論文

査読有り
2017年9月

Liver Graft Preservation Using Perfluorocarbon Improves the Outcomes of Simulated Donation After Cardiac Death Liver Transplantation in Rats

LIVER TRANSPLANTATION
  • Shinya Okumura
  • Tadahiro Uemura
  • Xiangdong Zhao
  • Yuki Masano
  • Tatsuaki Tsuruyama
  • Yasuhiro Fujimoto
  • Taku Iida
  • Shintaro Yagi
  • Dmitri Bezinover
  • Bruce Spiess
  • Toshimi Kaido
  • Shinji Uemoto
  • 全て表示

23
9
開始ページ
1171
終了ページ
1185
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/lt.24806
出版者・発行元
WILEY

The outcomes of liver transplantation (LT) from donation after cardiac death (DCD) donors remain poor due to severe warm ischemia injury. Perfluorocarbon (PFC) is a novel compound with high oxygen carrying capacity. In the present study, a rat model simulating DCD LT was used, and the impact of improved graft oxygenation provided by PFC addition on liver ischemia/reperfusion injury (IRI) and survival after DCD LT was investigated. Orthotopic liver transplants were performed in male Lewis rats, using DCD liver grafts preserved with cold University of Wisconsin (UW) solution in the control group and preserved with cold oxygenated UW solution with addition of 20% PFC in the PFC group. For experiment I, in a 30-minute donor warm ischemia model, postoperative graft injury was analyzed at 3 and 6 hours after transplantation. For experiment II, in a 50-minute donor warm ischemia model, the postoperative survival was assessed. For experiment I, the levels of serum aspartate aminotransferase, alanine aminotransferase, hyaluronic acid, malondialdehyde, and several inflammatory cytokines were significantly lower in the PFC group. The hepatic expression levels of tumor necrosis factor alpha and interleukin 6 were significantly lower, and the expression level of heme oxygenase 1 was significantly higher in the PFC group. Histological analysis showed significantly less necrosis and apoptosis in the PFC group. Sinusoidal endothelial cells and microvilli of the bile canaliculi were well preserved in the PFC group. For experiment II, the postoperative survival rate was significantly improved in the PFC group. In conclusion, graft preservation with PFC attenuated liver IRI and improved postoperative survival. This graft preservation protocol might be a new therapeutic option to improve the outcomes of DCD LT.

リンク情報
DOI
https://doi.org/10.1002/lt.24806
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28650112
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000409898100010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/lt.24806
  • ISSN : 1527-6465
  • eISSN : 1527-6473
  • PubMed ID : 28650112
  • Web of Science ID : WOS:000409898100010

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