論文

査読有り
2007年10月

Comparison of usefulness between Variable Numbers of Tandem Repeats (VNTR) analysis and Restriction Fragment Length Polymorphism (RFLP) in the genotyping of Mycobacterium avium

Kekkaku
  • Yuko Kazumi
  • ,
  • Tadashi Udagawa
  • ,
  • Shinji Maeda
  • ,
  • Yoshirou Murase
  • ,
  • Isamu Sugawara
  • ,
  • Masao Okumura
  • ,
  • Yuka Azuma
  • ,
  • Mieko Goto
  • ,
  • Noriko Tsunematsu

82
10
開始ページ
741
終了ページ
748
記述言語
日本語
掲載種別
研究論文(学術雑誌)
DOI
10.11400/kekkaku1923.82.741

[Objectives] Comparison of usefulness of IS 1245 RFLP and VNTR in M. avium genotyping. [Materials and methods] Thirty-six cases (55 strains) from sputum and BALF and twelve cases (29 strains) isolated from blood of HIV-infected patients were used. VNTR and RFLP using IS1245 were performed. [Result] Multiple samples were taken from 16 patients and 52 clinical isolates were used for VNTR and RFLP for comparison. (I) VNTR and RFLP results were identical in 12 out of 16 cases whose samples were collected several times. (2) Eight isolates were obtained from one patient. In this eight isolates, there were the cases of M. avium polyclonal infection and of mixed infection with M. intracellulare. VNTR patterns were two types and RFLP were 5 kinds of different in this case. (3) VNTR patterns of six isolates from one HIV-infected patient were identical, but there were three variations in RFLP patterns. There were three cases of mixed infections with M. tuberculosis or M. intracellulare, and six strains polyclonal infection of M. avium (7.1%) in 84 isolates. These 6 clinical isolates were derived from sputum or BALF (5 strains) and HIVinfected blood (one strain). VNTR patterns were similar in four pairs (9 strains) who did not contact closely, but they were distinguished clearly by RFLP. Seventeen strains had three or less IS1245-related bands in RFLP analyses of 89 strains. [Discussion] As there is a possibility of polyclonal infection with M. avium and mixed infection with other species, the single clonal infection should be confirmed first by VNTR. When single colony was obtained, VNTR and RFLP were performed for genotyping of M. avium. Furthermore, strains with less bands by RFLP should be carefully judged in terms of both VNTR and RFLP. It is recommended that the specimens should be collected from each patient several times.

リンク情報
DOI
https://doi.org/10.11400/kekkaku1923.82.741
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18018598
ID情報
  • DOI : 10.11400/kekkaku1923.82.741
  • ISSN : 0022-9776
  • PubMed ID : 18018598
  • SCOPUS ID : 38449119688

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