論文

査読有り
2013年

3D super-resolution fluorescence microscopy using cylindrical vector beams

Progress in Electromagnetics Research Letters
  • Taikei Suyama
  • ,
  • Yaoju Zhang

43
開始ページ
73
終了ページ
81
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2528/PIERL13080205
出版者・発行元
Electromagnetics Academy

We propose a method to obtain nano-scale 3D super- resolution in STED °uorescence microscopy. A double-ring-shaped cylindrical vector vortex beam, with an appropriate vortex angle and a proper truncation parameter of the beam, is used to generate a 3D dark spot as the erase spot. A single-ring-shaped radially polarized beam is used as a pump beam, which can generate a sharper 3D bright spot. The volume of the generated 3D dark spot is small and the uniformity of the light wall surrounding the spot is quite high. Consequently, the 3D super-resolution ability of a STED microscope is improved and nano-scale three-dimensional resolutions are obtained.

リンク情報
DOI
https://doi.org/10.2528/PIERL13080205
ID情報
  • DOI : 10.2528/PIERL13080205
  • ISSN : 1937-6480
  • SCOPUS ID : 84885028263

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