2013年
3D super-resolution fluorescence microscopy using cylindrical vector beams
Progress in Electromagnetics Research Letters
- ,
- 巻
- 43
- 号
- 開始ページ
- 73
- 終了ページ
- 81
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2528/PIERL13080205
- 出版者・発行元
- Electromagnetics Academy
We propose a method to obtain nano-scale 3D super- resolution in STED °uorescence microscopy. A double-ring-shaped cylindrical vector vortex beam, with an appropriate vortex angle and a proper truncation parameter of the beam, is used to generate a 3D dark spot as the erase spot. A single-ring-shaped radially polarized beam is used as a pump beam, which can generate a sharper 3D bright spot. The volume of the generated 3D dark spot is small and the uniformity of the light wall surrounding the spot is quite high. Consequently, the 3D super-resolution ability of a STED microscope is improved and nano-scale three-dimensional resolutions are obtained.
- ID情報
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- DOI : 10.2528/PIERL13080205
- ISSN : 1937-6480
- SCOPUS ID : 84885028263