論文

国際誌
2021年2月1日

Identification of fucosylated haptoglobin-producing cells in pancreatic cancer tissue and its molecular mechanism.

Glycoconjugate journal
  • Nami Ito
  • Momoko Yamada
  • Koichi Morishita
  • Satoshi Nojima
  • Kei Motooka
  • Natsumi Sakata
  • Tatsuya Asuka
  • Ryoji Otsu
  • Shinji Takamatsu
  • Yoshihiro Kamada
  • Soichiro Mori
  • Hirofumi Akita
  • Hidetoshi Eguchi
  • Eiichi Morii
  • Eiji Miyoshi
  • 全て表示

38
1
開始ページ
45
終了ページ
54
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10719-020-09970-8

Fucosylated haptoglobin is a well-established glyco-biomarker of pancreatic cancer. We recently established a novel anti-glycan antibody (10-7G mAb) that specifically recognizes fucosylated haptoglobins, including prohaptoglobin (proHpt). Serum concentrations of the 10-7G value, as measured by ELISA, were increased in patients with pancreatic cancer relative to the healthy controls. However, it is currently unknown which specific tissue or cell type produces fucosylated haptoglobins or proHpt. In the present study, we performed immunohistochemical (IHC) and ELISA analyses of pancreatic cancer tissue samples using 10-7G mAb. Among 21 pancreatic tissue sections, only 1 showed direct staining of pancreatic cells with the 10-7G mAb. However, 12 of the 21 sections stained positively for immune cells. Although there was no significant difference in the 10-7G expression between the positive and negative staining IHC groups, the median value of serum 10-7G was slightly higher in IHC-positive cases. Among many assayed leukemic cell lines, differentiated THP-1 cells (a human acute monocytic leukemia cell line) were found to have the highest levels of proHpt, per Western blot using 10-7G mAb. Interestingly, production of proHpt in vitro was dramatically increased under either hypoxic conditions or after IL-6 treatment. These results suggest that immune cells, including macrophages, in the pancreatic tissue microenvironment produce fucosylated haptoglobin and proHpt. Thus, fucosylated haptoglobins can be detected by the 10-7G mAb and may be a promising biomarker for pancreatic cancer.

リンク情報
DOI
https://doi.org/10.1007/s10719-020-09970-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33523362
ID情報
  • DOI : 10.1007/s10719-020-09970-8
  • PubMed ID : 33523362

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