MISC

2001年1月5日

Comb-type cationic copolymer expedites DNA strand exchange while stabilizing DNA duplex

Chemistry - A European Journal
  • Won Jong Kim
  • ,
  • Tsutomu Ishihara
  • ,
  • Toshihiro Akaike
  • ,
  • Atsushi Maruyama

7
1
開始ページ
176
終了ページ
180
記述言語
英語
掲載種別
DOI
10.1002/1521-3765(20010105)7:1<176::AID-CHEM176>3.0.CO;2-M

The accelerating effect of cationic substances on the DNA strand exchange reaction between a 20 bp DNA duplex and its complementary single strand was studied. A polycationic comb-type copolymer, that consists of a poly(L-lysine) backbone and a dextran graft chain (PLL-g-Dex) and known to stabilize triplex DNA, expedites the strand exchange reaction under physiological relevant conditions. Electrostatically a small excess of the copolymer let to a 300 - 1500-fold increase in the DNA strand exchange while large excess of spermine or cetyltrimethylammonium bromide, a cationic detergent known to promote markedly hybridization of complementary DNA strands, shows only a slight effect. The efficacy of the copolymer was not affected by a 10mM Mg2+ concentration. Notably the copolymer promotes the strand exchange reaction while it stabilizes double-stranded DNA. The stabilization of strand exchange intermediates consisting of the parent duplex and the single strand by the copolymer is believed to be responsible for the observed acceleration behavior.

リンク情報
DOI
https://doi.org/10.1002/1521-3765(20010105)7:1<176::AID-CHEM176>3.0.CO;2-M
CiNii Articles
http://ci.nii.ac.jp/naid/80012153477
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/11205009
ID情報
  • DOI : 10.1002/1521-3765(20010105)7:1<176::AID-CHEM176>3.0.CO;2-M
  • ISSN : 0947-6539
  • CiNii Articles ID : 80012153477
  • PubMed ID : 11205009
  • SCOPUS ID : 0035808296

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