2011年8月
Biosynthesis and secretion of mouse cysteine-rich with EGF-like domains 2
FEBS LETTERS
- ,
- ,
- ,
- 巻
- 585
- 号
- 15
- 開始ページ
- 2481
- 終了ページ
- 2487
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.febslet.2011.06.029
- 出版者・発行元
- ELSEVIER SCIENCE BV
In this study, we found that Cysteine-rich with EGF-like domains 2 (CRELD2), a novel endoplasmic reticulum stress-inducible protein, is not only localized in the ER-Golgi apparatus but also spontaneously secreted. Deletion of four C-terminal amino acids from mouse CRELD2 or addition of tag-peptides to its C-terminus dramatically enhanced CRELD2 secretion. Intra-and extra-cellular CRELD2 is differentially glycosylated and its spontaneous secretion was significantly prevented by overexpression of a dominant negative mutant Sar1 and treatment with brefeldin A. Overexpression of wild-type GRP78 remarkably enhanced the secretion of wild-type but not mutant CRELD2. Our results demonstrate both that CRELD2 is a novel secretory glycoprotein regulated by Sar1 and GRP78 and that the C-terminal of CRELD2 plays a crucial role in its secretion. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.febslet.2011.06.029
- ISSN : 0014-5793
- Web of Science ID : WOS:000293276000014