ohkawa, Naohiro Kon …

Scientific reports, 11(1) 21038-21038, Oct 26, 2021 Peer-reviewed
Effects of Cryptochrome-modulating compounds on circadian behavioral rhythms in zebrafish.

Mui Iida, Yusuke Nakane, Takashi Yoshimura, Tsuyoshi Hirota

Journal of biochemistry, 171(5) 501-507, Sep 16, 2021 Peer-reviewed
Structural differences in the FAD-binding pockets and lid loops of mammalian CRY1 and CRY2 for isoform-selective regulation

Simon Miller, Ashutosh Srivastava, Yoshiko Nagai, Yoshiki Aikawa, Florence Tama, Tsuyoshi Hirota

Proceedings of the National Academy of Sciences, 118(26), Jun 29, 2021 Peer-reviewed
Reversible modulation of circadian time with chronophotopharmacology.

Kolarski D, Miró-Vinyals C, Sugiyama A, Srivastava A, Ono D, Nagai Y, Iida M, Itami K, Tama F, Szymanski W …

Nature communications, 12(1) 3164-3164, May, 2021 Peer-reviewed
Comparing the efficacy and selectivity of Ck2 inhibitors. A phosphoproteomics approach

Christian Borgo, Luca Cesaro, Tsuyoshi Hirota, Keiko Kuwata, Claudio D’Amore, Thomas Ruppert, Renata Blatnik, Mauro Salvi, Lorenzo A. Pinna

European Journal of Medicinal Chemistry, 214 113217-113217, Mar, 2021 Peer-reviewed
Photopharmacological Manipulation of Mammalian CRY1 for Regulation of the Circadian Clock

Dušan Kolarski, Simon Miller, Tsuyoshi Oshima, Yoshiko Nagai, Yugo Aoki, Piermichele Kobauri, Ashutosh Srivastava, Akiko Sugiyama, Kazuma Amaike, Ayato Sato …

Journal of the American Chemical Society, 143(4) 2078-2087, Jan 19, 2021 Peer-reviewed
Reductive stability evaluation of 6-azopurine photoswitches for the regulation of CKIα activity and circadian rhythms

Dušan Kolarski, Akiko Sugiyama, Theo Rodat, Albert Schulte, Christian Peifer, Kenichiro Itami, Tsuyoshi Hirota, Ben L. Feringa, Wiktor Szymanski

Organic & Biomolecular Chemistry, 19(10) 2312-2321, 2021 Peer-reviewed
A N-terminally deleted form of the CK2α’ catalytic subunit is sufficient to support cell viability

Christian Borgo, Claudio D’Amore, Luca Cesaro, Kenichiro Itami, Tsuyoshi Hirota, Mauro Salvi, Lorenzo A. Pinna

Biochemical and Biophysical Research Communications, 531(3) 409-415, Oct, 2020 Peer-reviewed
An Isoform-Selective Modulator of Cryptochrome 1 Regulates Circadian Rhythms in Mammals

Simon Miller, Yoshiki Aikawa, Akiko Sugiyama, Yoshiko Nagai, Aya Hara, Tsuyoshi Oshima, Kazuma Amaike, Steve A. Kay, Kenichiro Itami, Tsuyoshi Hirota

Cell Chemical Biology, 27(9) 1192-1198, Jun, 2020 Peer-reviewed
“Janus” efficacy of CX-5011: CK2 inhibition and methuosis induction by independent mechanisms

D'Amore, C., Moro, E., Borgo, C., Itami, K., Hirota, T., Pinna, L.A., Salvi, M.

Biochimica et Biophysica Acta - Molecular Cell Research, 1867(11) 118807-118807, 2020 Peer-reviewed
Small Molecules Modulating Mammalian Biological Clocks: Exciting New Opportunities for Synthetic Chemistry

Amaike, K., Oshima, T., Skoulding, N.S., Toyama, Y., Hirota, T., Itami, K.

Chem, 6(9), 2020 Peer-reviewed
Isoform-selective regulation of mammalian cryptochromes

Miller, S., Son, Y.L., Aikawa, Y., Makino, E., Nagai, Y., Srivastava, A., Oshima, T., Sugiyama, A., Hara, A., Abe, K. …

Nature Chemical Biology, 16(6) 676-685, 2020 Peer-reviewed
Pharmacological Interventions to Circadian Clocks and Their Molecular Bases

Miller, S., Hirota, T.

Journal of Molecular Biology, 432(12) 3498-3514, 2020 Peer-reviewed

Misc.

