2013年6月
ght2 (+) is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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- 巻
- 97
- 号
- 11
- 開始ページ
- 4957
- 終了ページ
- 4964
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00253-012-4637-4
- 出版者・発行元
- SPRINGER
Schizosaccharomyces pombe has eight hexose transporter genes, ght1 (+) to ght8 (+). Here we report that ght2 (+), which is highly expressed in the presence of glucose, is essential for UDP-galactose synthesis from extracellular galactose when cells grow on glucose. The galactosylation defect of a uge1 Delta mutant defective in synthesis of UDP-galactose from glucose was suppressed in galactose-containing medium, but disruption of ght2 (+) in the uge1 Delta mutant reversed suppression of the galactosylation defect. Expression of Saccharomyces cerevisiae GAL2 in uge1 Delta ght2 Delta cells suppressed the defective galactosylation phenotype in galactose-containing medium. These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose.
- リンク情報
- ID情報
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- DOI : 10.1007/s00253-012-4637-4
- ISSN : 0175-7598
- eISSN : 1432-0614
- Web of Science ID : WOS:000319136300025