論文

査読有り
2016年4月

Proper notch activity is necessary for the establishment of proximal cells and differentiation of intermediate, distal, and connecting tubule in Xenopus pronephros development

DEVELOPMENTAL DYNAMICS
  • Tomohisa Katada
  • ,
  • Hiroyuki Sakurai

245
4
開始ページ
472
終了ページ
482
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/dvdy.24386
出版者・発行元
WILEY-BLACKWELL

Background: Notch signaling in pronephros development has been shown to regulate establishment of glomus and proximal tubule, but how Notch signal works on competency of pronephric anlagen during the generation of pronephric components remains to be understood. Results: We investigated how components of pronephros (glomus, proximal tubule, intermediate tubule, distal tubule, and connecting tubule) were generated in Xenopus embryos by timed overactivation and suppression of Notch signaling. Notch activation resulted in expansion of the glomus and disruption of the proximal tubule formation. Inhibition of Notch signaling reduced expression of wt1 and XSMP-30. In addition, when Notch signaling was overactivated at stage 20 on, intermediate, distal, and connecting tubule markers, gremlin and clcnkb, were decreased while Notch down-regulation increased gremlin and clcnkb. Similar changes were observed with segmental markers, cldn19, cldn14, and rhcg on activation or inhibition of Notch. Although Notch did not affect the expression of pan-pronephric progenitor marker, pax2, its activation inhibited lumen formation in the pronephros. Conclusions: Notch signal is essential for glomus and proximal tubule development and inhibition of Notch is critical for the differentiation of the intermediate, distal, and connecting tubule. (C) 2016 Wiley Periodicals, Inc.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1002/dvdy.24386
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000373060700004&DestApp=WOS_CPL

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