細胞外pHのアルカリ性化はDec1遺伝子の誘導を介して概日時計の位相をリセットする

金尚宏, 広田毅, 河本健, 加藤幸夫, 坪田匡史, 深田吉孝

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 81回・31回 4T16-4, Nov, 2008
ニワトリ松果体におけるコレステロール合成系酵素群の遺伝子発現解析

飯塚倫子, 倉林伸博, 羽鳥恵, 広田毅, 原口省吾, 筒井和義, 深田吉孝

日本比較生理生化学会大会予稿集, 30th 23, 2008
Sequential phosphorylation of mouse cryptochrome2

Nobuhiro Kurabayashi, Tsuyoshi Hirota, Yoko Harada, Mihiko Sakai, Yoshitaka Fukada

ZOOLOGICAL SCIENCE, 23(12) 1194-1194, Dec, 2006
【ここまで分かった生物時計の分子ネットワークリン酸化による時計タンパク質の制御機構から睡眠・肥満への関与まで】時計タンパク質の多段階リン酸化

広田毅, 深田吉孝

実験医学, 24(4) 452-459, Mar, 2006
分子時計の生化学グルコース投与によるrat-1細胞の時計遺伝子発現における概日リズムの誘導

広田毅, 岡野俊行, 小亀浩市, 池島裕子, 谷, 宮田敏行, 深田吉孝

生化学, 74(8) 643-643, Aug, 2002
ニワトリ松果体の概日時計構成分子cBMAL2

岡野俊行, 山本和幸, 岡野恵子, 広田毅, 佐々木桃子, 高中陽子, 深田吉孝

日本時間生物学会会誌: Journal of Chronobiology, 6(2) 87-87, Oct 1, 2000
ブレフェルジンAを用いたニワトリ松果体細胞のメラトニン分泌機構の解析

広田毅, 鍵和田聡, 笠原和起, 岡野俊行, 村田昌之, 深田吉孝

生化学, 72(8) 1016-1016, Aug, 2000
IDENTIFICATION OF CHICKEN PINEAL CLOCK GENES(Physiology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan :

Okano T., Yamamoto K., Okano K., Hirota T., Sasaki M., Takanaka Y., Fukada Y.

Zoological science, 17 92-92, 2000
松果体とサーカディアンリズム

深田吉孝, 広田毅

Clinical Neuroscience特集「サーカディアンリズムのしくみと働き」, 18(10) 1147-1149, 2000
Inhibition of metatonin synthesis in chick pineal cells by pulse treatment with brefeldin A

Hirota T., Kagiwada S., Kasahara T., Okano T., Fukada Y., Murata M.

Seibutsu Butsuri, 39 S48, 1999
時計細胞における概日時計の光リセット機構と時刻可視化の試み

笠原和起, 広田毅, 佐々木桃子, 岡野俊行, 深田吉孝

バイオイメージング, 7(3) 163-164, Oct 1, 1998
Effects of brefeldin A on melatonin production in chick pineal cells

Hirota T., Kagiwada S., Kasahara T., Okano T., Fukada Y., Murata M.

Biophysics, 38(2) S47, Sep 7, 1998

Research Projects

革新的な機能調節化合物の創製による概日時計システムの統合的な理解と制御

科学研究費助成事業, 基盤研究(A), 日本学術振興会, Apr, 2021 - Mar, 2024

廣田毅
ケージド時計調節化合物を用いた概日リズムの光制御と細胞間相互作用の解析

科学研究費助成事業, 挑戦的研究(萌芽), 日本学術振興会, Jul, 2020 - Mar, 2023

廣田毅
Mechanistic study on circadian clock system regulated by heme/CO crosstalk

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
Isoform selectivity of circadian clock proteins CRY1 and CRY2 analyzed by new chemical tools

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Hirota Tsuyoshi
Development and characterization of small molecules for analysis of circadian rhythm amplitude

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Japan Society for the Promotion of Science, Jun, 2018 - Mar, 2020

Hirota Tsuyoshi
Development and characterization of small molecules that regulate clock protein function for analysis of the circadian clock mechanism

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (A), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

Hirota Tsuyoshi, ITAMI Kenichiro, Jeffery Bode, SATO Ayato, KUWATA Keiko
概日時計タンパク質CRYの機能を調節する低分子化合物

科学研究費助成事業, 研究活動スタート支援, 日本学術振興会, Aug, 2014 - Mar, 2015

廣田毅
Molecular, cellular and in vivo analyses of photoresponses and circadian rhythms in animals

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), Japan Society for the Promotion of Science, 2002 - 2006

FUKADA Yoshitaka, KOJIMA Daisuke, HIROTA Tsuyoshi
哺乳類の末梢組織における概日時計のグルコース応答

科学研究費助成事業, 若手研究(B), 日本学術振興会, 2004 - 2005

広田毅

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