広津建 (Ken Hirotu) - マイポータル - researchmap

基本情報

所属: 旧所属大阪市立大学大学院理学研究科物質分子系専攻（分子相関化学）教授

J-GLOBAL ID: 200901041421167920
researchmap会員ID: 1000322560

研究分野

2

学歴

4

MISC

30

Substrate recognition mechanism of thermophilic dual-substrate enzyme

H Ura, T Nakai, S Kawaguchi, Miyahara, I, K Hirotsu, S Kuramitsu

JOURNAL OF BIOCHEMISTRY 130(1) 89-98 2001年7月
Structures of Escherichia coli branched-chain amino acid aminotransferase and its complexes with 4-methylvalerate and 2-methylleucine: Induced fit and substrate recognition of the enzyme

K Okada, K Hirotsu, H Hayashi, H Kagamiyama

BIOCHEMISTRY 40(25) 7453-7463 2001年6月
Structures of Escherichia coli histidinol-phosphate aminotransferase and its complexes with histidinol-phosphate and N-(5 '-phosphopyridoxyl)-L-glutamate: Double substrate recognition of the enzyme

K Haruyama, T Nakai, Miyahara, I, K Hirotsu, H Mizuguchi, H Hayashi, H Kagamiyama

BIOCHEMISTRY 40(15) 4633-4644 2001年4月
Porcine kidney D-amino acid oxidase: the three-dimensional structure and its catalytic mechanism based on the enzyme-substrate complex model

R Miura, C Setoyama, Y Nishina, K Shiga, Miyahara, I, H Mizutani, K Hirotsu

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 12(1-6) 43-52 2001年2月
Strain is more important than electrostatic interaction in controlling the pK(a) of the catalytic group in aspartate aminotransferase

H Mizuguchi, H Hayashi, K Okada, Miyahara, I, K Hirotsu, H Kagamiyama

BIOCHEMISTRY 40(2) 353-360 2001年1月
Inclusion behavior of bis(hydroxydiphenylmethyl)cyclohexane as a potential host compound

H Mizutani, J Ohta, Miyahara, I, K Hirotsu, K Tanaka, F Toda

SUPRAMOLECULAR CHEMISTRY 13(1) 53-60 2001年
Preferential enrichment and crystal structure

R Tamura, H Takahashi, K Hirotsu, Y Nakajima, T Ushio

MOLECULAR CRYSTALS AND LIQUID CRYSTALS 356 185-194 2001年
Stereochemistry of the transamination reaction catalyzed by aminodeoxychorismate lyase from Escherichia coli: Close relationship between fold type and stereochemistry

KH Jhee, T Yoshimura, EW Miles, S Takeda, Miyahara, I, K Hirotsu, K Soda, Y Kawata, N Esaki

JOURNAL OF BIOCHEMISTRY 128(4) 679-686 2000年10月
Three-dimensional structure of 4-amino-4-deoxychorismate lyase from Escherichia coli

T Nakai, H Mizutani, Miyahara, I, K Hirotsu, S Takeda, KH Jhee, T Yoshimura, N Esaki

JOURNAL OF BIOCHEMISTRY 128(1) 29-38 2000年7月
Three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase. Optimization of the oxidative half-reaction through alignment of the product with reduced flavin

H Mizutani, Miyahara, I, K Hirotsu, Y Nishina, K Shiga, C Setoyama, R Miura

JOURNAL OF BIOCHEMISTRY 128(1) 73-81 2000年7月
Free Energy Requirement for Domain Movement of an Enzyme

J. Biol. Chem. 275(25) 18939-18945 2000年
An new host 2,3,6,7,10,11-hexahydroxy triphenylene which forms chiral inclusion lattice: X-ray structural study of the chiral crystalline lattice

J. Phys. Org. Chem. 13 39-45 2000年
The crystal structure of pyroglutamyl peptidase I from Bacillus amyloliquefaciens reveals a new structure for a cysteine protease

Y Odagaki, A Hayashi, K Okada, K Hirotsu, T Kabashima, K Ito, T Yoshimoto, D Tsuru, M Sato, J Clardy

STRUCTURE 7(4) 399-411 1999年4月
Structure of Thermus thermophilus HB8 aspartate aminotransferase and its complex with maleate

T Nakai, K Okada, S Akutsu, Miyahara, I, S Kawaguchi, R Kato, S Kuramitsu, K Hirotsu

BIOCHEMISTRY 38(8) 2413-2424 1999年2月
N-tert-Butoxycarbonyl-N,N'-ethylene-bridged-(S)-tyrosyl-(S)-thyrosine methyl ester

Acta Crystallogr. C55 1023 1999年
Single crystal to single crystal enantioselective [2+2] photodimerization of cyclohex-2-enone as an inclusion complex with (-)-1,4-bis[3-(o-chlorophenyl)-3-hydroxy-3-phenylprop-1-ynyl]benzene

CrystEngComm 1993 3 1999年
Enantioselective Single-Crystal-to-Single-Crystal Photodimerization of Coumarin and Thiocoumarin in Inclusion Complexes with Chiral Host Compounds

Angew. Chem. Int. Ed. 38(23) 3523-3525 1999年
Extremely long C-C bond in (-)-trans-1,2-di-tert-butyl-1,2-diphenyl- and 1,1-di-tert-butyl-2,2-diphenyl- 3,8-dichlorocyclobuta[b]naphthalenes

J. Org. Chem. 64(9) 3102-3105 1999年
The active site of Paracoccus denitrificans aromatic amino acid aminotransferase has contrary properties: Flexibility and rigidity

A Okamoto, S Ishii, K Hirotsu, H Kagamiyama

BIOCHEMISTRY 38(4) 1176-1184 1999年1月
Crystallization and Preliminary X-ray Characterization of Aspartate Aminotransferase from an Extreme Thermophile, Thermus thermophilus HB8

Acta Crystallogr. D54(5) 1032 1998年

もっとみる

書籍等出版物

4

Refined Reaction Mechanism of Aspartate Aminotransferase

Birkhauser Verlag Basel/Switzerland 2000年
Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent ProteiStructure, Induced Fit and Substrate Recognition of E. coli Branched-Chain Amino Acid in Aminotransferasens

Birkhauser Verlag Basel/Switzerland 2000年
Flavins and Flavoproteins: The reaction scheme for D-amino acid oxidase: Substrate/product alignment for optimization of reductive and oxidative half-reactions

Agency for Scientific Publ., Berlin 1999年
Flavins and Flavoproteins: Role of the hydrogen-bonding network associated with isoalloxazine in the catalysis of D-amino acid oxidase

Agency for Scientific Publ., Berlin 1999年

所属学協会

3

共同研究・競争的資金等の研究課題

5

補酵素依存型タンパク質の立体構造と機能

科学研究費補助金 2000年
タンパク質のX線結晶構造解析

その他の研究制度
生体高分子の構造と機能

その他の研究制度
X-ray crystallographic determination of proteins

The Other Research Programs
Structure and Function of Biological Macromolecule

The Other Research Programs