2009年12月
Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation
MICROBIOLOGY-SGM
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- 巻
- 155
- 号
- 12
- 開始ページ
- 3816
- 終了ページ
- 3826
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1099/mic.0.034389-0
- 出版者・発行元
- MICROBIOLOGY SOC
Autophagy is triggered when organisms sense radical environmental changes, including nutritional starvation. During autophagy, cytoplasmic components, including organelles, are enclosed within autophagosomes and are degraded upon lysosome-vacuole fusion. In this study, we show that processing of GFP-tagged Atg8 can serve as a marker for autophagy in the fission yeast Schizosaccharomyces pombe. Using this marker, 13 Atg homologues were also found to be required for autophagy in fission yeast. In budding yeast, autophagy-deficient mutants are known to be sterile, whereas in fission yeast we found that up to 30% of autophagy-defective cells with amino acid auxotrophy were able to recover sporulation when an excess of required amino acids was supplied. Furthermore, we found that approximately 15% of the autophagy-defective cells were also able to sporulate when a prototrophic strain was subjected to nitrogen starvation, which suggested that fission yeast may store sufficient intracellular nitrogen to allow partial sporulation under nitrogen-limiting conditions, although the majority of the nitrogen source is supplied by autophagy. Monitoring of the sporulation process revealed that the process was blocked non-specifically at various stages in the atg1 Delta and atg12 Delta mutants, possibly due to a shortage of amino acids. Taking advantage of this partial sporulation ability of fission yeast, we sought evidence for the existence of a recycling system for nitrogen sources during starvation.
- リンク情報
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- DOI
- https://doi.org/10.1099/mic.0.034389-0
- CiNii Articles
- http://ci.nii.ac.jp/naid/80020743443
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19778961
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000272918200004&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1099/mic.0.034389-0
- ISSN : 1350-0872
- eISSN : 1465-2080
- CiNii Articles ID : 80020743443
- PubMed ID : 19778961
- Web of Science ID : WOS:000272918200004